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PAK2 is essential for chromosome alignment in metaphase I oocytes
As a highly conserved and ubiquitously expressed serine/threonine kinase, p21-activated kinase 2 (PAK2) participates in diverse biologic events. However, its roles in mouse oocyte meiotic maturation remain unclear. The present study revealed that mouse oocytes depleted of Pak2 were unable to complet...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947007/ https://www.ncbi.nlm.nih.gov/pubmed/36813765 http://dx.doi.org/10.1038/s41419-023-05585-7 |
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author | Zeng, Juan Wang, Shiwei Gao, Min Lu, Dian Song, Shuang Chen, Diyu Fan, Weimin Xu, Zhiliang Zhang, Zhiguo Sun, Xiaofang |
author_facet | Zeng, Juan Wang, Shiwei Gao, Min Lu, Dian Song, Shuang Chen, Diyu Fan, Weimin Xu, Zhiliang Zhang, Zhiguo Sun, Xiaofang |
author_sort | Zeng, Juan |
collection | PubMed |
description | As a highly conserved and ubiquitously expressed serine/threonine kinase, p21-activated kinase 2 (PAK2) participates in diverse biologic events. However, its roles in mouse oocyte meiotic maturation remain unclear. The present study revealed that mouse oocytes depleted of Pak2 were unable to completely progress through meiosis and that a majority were arrested at metaphase I. Pak2 depletion thus prompted MI arrest and induced meiotic chromosome alignment defects in mouse oocytes, in part due to a reduction in polo-like kinase (PLK1). We demonstrated that PAK2’s interaction with PLK1 protected it from degradation by APC/C(Cdh1), and that it promoted meiotic progression and bipolar spindle formation. Our data collectively display critical functions for PAK2 in meiotic progression and chromosome alignment in mouse oocytes. |
format | Online Article Text |
id | pubmed-9947007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99470072023-02-24 PAK2 is essential for chromosome alignment in metaphase I oocytes Zeng, Juan Wang, Shiwei Gao, Min Lu, Dian Song, Shuang Chen, Diyu Fan, Weimin Xu, Zhiliang Zhang, Zhiguo Sun, Xiaofang Cell Death Dis Article As a highly conserved and ubiquitously expressed serine/threonine kinase, p21-activated kinase 2 (PAK2) participates in diverse biologic events. However, its roles in mouse oocyte meiotic maturation remain unclear. The present study revealed that mouse oocytes depleted of Pak2 were unable to completely progress through meiosis and that a majority were arrested at metaphase I. Pak2 depletion thus prompted MI arrest and induced meiotic chromosome alignment defects in mouse oocytes, in part due to a reduction in polo-like kinase (PLK1). We demonstrated that PAK2’s interaction with PLK1 protected it from degradation by APC/C(Cdh1), and that it promoted meiotic progression and bipolar spindle formation. Our data collectively display critical functions for PAK2 in meiotic progression and chromosome alignment in mouse oocytes. Nature Publishing Group UK 2023-02-22 /pmc/articles/PMC9947007/ /pubmed/36813765 http://dx.doi.org/10.1038/s41419-023-05585-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zeng, Juan Wang, Shiwei Gao, Min Lu, Dian Song, Shuang Chen, Diyu Fan, Weimin Xu, Zhiliang Zhang, Zhiguo Sun, Xiaofang PAK2 is essential for chromosome alignment in metaphase I oocytes |
title | PAK2 is essential for chromosome alignment in metaphase I oocytes |
title_full | PAK2 is essential for chromosome alignment in metaphase I oocytes |
title_fullStr | PAK2 is essential for chromosome alignment in metaphase I oocytes |
title_full_unstemmed | PAK2 is essential for chromosome alignment in metaphase I oocytes |
title_short | PAK2 is essential for chromosome alignment in metaphase I oocytes |
title_sort | pak2 is essential for chromosome alignment in metaphase i oocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947007/ https://www.ncbi.nlm.nih.gov/pubmed/36813765 http://dx.doi.org/10.1038/s41419-023-05585-7 |
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