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QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus

Mature biofilm formation by Vibrio parahaemolyticus requires exopolysaccharide (EPS), type IV pili, and capsular polysaccharide (CPS). Production of each is strictly regulated by various control pathways including quorum sensing (QS) and bis-(3′–5′)-cyclic di-GMP (c-di-GMP). QsvR, an AraC-type regul...

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Autores principales: Zhang, Miaomiao, Xue, Xingfan, Li, Xue, Wu, Qimin, Zhang, Tingting, Yang, Wenhui, Hu, Lingfei, Zhou, Dongsheng, Lu, Renfei, Zhang, Yiquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9948739/
https://www.ncbi.nlm.nih.gov/pubmed/36846774
http://dx.doi.org/10.3389/fmicb.2023.1079653
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author Zhang, Miaomiao
Xue, Xingfan
Li, Xue
Wu, Qimin
Zhang, Tingting
Yang, Wenhui
Hu, Lingfei
Zhou, Dongsheng
Lu, Renfei
Zhang, Yiquan
author_facet Zhang, Miaomiao
Xue, Xingfan
Li, Xue
Wu, Qimin
Zhang, Tingting
Yang, Wenhui
Hu, Lingfei
Zhou, Dongsheng
Lu, Renfei
Zhang, Yiquan
author_sort Zhang, Miaomiao
collection PubMed
description Mature biofilm formation by Vibrio parahaemolyticus requires exopolysaccharide (EPS), type IV pili, and capsular polysaccharide (CPS). Production of each is strictly regulated by various control pathways including quorum sensing (QS) and bis-(3′–5′)-cyclic di-GMP (c-di-GMP). QsvR, an AraC-type regulator, integrates into the QS regulatory cascade via direct control of the transcription of the master QS regulators, AphA and OpaR. Deletion of qsvR in wild-type or opaR mutant backgrounds altered the biofilm formation by V. parahaemolyticus, suggesting that QsvR may coordinate with OpaR to control biofilm formation. Herein, we demonstrated both QsvR and OpaR repressed biofilm-associated phenotypes, c-di-GMP metabolism, and the formation of V. parahaemolyticus translucent (TR) colonies. QsvR restored the biofilm-associated phenotypic changes caused by opaR mutation, and vice versa. In addition, QsvR and OpaR worked coordinately to regulate the transcription of EPS-associated genes, type IV pili genes, CPS genes and c-di-GMP metabolism-related genes. These results demonstrated how QsvR works with the QS system to regulate biofilm formation by precisely controlling the transcription of multiple biofilm formation-associated genes in V. parahaemolyticus.
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spelling pubmed-99487392023-02-24 QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus Zhang, Miaomiao Xue, Xingfan Li, Xue Wu, Qimin Zhang, Tingting Yang, Wenhui Hu, Lingfei Zhou, Dongsheng Lu, Renfei Zhang, Yiquan Front Microbiol Microbiology Mature biofilm formation by Vibrio parahaemolyticus requires exopolysaccharide (EPS), type IV pili, and capsular polysaccharide (CPS). Production of each is strictly regulated by various control pathways including quorum sensing (QS) and bis-(3′–5′)-cyclic di-GMP (c-di-GMP). QsvR, an AraC-type regulator, integrates into the QS regulatory cascade via direct control of the transcription of the master QS regulators, AphA and OpaR. Deletion of qsvR in wild-type or opaR mutant backgrounds altered the biofilm formation by V. parahaemolyticus, suggesting that QsvR may coordinate with OpaR to control biofilm formation. Herein, we demonstrated both QsvR and OpaR repressed biofilm-associated phenotypes, c-di-GMP metabolism, and the formation of V. parahaemolyticus translucent (TR) colonies. QsvR restored the biofilm-associated phenotypic changes caused by opaR mutation, and vice versa. In addition, QsvR and OpaR worked coordinately to regulate the transcription of EPS-associated genes, type IV pili genes, CPS genes and c-di-GMP metabolism-related genes. These results demonstrated how QsvR works with the QS system to regulate biofilm formation by precisely controlling the transcription of multiple biofilm formation-associated genes in V. parahaemolyticus. Frontiers Media S.A. 2023-02-09 /pmc/articles/PMC9948739/ /pubmed/36846774 http://dx.doi.org/10.3389/fmicb.2023.1079653 Text en Copyright © 2023 Zhang, Xue, Li, Wu, Zhang, Yang, Hu, Zhou, Lu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Miaomiao
Xue, Xingfan
Li, Xue
Wu, Qimin
Zhang, Tingting
Yang, Wenhui
Hu, Lingfei
Zhou, Dongsheng
Lu, Renfei
Zhang, Yiquan
QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
title QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
title_full QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
title_fullStr QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
title_full_unstemmed QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
title_short QsvR and OpaR coordinately repress biofilm formation by Vibrio parahaemolyticus
title_sort qsvr and opar coordinately repress biofilm formation by vibrio parahaemolyticus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9948739/
https://www.ncbi.nlm.nih.gov/pubmed/36846774
http://dx.doi.org/10.3389/fmicb.2023.1079653
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