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Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization

Direct mass spectrometry (MS) analysis of human tissue at the molecular level could gain insight into biomarker discovery and disease diagnosis. Detecting metabolite profiles of tissue sample play an important role in understanding the pathological properties of disease development. Because the comp...

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Autores principales: Liu, Dasheng, Shen, Yuejian, Di, Dandan, Cai, Shenhui, Huang, Xueyang, Lin, Hongguo, Huang, Yalan, Xue, Jing, Liu, Li, Hu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947238/
https://www.ncbi.nlm.nih.gov/pubmed/36846859
http://dx.doi.org/10.3389/fchem.2023.1134948
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author Liu, Dasheng
Shen, Yuejian
Di, Dandan
Cai, Shenhui
Huang, Xueyang
Lin, Hongguo
Huang, Yalan
Xue, Jing
Liu, Li
Hu, Bin
author_facet Liu, Dasheng
Shen, Yuejian
Di, Dandan
Cai, Shenhui
Huang, Xueyang
Lin, Hongguo
Huang, Yalan
Xue, Jing
Liu, Li
Hu, Bin
author_sort Liu, Dasheng
collection PubMed
description Direct mass spectrometry (MS) analysis of human tissue at the molecular level could gain insight into biomarker discovery and disease diagnosis. Detecting metabolite profiles of tissue sample play an important role in understanding the pathological properties of disease development. Because the complex matrices in tissue samples, complicated and time-consuming sample preparation processes are usually required by conventional biological and clinical MS methods. Direct MS with ambient ionization technique is a new analytical strategy for direct sample analysis with little sample preparation, and has been proven to be a simple, rapid, and effective analytical tools for direct analysis of biological tissues. In this work, we applied a simple, low-cost, disposable wooden tip (WT) for loading tiny thyroid tissue, and then loading organic solvents to extract biomarkers under electrospray ionization (ESI) condition. Under such WT-ESI, the extract of thyroid was directly sprayed out from wooden tip to MS inlet. In this work, thyroid tissue from normal and cancer parts were analyzed by the established WT-ESI-MS, showing lipids were mainly detectable compounds in thyroid tissue. The MS data of lipids obtained from thyroid tissues were further analyzed with MS/MS experiment and multivariate variable analysis, and the biomarkers of thyroid cancer were also investigated.
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spelling pubmed-99472382023-02-24 Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization Liu, Dasheng Shen, Yuejian Di, Dandan Cai, Shenhui Huang, Xueyang Lin, Hongguo Huang, Yalan Xue, Jing Liu, Li Hu, Bin Front Chem Chemistry Direct mass spectrometry (MS) analysis of human tissue at the molecular level could gain insight into biomarker discovery and disease diagnosis. Detecting metabolite profiles of tissue sample play an important role in understanding the pathological properties of disease development. Because the complex matrices in tissue samples, complicated and time-consuming sample preparation processes are usually required by conventional biological and clinical MS methods. Direct MS with ambient ionization technique is a new analytical strategy for direct sample analysis with little sample preparation, and has been proven to be a simple, rapid, and effective analytical tools for direct analysis of biological tissues. In this work, we applied a simple, low-cost, disposable wooden tip (WT) for loading tiny thyroid tissue, and then loading organic solvents to extract biomarkers under electrospray ionization (ESI) condition. Under such WT-ESI, the extract of thyroid was directly sprayed out from wooden tip to MS inlet. In this work, thyroid tissue from normal and cancer parts were analyzed by the established WT-ESI-MS, showing lipids were mainly detectable compounds in thyroid tissue. The MS data of lipids obtained from thyroid tissues were further analyzed with MS/MS experiment and multivariate variable analysis, and the biomarkers of thyroid cancer were also investigated. Frontiers Media S.A. 2023-02-09 /pmc/articles/PMC9947238/ /pubmed/36846859 http://dx.doi.org/10.3389/fchem.2023.1134948 Text en Copyright © 2023 Liu, Shen, Di, Cai, Huang, Lin, Huang, Xue, Liu and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Liu, Dasheng
Shen, Yuejian
Di, Dandan
Cai, Shenhui
Huang, Xueyang
Lin, Hongguo
Huang, Yalan
Xue, Jing
Liu, Li
Hu, Bin
Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization
title Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization
title_full Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization
title_fullStr Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization
title_full_unstemmed Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization
title_short Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization
title_sort direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947238/
https://www.ncbi.nlm.nih.gov/pubmed/36846859
http://dx.doi.org/10.3389/fchem.2023.1134948
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