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In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats

BACKGROUND AND PURPOSE: The purpose of the current study was to investigate the protective effects of acute and chronic administration of different doses of vitamin D3 on pentylenetetrazol (PTZ)-induced epileptiform activities in rats. EXPERIMENTAL APPROACH: Sixty Wistar rats in chronic and acute gr...

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Autores principales: Gharehaghaji, Sara Hosseinchi, Panahi, Yousef, Khalilzadeh, Emad, Saiah, Gholamreza Vafaei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9951785/
https://www.ncbi.nlm.nih.gov/pubmed/36846737
http://dx.doi.org/10.4103/1735-5362.363596
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author Gharehaghaji, Sara Hosseinchi
Panahi, Yousef
Khalilzadeh, Emad
Saiah, Gholamreza Vafaei
author_facet Gharehaghaji, Sara Hosseinchi
Panahi, Yousef
Khalilzadeh, Emad
Saiah, Gholamreza Vafaei
author_sort Gharehaghaji, Sara Hosseinchi
collection PubMed
description BACKGROUND AND PURPOSE: The purpose of the current study was to investigate the protective effects of acute and chronic administration of different doses of vitamin D3 on pentylenetetrazol (PTZ)-induced epileptiform activities in rats. EXPERIMENTAL APPROACH: Sixty Wistar rats in chronic and acute groups were used in this study. In the chronic groups, animals received vitamin D3 at 50, 100, and 150 μg/kg; vitamin D3 (50 μg/kg, i.p.) + diazepam (0.1 mg/kg, i.p.), and almond oil (i.p.) daily for two weeks whereas, in the acute groups the animal received a single dose of chemicals just 30 min before PTZ administration. The electrophysiological recording was performed by implanting a unilateral bipolar electrode in the pyramidal cell layer of the CA1 region of the hippocampus. Epileptic activities were induced by intraperitoneal injection of PTZ (80 mg/kg, i.p.). The spike count and amplitude were analyzed using the eTrace software. FINDING/RESULTS: Chronic administration of all doses of vitamin D3 and its combination with diazepam significantly reduced both spike counts and amplitudes following PTZ administration. While the acute doses were ineffective. CONCLUSION AND IMPLICATION: The results of the study indicated that chronic but not acute administration of vitamin D3 has a protective effect on PTZ-induced epileptiform activity in rats.
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spelling pubmed-99517852023-02-25 In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats Gharehaghaji, Sara Hosseinchi Panahi, Yousef Khalilzadeh, Emad Saiah, Gholamreza Vafaei Res Pharm Sci Original Article BACKGROUND AND PURPOSE: The purpose of the current study was to investigate the protective effects of acute and chronic administration of different doses of vitamin D3 on pentylenetetrazol (PTZ)-induced epileptiform activities in rats. EXPERIMENTAL APPROACH: Sixty Wistar rats in chronic and acute groups were used in this study. In the chronic groups, animals received vitamin D3 at 50, 100, and 150 μg/kg; vitamin D3 (50 μg/kg, i.p.) + diazepam (0.1 mg/kg, i.p.), and almond oil (i.p.) daily for two weeks whereas, in the acute groups the animal received a single dose of chemicals just 30 min before PTZ administration. The electrophysiological recording was performed by implanting a unilateral bipolar electrode in the pyramidal cell layer of the CA1 region of the hippocampus. Epileptic activities were induced by intraperitoneal injection of PTZ (80 mg/kg, i.p.). The spike count and amplitude were analyzed using the eTrace software. FINDING/RESULTS: Chronic administration of all doses of vitamin D3 and its combination with diazepam significantly reduced both spike counts and amplitudes following PTZ administration. While the acute doses were ineffective. CONCLUSION AND IMPLICATION: The results of the study indicated that chronic but not acute administration of vitamin D3 has a protective effect on PTZ-induced epileptiform activity in rats. Wolters Kluwer - Medknow 2022-12-24 /pmc/articles/PMC9951785/ /pubmed/36846737 http://dx.doi.org/10.4103/1735-5362.363596 Text en Copyright: © 2022 Research in Pharmaceutical Sciences https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Gharehaghaji, Sara Hosseinchi
Panahi, Yousef
Khalilzadeh, Emad
Saiah, Gholamreza Vafaei
In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats
title In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats
title_full In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats
title_fullStr In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats
title_full_unstemmed In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats
title_short In vivo electrophysiological study of vitamin D3 protective effects on PTZ-induced seizures in rats
title_sort in vivo electrophysiological study of vitamin d3 protective effects on ptz-induced seizures in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9951785/
https://www.ncbi.nlm.nih.gov/pubmed/36846737
http://dx.doi.org/10.4103/1735-5362.363596
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