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CRISPR-based large-scale modeling of loss-of-function mutations to investigate mechanisms of stress resistance in cancer
Dissecting mechanisms driving subclone expansion in primary cancers has been challenging. Here, we present a protocol to systematically disrupt entire gene networks and assess the functional impact of this perturbation on cancer cell fitness. By combining arrayed CRISPR libraries and high-content mi...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947008/ https://www.ncbi.nlm.nih.gov/pubmed/36853711 http://dx.doi.org/10.1016/j.xpro.2023.102097 |