Cargando…

Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement

To enable rapid osteointegration in bioceramic implants and to give them osteoinductive properties, scaffolds with defined micro- and macroporosity are required. Pores or pore networks promote the integration of cells into the implant, facilitating the supply of nutrients and the removal of metaboli...

Descripción completa

Detalles Bibliográficos
Autores principales: Dufner, Lukas, Oßwald, Bettina, Eberspaecher, Jan, Riedel, Bianca, Kling, Chiara, Kern, Frank, Seidenstuecker, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952018/
https://www.ncbi.nlm.nih.gov/pubmed/36829750
http://dx.doi.org/10.3390/bioengineering10020256
_version_ 1784893525491449856
author Dufner, Lukas
Oßwald, Bettina
Eberspaecher, Jan
Riedel, Bianca
Kling, Chiara
Kern, Frank
Seidenstuecker, Michael
author_facet Dufner, Lukas
Oßwald, Bettina
Eberspaecher, Jan
Riedel, Bianca
Kling, Chiara
Kern, Frank
Seidenstuecker, Michael
author_sort Dufner, Lukas
collection PubMed
description To enable rapid osteointegration in bioceramic implants and to give them osteoinductive properties, scaffolds with defined micro- and macroporosity are required. Pores or pore networks promote the integration of cells into the implant, facilitating the supply of nutrients and the removal of metabolic products. In this paper, scaffolds are created from ß-tricalciumphosphate (ß-TCP) and in a novel way, where both the micro- and macroporosity are adjusted simultaneously by the addition of pore-forming polymer particles. The particles used are 10–40 wt%, spherical polymer particles of polymethylmethacrylate (PMMA) (Ø = 5 µm) and alternatively polymethylsilsesquioxane (PMSQ) (Ø = 2 µm), added in the course of ß-TCP slurry preparation. The arrangement of hydrophobic polymer particles at the interface of air bubbles was incorporated during slurry preparation and foaming of the slurry. The foam structures remain after sintering and lead to the formation of macro-porosity in the scaffolds. Furthermore, decomposition of the polymer particles during thermal debindering results in the formation of an additional network of interconnecting micropores in the stabilizing structures. It is possible to adjust the porosity easily and quickly in a range of 1.2–140 μm with a relatively low organic fraction. The structures thus prepared showed no cytotoxicity nor negative effects on the biocompatibility.
format Online
Article
Text
id pubmed-9952018
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99520182023-02-25 Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement Dufner, Lukas Oßwald, Bettina Eberspaecher, Jan Riedel, Bianca Kling, Chiara Kern, Frank Seidenstuecker, Michael Bioengineering (Basel) Article To enable rapid osteointegration in bioceramic implants and to give them osteoinductive properties, scaffolds with defined micro- and macroporosity are required. Pores or pore networks promote the integration of cells into the implant, facilitating the supply of nutrients and the removal of metabolic products. In this paper, scaffolds are created from ß-tricalciumphosphate (ß-TCP) and in a novel way, where both the micro- and macroporosity are adjusted simultaneously by the addition of pore-forming polymer particles. The particles used are 10–40 wt%, spherical polymer particles of polymethylmethacrylate (PMMA) (Ø = 5 µm) and alternatively polymethylsilsesquioxane (PMSQ) (Ø = 2 µm), added in the course of ß-TCP slurry preparation. The arrangement of hydrophobic polymer particles at the interface of air bubbles was incorporated during slurry preparation and foaming of the slurry. The foam structures remain after sintering and lead to the formation of macro-porosity in the scaffolds. Furthermore, decomposition of the polymer particles during thermal debindering results in the formation of an additional network of interconnecting micropores in the stabilizing structures. It is possible to adjust the porosity easily and quickly in a range of 1.2–140 μm with a relatively low organic fraction. The structures thus prepared showed no cytotoxicity nor negative effects on the biocompatibility. MDPI 2023-02-15 /pmc/articles/PMC9952018/ /pubmed/36829750 http://dx.doi.org/10.3390/bioengineering10020256 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dufner, Lukas
Oßwald, Bettina
Eberspaecher, Jan
Riedel, Bianca
Kling, Chiara
Kern, Frank
Seidenstuecker, Michael
Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement
title Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement
title_full Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement
title_fullStr Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement
title_full_unstemmed Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement
title_short Adjustment of Micro- and Macroporosity of ß-TCP Scaffolds Using Solid-Stabilized Foams as Bone Replacement
title_sort adjustment of micro- and macroporosity of ß-tcp scaffolds using solid-stabilized foams as bone replacement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952018/
https://www.ncbi.nlm.nih.gov/pubmed/36829750
http://dx.doi.org/10.3390/bioengineering10020256
work_keys_str_mv AT dufnerlukas adjustmentofmicroandmacroporosityofßtcpscaffoldsusingsolidstabilizedfoamsasbonereplacement
AT oßwaldbettina adjustmentofmicroandmacroporosityofßtcpscaffoldsusingsolidstabilizedfoamsasbonereplacement
AT eberspaecherjan adjustmentofmicroandmacroporosityofßtcpscaffoldsusingsolidstabilizedfoamsasbonereplacement
AT riedelbianca adjustmentofmicroandmacroporosityofßtcpscaffoldsusingsolidstabilizedfoamsasbonereplacement
AT klingchiara adjustmentofmicroandmacroporosityofßtcpscaffoldsusingsolidstabilizedfoamsasbonereplacement
AT kernfrank adjustmentofmicroandmacroporosityofßtcpscaffoldsusingsolidstabilizedfoamsasbonereplacement
AT seidenstueckermichael adjustmentofmicroandmacroporosityofßtcpscaffoldsusingsolidstabilizedfoamsasbonereplacement