A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization

COVID-19 has globally spread to burden the medical system. Even with a massive vaccination, a mucosal vaccine offering more comprehensive and convenient protection is imminent. Here, a micro-sized vaccine based on recombinant Lactiplantibacillus plantarum (rLP) displaying spike or receptor-binding d...

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Autores principales: Li, Letian, Hao, Jiayi, Jiang, Yuhang, Hao, Pengfei, Gao, Yuwei, Chen, Jing, Zhang, Guoqing, Jin, Ningyi, Wang, Maopeng, Li, Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946889/
https://www.ncbi.nlm.nih.gov/pubmed/36852097
http://dx.doi.org/10.1016/j.apsb.2023.01.005
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author Li, Letian
Hao, Jiayi
Jiang, Yuhang
Hao, Pengfei
Gao, Yuwei
Chen, Jing
Zhang, Guoqing
Jin, Ningyi
Wang, Maopeng
Li, Chang
author_facet Li, Letian
Hao, Jiayi
Jiang, Yuhang
Hao, Pengfei
Gao, Yuwei
Chen, Jing
Zhang, Guoqing
Jin, Ningyi
Wang, Maopeng
Li, Chang
author_sort Li, Letian
collection PubMed
description COVID-19 has globally spread to burden the medical system. Even with a massive vaccination, a mucosal vaccine offering more comprehensive and convenient protection is imminent. Here, a micro-sized vaccine based on recombinant Lactiplantibacillus plantarum (rLP) displaying spike or receptor-binding domain (RBD) was characterized as microparticles, and its safety and protective effects against SARS-CoV-2 were evaluated. We found a 66.7% mortality reduction and 100% protection with rLP against SARS-CoV-2 in a mouse model. The histological analysis showed decreased hemorrhage symptoms and increased leukocyte infiltration in the lung. Especially, rLP:RBD significantly decreased pulmonary viral loads. For the first time, our study provides a L. plantarum-vectored vaccine to prevent COVID-19 progress and transmission via intranasal vaccination.
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spelling pubmed-99468892023-02-23 A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization Li, Letian Hao, Jiayi Jiang, Yuhang Hao, Pengfei Gao, Yuwei Chen, Jing Zhang, Guoqing Jin, Ningyi Wang, Maopeng Li, Chang Acta Pharm Sin B Short Communication COVID-19 has globally spread to burden the medical system. Even with a massive vaccination, a mucosal vaccine offering more comprehensive and convenient protection is imminent. Here, a micro-sized vaccine based on recombinant Lactiplantibacillus plantarum (rLP) displaying spike or receptor-binding domain (RBD) was characterized as microparticles, and its safety and protective effects against SARS-CoV-2 were evaluated. We found a 66.7% mortality reduction and 100% protection with rLP against SARS-CoV-2 in a mouse model. The histological analysis showed decreased hemorrhage symptoms and increased leukocyte infiltration in the lung. Especially, rLP:RBD significantly decreased pulmonary viral loads. For the first time, our study provides a L. plantarum-vectored vaccine to prevent COVID-19 progress and transmission via intranasal vaccination. Elsevier 2023-07 2023-02-23 /pmc/articles/PMC9946889/ /pubmed/36852097 http://dx.doi.org/10.1016/j.apsb.2023.01.005 Text en © 2023 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Li, Letian
Hao, Jiayi
Jiang, Yuhang
Hao, Pengfei
Gao, Yuwei
Chen, Jing
Zhang, Guoqing
Jin, Ningyi
Wang, Maopeng
Li, Chang
A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization
title A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization
title_full A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization
title_fullStr A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization
title_full_unstemmed A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization
title_short A micro-sized vaccine based on recombinant Lactiplantibacillus plantarum fights against SARS-CoV-2 infection via intranasal immunization
title_sort micro-sized vaccine based on recombinant lactiplantibacillus plantarum fights against sars-cov-2 infection via intranasal immunization
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946889/
https://www.ncbi.nlm.nih.gov/pubmed/36852097
http://dx.doi.org/10.1016/j.apsb.2023.01.005
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