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Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study
OBJECTIVE: Positive human epidermal growth factor 2 (HER2) expression and its predictive clinicopathological features remain unclear in Sri Lankan gastric cancer (GC) patients. Here, we aimed to determine GC HER2 status predictors by analyzing associations between clinicopathological features and HE...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9950620/ https://www.ncbi.nlm.nih.gov/pubmed/36814374 http://dx.doi.org/10.1177/03000605231154403 |
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author | Subasinghe, Duminda Acott, Nathan Mahesh, Pasyodun Koralage Buddhika Sivaganesh, Sivasuriya Munasinghe, Sithum Kumarasinghe, Mariyan Priyanthi Samarasekera, Dharmabandu Nandaveva Lokuhetty, Menaka Dilani Samarawickrema |
author_facet | Subasinghe, Duminda Acott, Nathan Mahesh, Pasyodun Koralage Buddhika Sivaganesh, Sivasuriya Munasinghe, Sithum Kumarasinghe, Mariyan Priyanthi Samarasekera, Dharmabandu Nandaveva Lokuhetty, Menaka Dilani Samarawickrema |
author_sort | Subasinghe, Duminda |
collection | PubMed |
description | OBJECTIVE: Positive human epidermal growth factor 2 (HER2) expression and its predictive clinicopathological features remain unclear in Sri Lankan gastric cancer (GC) patients. Here, we aimed to determine GC HER2 status predictors by analyzing associations between clinicopathological features and HER2 expression using immunohistochemistry (IHC) and silver in situ hybridization (SISH). METHODS: During this 4-year prospective study, clinicopathological data were collected from participants in the National Hospital of Sri Lanka. HER2 IHC and SISH were performed using commercial reagents. Using chi-square tests, associations of HER2-IHC/SISH with clinicopathological features were analyzed. RESULTS: Overall, 145 GC patients were included, 69 had gastrectomies and 76 had biopsies. Positive HER2 expression by IHC was associated with age <60 years, high T stage (assessed pathologically in resections and radiologically in biopsies), high nuclear grade, tumor necrosis, mitosis >5/high-power field, with additional perineural invasion and lymphovascular invasion in resections. These features, excluding lymphovascular invasion but including male sex, were associated with HER2 expression by SISH. CONCLUSIONS: Age <60 years, high nuclear grade, tumor necrosis, and perineural invasion are associated factors of HER2 status. These could be used to triage GC patients for HER2 status testing in limited resource settings where IHC/SISH analysis is costly. |
format | Online Article Text |
id | pubmed-9950620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-99506202023-02-25 Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study Subasinghe, Duminda Acott, Nathan Mahesh, Pasyodun Koralage Buddhika Sivaganesh, Sivasuriya Munasinghe, Sithum Kumarasinghe, Mariyan Priyanthi Samarasekera, Dharmabandu Nandaveva Lokuhetty, Menaka Dilani Samarawickrema J Int Med Res Prospective Clinical Research Report OBJECTIVE: Positive human epidermal growth factor 2 (HER2) expression and its predictive clinicopathological features remain unclear in Sri Lankan gastric cancer (GC) patients. Here, we aimed to determine GC HER2 status predictors by analyzing associations between clinicopathological features and HER2 expression using immunohistochemistry (IHC) and silver in situ hybridization (SISH). METHODS: During this 4-year prospective study, clinicopathological data were collected from participants in the National Hospital of Sri Lanka. HER2 IHC and SISH were performed using commercial reagents. Using chi-square tests, associations of HER2-IHC/SISH with clinicopathological features were analyzed. RESULTS: Overall, 145 GC patients were included, 69 had gastrectomies and 76 had biopsies. Positive HER2 expression by IHC was associated with age <60 years, high T stage (assessed pathologically in resections and radiologically in biopsies), high nuclear grade, tumor necrosis, mitosis >5/high-power field, with additional perineural invasion and lymphovascular invasion in resections. These features, excluding lymphovascular invasion but including male sex, were associated with HER2 expression by SISH. CONCLUSIONS: Age <60 years, high nuclear grade, tumor necrosis, and perineural invasion are associated factors of HER2 status. These could be used to triage GC patients for HER2 status testing in limited resource settings where IHC/SISH analysis is costly. SAGE Publications 2023-02-22 /pmc/articles/PMC9950620/ /pubmed/36814374 http://dx.doi.org/10.1177/03000605231154403 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Prospective Clinical Research Report Subasinghe, Duminda Acott, Nathan Mahesh, Pasyodun Koralage Buddhika Sivaganesh, Sivasuriya Munasinghe, Sithum Kumarasinghe, Mariyan Priyanthi Samarasekera, Dharmabandu Nandaveva Lokuhetty, Menaka Dilani Samarawickrema Human epidermal growth factor receptor-2 gene expression positivity determined by silver in situ hybridization/immunohistochemistry methods and associated factors in a cohort of Sri Lankan patients with gastric adenocarcinoma: a prospective study |
title | Human epidermal growth factor receptor-2 gene expression positivity
determined by silver in situ hybridization/immunohistochemistry methods and
associated factors in a cohort of Sri Lankan patients with gastric
adenocarcinoma: a prospective study |
title_full | Human epidermal growth factor receptor-2 gene expression positivity
determined by silver in situ hybridization/immunohistochemistry methods and
associated factors in a cohort of Sri Lankan patients with gastric
adenocarcinoma: a prospective study |
title_fullStr | Human epidermal growth factor receptor-2 gene expression positivity
determined by silver in situ hybridization/immunohistochemistry methods and
associated factors in a cohort of Sri Lankan patients with gastric
adenocarcinoma: a prospective study |
title_full_unstemmed | Human epidermal growth factor receptor-2 gene expression positivity
determined by silver in situ hybridization/immunohistochemistry methods and
associated factors in a cohort of Sri Lankan patients with gastric
adenocarcinoma: a prospective study |
title_short | Human epidermal growth factor receptor-2 gene expression positivity
determined by silver in situ hybridization/immunohistochemistry methods and
associated factors in a cohort of Sri Lankan patients with gastric
adenocarcinoma: a prospective study |
title_sort | human epidermal growth factor receptor-2 gene expression positivity
determined by silver in situ hybridization/immunohistochemistry methods and
associated factors in a cohort of sri lankan patients with gastric
adenocarcinoma: a prospective study |
topic | Prospective Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9950620/ https://www.ncbi.nlm.nih.gov/pubmed/36814374 http://dx.doi.org/10.1177/03000605231154403 |
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