Serum Oxytocin, Cortisol and Social Behavior in Calves: A Study in the Impossible Task Paradigm

SIMPLE SUMMARY: We investigated the relationship between circulating levels of the hormones oxytocin and cortisol and some behaviors directed at humans by calves in the impossible task paradigm. Cortisol correlates positively with the latency of behaviors directed at the caregiver and the duration of behaviors directed at the apparatus correlates negatively with people. Contrary to what is reported in the literature on cows, no correlations were found between oxytocin levels and direct behaviors toward the caregiver. This highlights a different behavioral strategy between calves and cows when faced with solving an impossible task. ABSTRACT: In this study, we explored the correlations between circulating levels of oxytocin, cortisol, and different social behaviors toward humans in 26 Italian Red Pied calves (all females, with an average age of 174 ± 24 days) using the impossible task paradigm. This paradigm has proved fruitful in highlighting the effect of socialization on the willingness to interact with humans in several domesticated species. The test consists of the violation of an expectation (recovering food from an experimental apparatus) while a caregiver and a stranger are present. Immediately after the end of the test (less than one minute), blood was collected from the coccygeal vein. Statistics were performed by the Spearman’s rank correlation; significant differences were adjusted according to Bonferroni’s correction. Cortisol correlates positively (ρ = 0.565; p < 0.05) with the latency of behaviors directed at the caregiver, and the duration of behaviors directed at the apparatus correlates negatively with both the caregiver (ρ = −0.654; p < 0.05) and a stranger (ρ = −0.644; p < 0.05). Contrary to what is reported in the literature on cows, no correlations were found between oxytocin levels and direct behaviors toward the caregiver. This highlights a different behavioral strategy between calves and cows when placed in front of an impossible task.