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Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts

Cold plasma treatment has been studied to enhance the germination, growth, and bioactive phytochemical production in crops. Here, we aimed to investigate the effects of cold plasma treatment on the growth, bioactive metabolite production, and protein expression related to the physiological and osteo...

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Autores principales: Lee, Mi Ja, Lee, Hyun-Jin, Lee, Yongjin, Yang, Ji Yeong, Song, Jong Seok, Woo, So Yeun, Kim, Hyun Young, Song, Seung-Yeob, Seo, Woo Duck, Son, Young-Jin, Park, Sung Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947073/
https://www.ncbi.nlm.nih.gov/pubmed/36380140
http://dx.doi.org/10.1007/s11130-022-01029-3
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author Lee, Mi Ja
Lee, Hyun-Jin
Lee, Yongjin
Yang, Ji Yeong
Song, Jong Seok
Woo, So Yeun
Kim, Hyun Young
Song, Seung-Yeob
Seo, Woo Duck
Son, Young-Jin
Park, Sung Il
author_facet Lee, Mi Ja
Lee, Hyun-Jin
Lee, Yongjin
Yang, Ji Yeong
Song, Jong Seok
Woo, So Yeun
Kim, Hyun Young
Song, Seung-Yeob
Seo, Woo Duck
Son, Young-Jin
Park, Sung Il
author_sort Lee, Mi Ja
collection PubMed
description Cold plasma treatment has been studied to enhance the germination, growth, and bioactive phytochemical production in crops. Here, we aimed to investigate the effects of cold plasma treatment on the growth, bioactive metabolite production, and protein expression related to the physiological and osteogenic activities of oat sprouts. Oat seeds were soaked for 12 h, and then exposed to plasma for 6 min/day for 3 days after sowing. Plasma exposure did not significantly change the growth of oat sprouts; however, increased the content of bioactive metabolites. A single exposure for 6 min on the first day (T-1) increased the content of free amino acids (39.4%), γ-aminobutyric acid (53%), and avenacoside B (23%) compared to the control. Hexacosanol content was the highest in T-3 (6 min exposure on each day for 3 days), 28% higher than that in the control. Oat sprout extracts induced the phosphorylation of adenosine 5′-monophosphate-activated protein kinase and osteoblast differentiation was enhanced by increasing the alkaline phosphatase (ALP) activity; all these effects were induced by plasma treatment. Avenacoside B content was positively correlated with ALP activity (r = 0.911, p < 0.1). These results suggest that plasma treatment has the potential to improve the value of oat sprouts and that it may be used in food fortification to enhance nutritional value for promoting human health. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11130-022-01029-3.
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spelling pubmed-99470732023-02-24 Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts Lee, Mi Ja Lee, Hyun-Jin Lee, Yongjin Yang, Ji Yeong Song, Jong Seok Woo, So Yeun Kim, Hyun Young Song, Seung-Yeob Seo, Woo Duck Son, Young-Jin Park, Sung Il Plant Foods Hum Nutr Research Cold plasma treatment has been studied to enhance the germination, growth, and bioactive phytochemical production in crops. Here, we aimed to investigate the effects of cold plasma treatment on the growth, bioactive metabolite production, and protein expression related to the physiological and osteogenic activities of oat sprouts. Oat seeds were soaked for 12 h, and then exposed to plasma for 6 min/day for 3 days after sowing. Plasma exposure did not significantly change the growth of oat sprouts; however, increased the content of bioactive metabolites. A single exposure for 6 min on the first day (T-1) increased the content of free amino acids (39.4%), γ-aminobutyric acid (53%), and avenacoside B (23%) compared to the control. Hexacosanol content was the highest in T-3 (6 min exposure on each day for 3 days), 28% higher than that in the control. Oat sprout extracts induced the phosphorylation of adenosine 5′-monophosphate-activated protein kinase and osteoblast differentiation was enhanced by increasing the alkaline phosphatase (ALP) activity; all these effects were induced by plasma treatment. Avenacoside B content was positively correlated with ALP activity (r = 0.911, p < 0.1). These results suggest that plasma treatment has the potential to improve the value of oat sprouts and that it may be used in food fortification to enhance nutritional value for promoting human health. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11130-022-01029-3. Springer US 2022-11-16 2023 /pmc/articles/PMC9947073/ /pubmed/36380140 http://dx.doi.org/10.1007/s11130-022-01029-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Lee, Mi Ja
Lee, Hyun-Jin
Lee, Yongjin
Yang, Ji Yeong
Song, Jong Seok
Woo, So Yeun
Kim, Hyun Young
Song, Seung-Yeob
Seo, Woo Duck
Son, Young-Jin
Park, Sung Il
Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts
title Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts
title_full Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts
title_fullStr Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts
title_full_unstemmed Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts
title_short Cold Plasma Treatment Increases Bioactive Metabolites in Oat (Avena sativa L.) Sprouts and Enhances In Vitro Osteogenic Activity of their Extracts
title_sort cold plasma treatment increases bioactive metabolites in oat (avena sativa l.) sprouts and enhances in vitro osteogenic activity of their extracts
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947073/
https://www.ncbi.nlm.nih.gov/pubmed/36380140
http://dx.doi.org/10.1007/s11130-022-01029-3
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