False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York

The presence of hemoglobin (Hb) variants might interfere with some glycated hemoglobin (HbA(1c)) measurements. There have been a few reports of compound Hb variants affecting HbA(1c) testing. Here, we report a case of the coinheritance of two Hb variants in the β‐globin gene. High‐performance liquid...

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Detalles Bibliográficos
Autores principales: Li, Youqiong, Tang, Jianling, Zheng, Lihong, Tang, Kaijian, Li, Lingli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9951559/
https://www.ncbi.nlm.nih.gov/pubmed/36625083
http://dx.doi.org/10.1111/jdi.13971
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author Li, Youqiong
Tang, Jianling
Zheng, Lihong
Tang, Kaijian
Li, Lingli
author_facet Li, Youqiong
Tang, Jianling
Zheng, Lihong
Tang, Kaijian
Li, Lingli
author_sort Li, Youqiong
collection PubMed
description The presence of hemoglobin (Hb) variants might interfere with some glycated hemoglobin (HbA(1c)) measurements. There have been a few reports of compound Hb variants affecting HbA(1c) testing. Here, we report a case of the coinheritance of two Hb variants in the β‐globin gene. High‐performance liquid chromatography with the Hb program showed a high HbA(2) level. Similarly, an E‐window peak was separated on the high‐performance liquid chromatography with a glycated Hb program. However, capillary electrophoresis showed two abnormal peaks and no HbA peak. Sanger sequencing confirmed the presence of Hb New York and HbE. This is the first report of a compound heterozygote for HbE and Hb New York. The double heterozygote caused erroneous results for HbA(1c) on high‐performance liquid chromatography and enzyme assay.
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spelling pubmed-99515592023-02-25 False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York Li, Youqiong Tang, Jianling Zheng, Lihong Tang, Kaijian Li, Lingli J Diabetes Investig Articles The presence of hemoglobin (Hb) variants might interfere with some glycated hemoglobin (HbA(1c)) measurements. There have been a few reports of compound Hb variants affecting HbA(1c) testing. Here, we report a case of the coinheritance of two Hb variants in the β‐globin gene. High‐performance liquid chromatography with the Hb program showed a high HbA(2) level. Similarly, an E‐window peak was separated on the high‐performance liquid chromatography with a glycated Hb program. However, capillary electrophoresis showed two abnormal peaks and no HbA peak. Sanger sequencing confirmed the presence of Hb New York and HbE. This is the first report of a compound heterozygote for HbE and Hb New York. The double heterozygote caused erroneous results for HbA(1c) on high‐performance liquid chromatography and enzyme assay. John Wiley and Sons Inc. 2023-01-10 /pmc/articles/PMC9951559/ /pubmed/36625083 http://dx.doi.org/10.1111/jdi.13971 Text en © 2023 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Articles
Li, Youqiong
Tang, Jianling
Zheng, Lihong
Tang, Kaijian
Li, Lingli
False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York
title False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York
title_full False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York
title_fullStr False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York
title_full_unstemmed False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York
title_short False hemoglobin A(1c) value as a result of compound heterozygotes of hemoglobin E and hemoglobin New York
title_sort false hemoglobin a(1c) value as a result of compound heterozygotes of hemoglobin e and hemoglobin new york
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9951559/
https://www.ncbi.nlm.nih.gov/pubmed/36625083
http://dx.doi.org/10.1111/jdi.13971
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