Revealing Causes for False-Positive and False-Negative Calling of Gene Essentiality in Escherichia coli Using Transposon Insertion Sequencing

The massive sequencing of transposon insertion mutant libraries (Tn-Seq) represents a commonly used method to determine essential genes in bacteria. Using a hypersaturated transposon mutant library consisting of 400,096 unique Tn insertions, 523 genes were classified as essential in Escherichia coli...

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Detalles Bibliográficos
Autores principales: Choe, Donghui, Kim, Uigi, Hwang, Soonkyu, Seo, Sang Woo, Kim, Donghyuk, Cho, Suhyung, Palsson, Bernhard, Cho, Byung-Kwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Observation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9948719/
https://www.ncbi.nlm.nih.gov/pubmed/36507678
http://dx.doi.org/10.1128/msystems.00896-22

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