Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases

[Image: see text] Pseudo-ternary mixtures of lamellar phase phospholipids (DPPC and brain sphingomyelin with cholesterol) were studied below T(m) while comparing the influence of cholesterol content, temperature, and the presence of small quantities of vitamin D binding protein (DBP) or vitamin D re...

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Autores principales: Gater, Deborah L., Hughes, Keontré I., Stojanoff, Vivian, Isakovic, Abdel F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947962/
https://www.ncbi.nlm.nih.gov/pubmed/36844553
http://dx.doi.org/10.1021/acsomega.2c04914
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author Gater, Deborah L.
Hughes, Keontré I.
Stojanoff, Vivian
Isakovic, Abdel F.
author_facet Gater, Deborah L.
Hughes, Keontré I.
Stojanoff, Vivian
Isakovic, Abdel F.
author_sort Gater, Deborah L.
collection PubMed
description [Image: see text] Pseudo-ternary mixtures of lamellar phase phospholipids (DPPC and brain sphingomyelin with cholesterol) were studied below T(m) while comparing the influence of cholesterol content, temperature, and the presence of small quantities of vitamin D binding protein (DBP) or vitamin D receptor (VDR). The measurements, conducted by X-ray diffraction (XRD) and nuclear magnetic resonance (NMR), cover a range of cholesterol concentrations (20% mol. wt to 40% mol. wt.) and physiologically relevant temperature range (294–314 K). In addition to rich intraphase behavior, data and modeling are used to approximate the lipids’ headgroup location variations under the abovementioned experimental conditions.
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spelling pubmed-99479622023-02-24 Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases Gater, Deborah L. Hughes, Keontré I. Stojanoff, Vivian Isakovic, Abdel F. ACS Omega [Image: see text] Pseudo-ternary mixtures of lamellar phase phospholipids (DPPC and brain sphingomyelin with cholesterol) were studied below T(m) while comparing the influence of cholesterol content, temperature, and the presence of small quantities of vitamin D binding protein (DBP) or vitamin D receptor (VDR). The measurements, conducted by X-ray diffraction (XRD) and nuclear magnetic resonance (NMR), cover a range of cholesterol concentrations (20% mol. wt to 40% mol. wt.) and physiologically relevant temperature range (294–314 K). In addition to rich intraphase behavior, data and modeling are used to approximate the lipids’ headgroup location variations under the abovementioned experimental conditions. American Chemical Society 2023-02-08 /pmc/articles/PMC9947962/ /pubmed/36844553 http://dx.doi.org/10.1021/acsomega.2c04914 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Gater, Deborah L.
Hughes, Keontré I.
Stojanoff, Vivian
Isakovic, Abdel F.
Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases
title Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases
title_full Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases
title_fullStr Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases
title_full_unstemmed Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases
title_short Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases
title_sort phase heterogeneity in cholesterol-containing ternary phospholipid lamellar phases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9947962/
https://www.ncbi.nlm.nih.gov/pubmed/36844553
http://dx.doi.org/10.1021/acsomega.2c04914
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