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Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater

Industrialization leads to the entry of diverse xenobiotic compounds into the environment. One such compound is paracetamol (APAP), which is emerging as a pharmaceutical and personal care pollutant (PPCP). In this study, the APAP degrading bacterium was isolated by enrichment culture method from the...

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Autores principales: Chopra, Sunil, Kumar, Dharmender
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947448/
https://www.ncbi.nlm.nih.gov/pubmed/36852133
http://dx.doi.org/10.1007/s12210-023-01140-w
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author Chopra, Sunil
Kumar, Dharmender
author_facet Chopra, Sunil
Kumar, Dharmender
author_sort Chopra, Sunil
collection PubMed
description Industrialization leads to the entry of diverse xenobiotic compounds into the environment. One such compound is paracetamol (APAP), which is emerging as a pharmaceutical and personal care pollutant (PPCP). In this study, the APAP degrading bacterium was isolated by enrichment culture method from the sewage sample. The microscopy, biochemical, and 16S rRNA gene sequence analyzed the isolate PPY-2, which belongs to Bacillus licheniformis, and GenBank assigned accession number MN744328. Physiological and batch culture degradation studies have indicated that the strain involved in the degradation of APAP. The optimum pH for degradation of the PPY-2 was 7.7, whereas the temperature was 25 °C, agitation speed was 142 rpm, and concentration of APAP was 621 mg/L reported, and the optimum temperatures were 42 °C and 32 °C, respectively. Biomass kinetic was studied at optimal physical conditions, which suggested that the specific growth rate (μ) was 721 mg/L. The GC–MS chromatogram peaks have detected metabolites, viz., oxalic acid, 2-isopropyl-5-methyl cyclohexanone, and phenothiazine. The study confirmed that Bacillus licheniformis strain PPY-2 exhibits metabolic potential to biodegradation APAP and can be further deployed in bioremediation.
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spelling pubmed-99474482023-02-23 Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater Chopra, Sunil Kumar, Dharmender Rend Lincei Sci Fis Nat Research Paper Industrialization leads to the entry of diverse xenobiotic compounds into the environment. One such compound is paracetamol (APAP), which is emerging as a pharmaceutical and personal care pollutant (PPCP). In this study, the APAP degrading bacterium was isolated by enrichment culture method from the sewage sample. The microscopy, biochemical, and 16S rRNA gene sequence analyzed the isolate PPY-2, which belongs to Bacillus licheniformis, and GenBank assigned accession number MN744328. Physiological and batch culture degradation studies have indicated that the strain involved in the degradation of APAP. The optimum pH for degradation of the PPY-2 was 7.7, whereas the temperature was 25 °C, agitation speed was 142 rpm, and concentration of APAP was 621 mg/L reported, and the optimum temperatures were 42 °C and 32 °C, respectively. Biomass kinetic was studied at optimal physical conditions, which suggested that the specific growth rate (μ) was 721 mg/L. The GC–MS chromatogram peaks have detected metabolites, viz., oxalic acid, 2-isopropyl-5-methyl cyclohexanone, and phenothiazine. The study confirmed that Bacillus licheniformis strain PPY-2 exhibits metabolic potential to biodegradation APAP and can be further deployed in bioremediation. Springer International Publishing 2023-02-23 2023 /pmc/articles/PMC9947448/ /pubmed/36852133 http://dx.doi.org/10.1007/s12210-023-01140-w Text en © The Author(s), under exclusive licence to Accademia Nazionale dei Lincei 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Paper
Chopra, Sunil
Kumar, Dharmender
Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater
title Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater
title_full Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater
title_fullStr Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater
title_full_unstemmed Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater
title_short Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater
title_sort characterization and biodegradation of paracetamol by biomass of bacillus licheniformis strain ppy-2 isolated from wastewater
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947448/
https://www.ncbi.nlm.nih.gov/pubmed/36852133
http://dx.doi.org/10.1007/s12210-023-01140-w
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