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Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome
The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well docum...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9951915/ https://www.ncbi.nlm.nih.gov/pubmed/36830192 http://dx.doi.org/10.3390/antibiotics12020281 |
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author | Sánchez-Osuna, Miquel Barbé, Jordi Erill, Ivan |
author_facet | Sánchez-Osuna, Miquel Barbé, Jordi Erill, Ivan |
author_sort | Sánchez-Osuna, Miquel |
collection | PubMed |
description | The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well documented, but few studies have analyzed the patterns underpinning the dissemination of antimicrobial resistance genes. Analyzing the %GC content of plasmid-borne antimicrobial resistance genes relative to their host genome %GC content provides a means to efficiently detect and quantify dissemination of antimicrobial resistance genes. In this work we automate %GC content analysis to perform a comprehensive analysis of known antimicrobial resistance genes in publicly available plasmid sequences. We find that the degree to which antimicrobial resistance genes are disseminated depends primarily on the resistance mechanism. Our analysis identifies conjugative plasmids as primary dissemination vectors and indicates that most broadly disseminated genes have spread from single genomic backgrounds. We show that resistance dissemination profiles vary greatly among antimicrobials, oftentimes reflecting stewardship measures. Our findings establish %GC content analysis as a powerful, intuitive and scalable method to monitor the dissemination of resistance determinants using publicly available sequence data. |
format | Online Article Text |
id | pubmed-9951915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99519152023-02-25 Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome Sánchez-Osuna, Miquel Barbé, Jordi Erill, Ivan Antibiotics (Basel) Article The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well documented, but few studies have analyzed the patterns underpinning the dissemination of antimicrobial resistance genes. Analyzing the %GC content of plasmid-borne antimicrobial resistance genes relative to their host genome %GC content provides a means to efficiently detect and quantify dissemination of antimicrobial resistance genes. In this work we automate %GC content analysis to perform a comprehensive analysis of known antimicrobial resistance genes in publicly available plasmid sequences. We find that the degree to which antimicrobial resistance genes are disseminated depends primarily on the resistance mechanism. Our analysis identifies conjugative plasmids as primary dissemination vectors and indicates that most broadly disseminated genes have spread from single genomic backgrounds. We show that resistance dissemination profiles vary greatly among antimicrobials, oftentimes reflecting stewardship measures. Our findings establish %GC content analysis as a powerful, intuitive and scalable method to monitor the dissemination of resistance determinants using publicly available sequence data. MDPI 2023-02-01 /pmc/articles/PMC9951915/ /pubmed/36830192 http://dx.doi.org/10.3390/antibiotics12020281 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sánchez-Osuna, Miquel Barbé, Jordi Erill, Ivan Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome |
title | Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome |
title_full | Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome |
title_fullStr | Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome |
title_full_unstemmed | Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome |
title_short | Systematic In Silico Assessment of Antimicrobial Resistance Dissemination across the Global Plasmidome |
title_sort | systematic in silico assessment of antimicrobial resistance dissemination across the global plasmidome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9951915/ https://www.ncbi.nlm.nih.gov/pubmed/36830192 http://dx.doi.org/10.3390/antibiotics12020281 |
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