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Re-evaluating the impact of alternative RNA splicing on proteomic diversity
Alternative splicing (AS) constitutes a mechanism by which protein-coding genes and long non-coding RNA (lncRNA) genes produce more than a single mature transcript. From plants to humans, AS is a powerful process that increases transcriptome complexity. Importantly, splice variants produced from AS...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947481/ https://www.ncbi.nlm.nih.gov/pubmed/36845399 http://dx.doi.org/10.3389/fgene.2023.1089053 |
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author | Manuel, Jeru Manoj Guilloy, Noé Khatir, Inès Roucou, Xavier Laurent, Benoit |
author_facet | Manuel, Jeru Manoj Guilloy, Noé Khatir, Inès Roucou, Xavier Laurent, Benoit |
author_sort | Manuel, Jeru Manoj |
collection | PubMed |
description | Alternative splicing (AS) constitutes a mechanism by which protein-coding genes and long non-coding RNA (lncRNA) genes produce more than a single mature transcript. From plants to humans, AS is a powerful process that increases transcriptome complexity. Importantly, splice variants produced from AS can potentially encode for distinct protein isoforms which can lose or gain specific domains and, hence, differ in their functional properties. Advances in proteomics have shown that the proteome is indeed diverse due to the presence of numerous protein isoforms. For the past decades, with the help of advanced high-throughput technologies, numerous alternatively spliced transcripts have been identified. However, the low detection rate of protein isoforms in proteomic studies raised debatable questions on whether AS contributes to proteomic diversity and on how many AS events are really functional. We propose here to assess and discuss the impact of AS on proteomic complexity in the light of the technological progress, updated genome annotation, and current scientific knowledge. |
format | Online Article Text |
id | pubmed-9947481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99474812023-02-24 Re-evaluating the impact of alternative RNA splicing on proteomic diversity Manuel, Jeru Manoj Guilloy, Noé Khatir, Inès Roucou, Xavier Laurent, Benoit Front Genet Genetics Alternative splicing (AS) constitutes a mechanism by which protein-coding genes and long non-coding RNA (lncRNA) genes produce more than a single mature transcript. From plants to humans, AS is a powerful process that increases transcriptome complexity. Importantly, splice variants produced from AS can potentially encode for distinct protein isoforms which can lose or gain specific domains and, hence, differ in their functional properties. Advances in proteomics have shown that the proteome is indeed diverse due to the presence of numerous protein isoforms. For the past decades, with the help of advanced high-throughput technologies, numerous alternatively spliced transcripts have been identified. However, the low detection rate of protein isoforms in proteomic studies raised debatable questions on whether AS contributes to proteomic diversity and on how many AS events are really functional. We propose here to assess and discuss the impact of AS on proteomic complexity in the light of the technological progress, updated genome annotation, and current scientific knowledge. Frontiers Media S.A. 2023-02-09 /pmc/articles/PMC9947481/ /pubmed/36845399 http://dx.doi.org/10.3389/fgene.2023.1089053 Text en Copyright © 2023 Manuel, Guilloy, Khatir, Roucou and Laurent. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Manuel, Jeru Manoj Guilloy, Noé Khatir, Inès Roucou, Xavier Laurent, Benoit Re-evaluating the impact of alternative RNA splicing on proteomic diversity |
title | Re-evaluating the impact of alternative RNA splicing on proteomic diversity |
title_full | Re-evaluating the impact of alternative RNA splicing on proteomic diversity |
title_fullStr | Re-evaluating the impact of alternative RNA splicing on proteomic diversity |
title_full_unstemmed | Re-evaluating the impact of alternative RNA splicing on proteomic diversity |
title_short | Re-evaluating the impact of alternative RNA splicing on proteomic diversity |
title_sort | re-evaluating the impact of alternative rna splicing on proteomic diversity |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947481/ https://www.ncbi.nlm.nih.gov/pubmed/36845399 http://dx.doi.org/10.3389/fgene.2023.1089053 |
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