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Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum

Lake Erie is subject to recurring events of cyanobacterial harmful algal blooms (cHABs), but measures of nutrients and total phytoplankton biomass seem to be poor predictors of cHABs when taken individually. A more integrated approach at the watershed scale may improve our understanding of the condi...

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Autores principales: Crevecoeur, Sophie, Edge, Thomas A., Watson, Linet Cynthia, Watson, Susan B., Greer, Charles W., Ciborowski, Jan J. H., Diep, Ngan, Dove, Alice, Drouillard, Kenneth G., Frenken, Thijs, McKay, Robert Michael, Zastepa, Arthur, Comte, Jérôme
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947797/
https://www.ncbi.nlm.nih.gov/pubmed/36846788
http://dx.doi.org/10.3389/fmicb.2023.1073753
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author Crevecoeur, Sophie
Edge, Thomas A.
Watson, Linet Cynthia
Watson, Susan B.
Greer, Charles W.
Ciborowski, Jan J. H.
Diep, Ngan
Dove, Alice
Drouillard, Kenneth G.
Frenken, Thijs
McKay, Robert Michael
Zastepa, Arthur
Comte, Jérôme
author_facet Crevecoeur, Sophie
Edge, Thomas A.
Watson, Linet Cynthia
Watson, Susan B.
Greer, Charles W.
Ciborowski, Jan J. H.
Diep, Ngan
Dove, Alice
Drouillard, Kenneth G.
Frenken, Thijs
McKay, Robert Michael
Zastepa, Arthur
Comte, Jérôme
author_sort Crevecoeur, Sophie
collection PubMed
description Lake Erie is subject to recurring events of cyanobacterial harmful algal blooms (cHABs), but measures of nutrients and total phytoplankton biomass seem to be poor predictors of cHABs when taken individually. A more integrated approach at the watershed scale may improve our understanding of the conditions that lead to bloom formation, such as assessing the physico-chemical and biological factors that influence the lake microbial community, as well as identifying the linkages between Lake Erie and the surrounding watershed. Within the scope of the Government of Canada’s Genomics Research and Development Initiative (GRDI) Ecobiomics project, we used high-throughput sequencing of the 16S rRNA gene to characterize the spatio-temporal variability of the aquatic microbiome in the Thames River–Lake St. Clair-Detroit River–Lake Erie aquatic corridor. We found that the aquatic microbiome was structured along the flow path and influenced mainly by higher nutrient concentrations in the Thames River, and higher temperature and pH downstream in Lake St. Clair and Lake Erie. The same dominant bacterial phyla were detected along the water continuum, changing only in relative abundance. At finer taxonomical level, however, there was a clear shift in the cyanobacterial community, with Planktothrix dominating in the Thames River and Microcystis and Synechococcus in Lake St. Clair and Lake Erie. Mantel correlations highlighted the importance of geographic distance in shaping the microbial community structure. The fact that a high proportion of microbial sequences found in the Western Basin of Lake Erie were also identified in the Thames River, indicated a high degree of connectivity and dispersal within the system, where mass effect induced by passive transport play an important role in microbial community assembly. Nevertheless, some cyanobacterial amplicon sequence variants (ASVs) related to Microcystis, representing less than 0.1% of relative abundance in the upstream Thames River, became dominant in Lake St. Clair and Erie, suggesting selection of those ASVs based on the lake conditions. Their extremely low relative abundances in the Thames suggest additional sources are likely to contribute to the rapid development of summer and fall blooms in the Western Basin of Lake Erie. Collectively, these results, which can be applied to other watersheds, improve our understanding of the factors influencing aquatic microbial community assembly and provide new perspectives on how to better understand the occurrence of cHABs in Lake Erie and elsewhere.
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spelling pubmed-99477972023-02-24 Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum Crevecoeur, Sophie Edge, Thomas A. Watson, Linet Cynthia Watson, Susan B. Greer, Charles W. Ciborowski, Jan J. H. Diep, Ngan Dove, Alice Drouillard, Kenneth G. Frenken, Thijs McKay, Robert Michael Zastepa, Arthur Comte, Jérôme Front Microbiol Microbiology Lake Erie is subject to recurring events of cyanobacterial harmful algal blooms (cHABs), but measures of nutrients and total phytoplankton biomass seem to be poor predictors of cHABs when taken individually. A more integrated approach at the watershed scale may improve our understanding of the conditions that lead to bloom formation, such as assessing the physico-chemical and biological factors that influence the lake microbial community, as well as identifying the linkages between Lake Erie and the surrounding watershed. Within the scope of the Government of Canada’s Genomics Research and Development Initiative (GRDI) Ecobiomics project, we used high-throughput sequencing of the 16S rRNA gene to characterize the spatio-temporal variability of the aquatic microbiome in the Thames River–Lake St. Clair-Detroit River–Lake Erie aquatic corridor. We found that the aquatic microbiome was structured along the flow path and influenced mainly by higher nutrient concentrations in the Thames River, and higher temperature and pH downstream in Lake St. Clair and Lake Erie. The same dominant bacterial phyla were detected along the water continuum, changing only in relative abundance. At finer taxonomical level, however, there was a clear shift in the cyanobacterial community, with Planktothrix dominating in the Thames River and Microcystis and Synechococcus in Lake St. Clair and Lake Erie. Mantel correlations highlighted the importance of geographic distance in shaping the microbial community structure. The fact that a high proportion of microbial sequences found in the Western Basin of Lake Erie were also identified in the Thames River, indicated a high degree of connectivity and dispersal within the system, where mass effect induced by passive transport play an important role in microbial community assembly. Nevertheless, some cyanobacterial amplicon sequence variants (ASVs) related to Microcystis, representing less than 0.1% of relative abundance in the upstream Thames River, became dominant in Lake St. Clair and Erie, suggesting selection of those ASVs based on the lake conditions. Their extremely low relative abundances in the Thames suggest additional sources are likely to contribute to the rapid development of summer and fall blooms in the Western Basin of Lake Erie. Collectively, these results, which can be applied to other watersheds, improve our understanding of the factors influencing aquatic microbial community assembly and provide new perspectives on how to better understand the occurrence of cHABs in Lake Erie and elsewhere. Frontiers Media S.A. 2023-02-09 /pmc/articles/PMC9947797/ /pubmed/36846788 http://dx.doi.org/10.3389/fmicb.2023.1073753 Text en Copyright © 2023 Crevecoeur, Edge, Watson, Watson, Greer, Ciborowski, Diep, Dove, Drouillard, Frenken, McKay, Zastepa and Comte. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Crevecoeur, Sophie
Edge, Thomas A.
Watson, Linet Cynthia
Watson, Susan B.
Greer, Charles W.
Ciborowski, Jan J. H.
Diep, Ngan
Dove, Alice
Drouillard, Kenneth G.
Frenken, Thijs
McKay, Robert Michael
Zastepa, Arthur
Comte, Jérôme
Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum
title Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum
title_full Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum
title_fullStr Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum
title_full_unstemmed Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum
title_short Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum
title_sort spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a great lake riverine-lacustrine continuum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947797/
https://www.ncbi.nlm.nih.gov/pubmed/36846788
http://dx.doi.org/10.3389/fmicb.2023.1073753
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