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Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study

OBJECTIVE: Positive human epidermal growth factor 2 (HER2) expression and its predictive clinicopathological features remain unclear in Sri Lankan gastric cancer (GC) patients. Here, we aimed to determine GC HER2 status predictors by analyzing associations between clinicopathological features and HE...

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Autores principales: Subasinghe, Duminda, Acott, Nathan, Mahesh, Pasyodun Koralage Buddhika, Sivaganesh, Sivasuriya, Munasinghe, Sithum, Kumarasinghe, Mariyan Priyanthi, Samarasekera, Dharmabandu Nandaveva, Lokuhetty, Menaka Dilani Samarawickrema
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9950620/
https://www.ncbi.nlm.nih.gov/pubmed/36814374
http://dx.doi.org/10.1177/03000605231154403
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author Subasinghe, Duminda
Acott, Nathan
Mahesh, Pasyodun Koralage Buddhika
Sivaganesh, Sivasuriya
Munasinghe, Sithum
Kumarasinghe, Mariyan Priyanthi
Samarasekera, Dharmabandu Nandaveva
Lokuhetty, Menaka Dilani Samarawickrema
author_facet Subasinghe, Duminda
Acott, Nathan
Mahesh, Pasyodun Koralage Buddhika
Sivaganesh, Sivasuriya
Munasinghe, Sithum
Kumarasinghe, Mariyan Priyanthi
Samarasekera, Dharmabandu Nandaveva
Lokuhetty, Menaka Dilani Samarawickrema
author_sort Subasinghe, Duminda
collection PubMed
description OBJECTIVE: Positive human epidermal growth factor 2 (HER2) expression and its predictive clinicopathological features remain unclear in Sri Lankan gastric cancer (GC) patients. Here, we aimed to determine GC HER2 status predictors by analyzing associations between clinicopathological features and HER2 expression using immunohistochemistry (IHC) and silver in situ hybridization (SISH). METHODS: During this 4-year prospective study, clinicopathological data were collected from participants in the National Hospital of Sri Lanka. HER2 IHC and SISH were performed using commercial reagents. Using chi-square tests, associations of HER2-IHC/SISH with clinicopathological features were analyzed. RESULTS: Overall, 145 GC patients were included, 69 had gastrectomies and 76 had biopsies. Positive HER2 expression by IHC was associated with age <60 years, high T stage (assessed pathologically in resections and radiologically in biopsies), high nuclear grade, tumor necrosis, mitosis >5/high-power field, with additional perineural invasion and lymphovascular invasion in resections. These features, excluding lymphovascular invasion but including male sex, were associated with HER2 expression by SISH. CONCLUSIONS: Age <60 years, high nuclear grade, tumor necrosis, and perineural invasion are associated factors of HER2 status. These could be used to triage GC patients for HER2 status testing in limited resource settings where IHC/SISH analysis is costly.
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spelling pubmed-99506202023-02-25 Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study Subasinghe, Duminda Acott, Nathan Mahesh, Pasyodun Koralage Buddhika Sivaganesh, Sivasuriya Munasinghe, Sithum Kumarasinghe, Mariyan Priyanthi Samarasekera, Dharmabandu Nandaveva Lokuhetty, Menaka Dilani Samarawickrema J Int Med Res Prospective Clinical Research Report OBJECTIVE: Positive human epidermal growth factor 2 (HER2) expression and its predictive clinicopathological features remain unclear in Sri Lankan gastric cancer (GC) patients. Here, we aimed to determine GC HER2 status predictors by analyzing associations between clinicopathological features and HER2 expression using immunohistochemistry (IHC) and silver in situ hybridization (SISH). METHODS: During this 4-year prospective study, clinicopathological data were collected from participants in the National Hospital of Sri Lanka. HER2 IHC and SISH were performed using commercial reagents. Using chi-square tests, associations of HER2-IHC/SISH with clinicopathological features were analyzed. RESULTS: Overall, 145 GC patients were included, 69 had gastrectomies and 76 had biopsies. Positive HER2 expression by IHC was associated with age <60 years, high T stage (assessed pathologically in resections and radiologically in biopsies), high nuclear grade, tumor necrosis, mitosis >5/high-power field, with additional perineural invasion and lymphovascular invasion in resections. These features, excluding lymphovascular invasion but including male sex, were associated with HER2 expression by SISH. CONCLUSIONS: Age <60 years, high nuclear grade, tumor necrosis, and perineural invasion are associated factors of HER2 status. These could be used to triage GC patients for HER2 status testing in limited resource settings where IHC/SISH analysis is costly. SAGE Publications 2023-02-22 /pmc/articles/PMC9950620/ /pubmed/36814374 http://dx.doi.org/10.1177/03000605231154403 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Prospective Clinical Research Report
Subasinghe, Duminda
Acott, Nathan
Mahesh, Pasyodun Koralage Buddhika
Sivaganesh, Sivasuriya
Munasinghe, Sithum
Kumarasinghe, Mariyan Priyanthi
Samarasekera, Dharmabandu Nandaveva
Lokuhetty, Menaka Dilani Samarawickrema
Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study
title Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study
title_full Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study
title_fullStr Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study
title_full_unstemmed Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study
title_short Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study
title_sort human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of sri lankan patients with gastric adenocarcinoma: a prospective study
topic Prospective Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9950620/
https://www.ncbi.nlm.nih.gov/pubmed/36814374
http://dx.doi.org/10.1177/03000605231154403
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