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Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways

Stem growth and development has considerable effects on plant architecture and yield performance. Strigolactones (SLs) modulate shoot branching and root architecture in plants. However, the molecular mechanisms underlying SLs regulate cherry rootstocks stem growth and development remain unclear. Our...

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Autores principales: Liu, Xunju, Xu, Yan, Sun, Wanxia, Wang, Jiyuan, Gao, Yixin, Wang, Lei, Xu, Wenping, Wang, Shiping, Jiu, Songtao, Zhang, Caixi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9948674/
https://www.ncbi.nlm.nih.gov/pubmed/36844087
http://dx.doi.org/10.3389/fpls.2023.1092654
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author Liu, Xunju
Xu, Yan
Sun, Wanxia
Wang, Jiyuan
Gao, Yixin
Wang, Lei
Xu, Wenping
Wang, Shiping
Jiu, Songtao
Zhang, Caixi
author_facet Liu, Xunju
Xu, Yan
Sun, Wanxia
Wang, Jiyuan
Gao, Yixin
Wang, Lei
Xu, Wenping
Wang, Shiping
Jiu, Songtao
Zhang, Caixi
author_sort Liu, Xunju
collection PubMed
description Stem growth and development has considerable effects on plant architecture and yield performance. Strigolactones (SLs) modulate shoot branching and root architecture in plants. However, the molecular mechanisms underlying SLs regulate cherry rootstocks stem growth and development remain unclear. Our studies showed that the synthetic SL analog rac-GR24 and the biosynthetic inhibitor TIS108 affected stem length and diameter, aboveground weight, and chlorophyll content. The stem length of cherry rootstocks following TIS108 treatment reached a maximum value of 6.97 cm, which was much higher than that following rac-GR24 treatments at 30 days after treatment. Stem paraffin section showed that SLs affected cell size. A total of 1936, 743, and 1656 differentially expressed genes (DEGs) were observed in stems treated with 10 μM rac-GR24, 0.1 μM rac-GR24, and 10 μM TIS108, respectively. RNA-seq results highlighted several DEGs, including CKX, LOG, YUCCA, AUX, and EXP, which play vital roles in stem growth and development. UPLC-3Q-MS analysis revealed that SL analogs and inhibitors affected the levels of several hormones in the stems. The endogenous GA(3) content of stems increased significantly with 0.1 μM rac-GR24 or 10 μM TIS108 treatment, which is consistent with changes in the stem length following the same treatments. This study demonstrated that SLs affected stem growth of cherry rootstocks by changing other endogenous hormone levels. These results provide a solid theoretical basis for using SLs to modulate plant height and achieve sweet cherry dwarfing and high-density cultivation.
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spelling pubmed-99486742023-02-24 Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways Liu, Xunju Xu, Yan Sun, Wanxia Wang, Jiyuan Gao, Yixin Wang, Lei Xu, Wenping Wang, Shiping Jiu, Songtao Zhang, Caixi Front Plant Sci Plant Science Stem growth and development has considerable effects on plant architecture and yield performance. Strigolactones (SLs) modulate shoot branching and root architecture in plants. However, the molecular mechanisms underlying SLs regulate cherry rootstocks stem growth and development remain unclear. Our studies showed that the synthetic SL analog rac-GR24 and the biosynthetic inhibitor TIS108 affected stem length and diameter, aboveground weight, and chlorophyll content. The stem length of cherry rootstocks following TIS108 treatment reached a maximum value of 6.97 cm, which was much higher than that following rac-GR24 treatments at 30 days after treatment. Stem paraffin section showed that SLs affected cell size. A total of 1936, 743, and 1656 differentially expressed genes (DEGs) were observed in stems treated with 10 μM rac-GR24, 0.1 μM rac-GR24, and 10 μM TIS108, respectively. RNA-seq results highlighted several DEGs, including CKX, LOG, YUCCA, AUX, and EXP, which play vital roles in stem growth and development. UPLC-3Q-MS analysis revealed that SL analogs and inhibitors affected the levels of several hormones in the stems. The endogenous GA(3) content of stems increased significantly with 0.1 μM rac-GR24 or 10 μM TIS108 treatment, which is consistent with changes in the stem length following the same treatments. This study demonstrated that SLs affected stem growth of cherry rootstocks by changing other endogenous hormone levels. These results provide a solid theoretical basis for using SLs to modulate plant height and achieve sweet cherry dwarfing and high-density cultivation. Frontiers Media S.A. 2023-02-09 /pmc/articles/PMC9948674/ /pubmed/36844087 http://dx.doi.org/10.3389/fpls.2023.1092654 Text en Copyright © 2023 Liu, Xu, Sun, Wang, Gao, Wang, Xu, Wang, Jiu and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Xunju
Xu, Yan
Sun, Wanxia
Wang, Jiyuan
Gao, Yixin
Wang, Lei
Xu, Wenping
Wang, Shiping
Jiu, Songtao
Zhang, Caixi
Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
title Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
title_full Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
title_fullStr Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
title_full_unstemmed Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
title_short Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
title_sort strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9948674/
https://www.ncbi.nlm.nih.gov/pubmed/36844087
http://dx.doi.org/10.3389/fpls.2023.1092654
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