Cargando…

LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D

The incidence and mortality of cervical cancer (CC) rank fourth among those of all gynecological malignancies. Long noncoding RNAs (lncRNAs) serve important roles in the development of various types of cancer. The aim of the present study was to explore the role of lncRNAs in the pathogenesis of CC...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Keyi, Ni, Xiao, Ma, Xiaoling, Sun, Rui, Qiu, Jiangnan, Luo, Chengyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9950337/
https://www.ncbi.nlm.nih.gov/pubmed/36844616
http://dx.doi.org/10.3892/ol.2023.13710
_version_ 1784893140911521792
author Zhang, Keyi
Ni, Xiao
Ma, Xiaoling
Sun, Rui
Qiu, Jiangnan
Luo, Chengyan
author_facet Zhang, Keyi
Ni, Xiao
Ma, Xiaoling
Sun, Rui
Qiu, Jiangnan
Luo, Chengyan
author_sort Zhang, Keyi
collection PubMed
description The incidence and mortality of cervical cancer (CC) rank fourth among those of all gynecological malignancies. Long noncoding RNAs (lncRNAs) serve important roles in the development of various types of cancer. The aim of the present study was to explore the role of lncRNAs in the pathogenesis of CC and to identify novel therapeutic targets. LINC01012 was identified to be associated with an unfavorable prognosis in patients with CC based on bioinformatics analyses. Upregulated LINC01012 expression was further verified in CC samples and in cervical intraepithelial neoplasia grade 3 tissues compared with healthy tissues using reverse transcription-quantitative PCR. Functionally, following transfection with LINC01012 short hairpin RNA (sh-LINC01012), the proliferation and migration of CC cell lines were examined using 5-ethynyl-2′-deoxyuridine staining, colony formation and Transwell assays, which demonstrated that knockdown of LINC01012 in CC cells suppressed cell proliferation and migration in vitro and tumor growth in an in vivo xenograft model. The potential mechanisms of LINC01012 were further explored. A negative association between LINC01012 and cyclin dependent kinase inhibitor 2D (CDKN2D) was also identified based on The Cancer Genome Atlas data and this was confirmed using western blotting and rescue experiments. Consistently, knockdown of LINC01012 in CC cells upregulated CDKN2D expression. The inhibition of proliferation and migration of CC cells following transfection with sh-LINC01012 was reversed following co-transfection of sh-LINC01012 and CDKN2D short hairpin RNA. These findings suggested that upregulated LINC01012 expression in CC may stimulate the proliferation and migration of cancer cells, thus promoting CC progression via downregulation of CDKN2D.
format Online
Article
Text
id pubmed-9950337
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-99503372023-02-25 LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D Zhang, Keyi Ni, Xiao Ma, Xiaoling Sun, Rui Qiu, Jiangnan Luo, Chengyan Oncol Lett Articles The incidence and mortality of cervical cancer (CC) rank fourth among those of all gynecological malignancies. Long noncoding RNAs (lncRNAs) serve important roles in the development of various types of cancer. The aim of the present study was to explore the role of lncRNAs in the pathogenesis of CC and to identify novel therapeutic targets. LINC01012 was identified to be associated with an unfavorable prognosis in patients with CC based on bioinformatics analyses. Upregulated LINC01012 expression was further verified in CC samples and in cervical intraepithelial neoplasia grade 3 tissues compared with healthy tissues using reverse transcription-quantitative PCR. Functionally, following transfection with LINC01012 short hairpin RNA (sh-LINC01012), the proliferation and migration of CC cell lines were examined using 5-ethynyl-2′-deoxyuridine staining, colony formation and Transwell assays, which demonstrated that knockdown of LINC01012 in CC cells suppressed cell proliferation and migration in vitro and tumor growth in an in vivo xenograft model. The potential mechanisms of LINC01012 were further explored. A negative association between LINC01012 and cyclin dependent kinase inhibitor 2D (CDKN2D) was also identified based on The Cancer Genome Atlas data and this was confirmed using western blotting and rescue experiments. Consistently, knockdown of LINC01012 in CC cells upregulated CDKN2D expression. The inhibition of proliferation and migration of CC cells following transfection with sh-LINC01012 was reversed following co-transfection of sh-LINC01012 and CDKN2D short hairpin RNA. These findings suggested that upregulated LINC01012 expression in CC may stimulate the proliferation and migration of cancer cells, thus promoting CC progression via downregulation of CDKN2D. D.A. Spandidos 2023-02-08 /pmc/articles/PMC9950337/ /pubmed/36844616 http://dx.doi.org/10.3892/ol.2023.13710 Text en Copyright: © Zhang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Keyi
Ni, Xiao
Ma, Xiaoling
Sun, Rui
Qiu, Jiangnan
Luo, Chengyan
LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D
title LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D
title_full LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D
title_fullStr LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D
title_full_unstemmed LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D
title_short LINC01012 upregulation promotes cervical cancer proliferation and migration via downregulation of CDKN2D
title_sort linc01012 upregulation promotes cervical cancer proliferation and migration via downregulation of cdkn2d
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9950337/
https://www.ncbi.nlm.nih.gov/pubmed/36844616
http://dx.doi.org/10.3892/ol.2023.13710
work_keys_str_mv AT zhangkeyi linc01012upregulationpromotescervicalcancerproliferationandmigrationviadownregulationofcdkn2d
AT nixiao linc01012upregulationpromotescervicalcancerproliferationandmigrationviadownregulationofcdkn2d
AT maxiaoling linc01012upregulationpromotescervicalcancerproliferationandmigrationviadownregulationofcdkn2d
AT sunrui linc01012upregulationpromotescervicalcancerproliferationandmigrationviadownregulationofcdkn2d
AT qiujiangnan linc01012upregulationpromotescervicalcancerproliferationandmigrationviadownregulationofcdkn2d
AT luochengyan linc01012upregulationpromotescervicalcancerproliferationandmigrationviadownregulationofcdkn2d