Detection of Zebrafish Retinal Proteins by Infrared Western Blotting

The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research. This protocol describes a method to quantitatively evaluate the expression of Arrestin3a (Arr3a) a...

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Detalles Bibliográficos
Autores principales: Zang, Jingjing, Neuhauss, Stephan C. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947546/
https://www.ncbi.nlm.nih.gov/pubmed/36845534
http://dx.doi.org/10.21769/BioProtoc.4618
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author Zang, Jingjing
Neuhauss, Stephan C. F.
author_facet Zang, Jingjing
Neuhauss, Stephan C. F.
author_sort Zang, Jingjing
collection PubMed
description The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research. This protocol describes a method to quantitatively evaluate the expression of Arrestin3a (Arr3a) and G-protein receptor kinase7a (Grk7a) in the adult zebrafish retina at protein levels by infrared fluorescence western blot. Our protocol can be easily adapted to measure protein levels in additional zebrafish tissues.
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spelling pubmed-99475462023-02-24 Detection of Zebrafish Retinal Proteins by Infrared Western Blotting Zang, Jingjing Neuhauss, Stephan C. F. Bio Protoc Methods Article The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research. This protocol describes a method to quantitatively evaluate the expression of Arrestin3a (Arr3a) and G-protein receptor kinase7a (Grk7a) in the adult zebrafish retina at protein levels by infrared fluorescence western blot. Our protocol can be easily adapted to measure protein levels in additional zebrafish tissues. Bio-Protocol 2023-02-20 /pmc/articles/PMC9947546/ /pubmed/36845534 http://dx.doi.org/10.21769/BioProtoc.4618 Text en Copyright © 2023 The Authors; exclusive licensee Bio-protocol LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Methods Article
Zang, Jingjing
Neuhauss, Stephan C. F.
Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
title Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
title_full Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
title_fullStr Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
title_full_unstemmed Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
title_short Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
title_sort detection of zebrafish retinal proteins by infrared western blotting
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947546/
https://www.ncbi.nlm.nih.gov/pubmed/36845534
http://dx.doi.org/10.21769/BioProtoc.4618
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