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Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis

Antimicrobial resistance is a serious problem for the control of infections and infectious diseases. Propolis is a substance produced by honeybees with antimicrobial and antibiofilm properties. To consider propolis as an alternative to the use of antimicrobials for infection control, we assessed its...

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Autores principales: Queiroga, Maria Cristina, Laranjo, Marta, Andrade, Nara, Marques, Mariana, Costa, Ana Rodrigues, Antunes, Célia Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952062/
https://www.ncbi.nlm.nih.gov/pubmed/36830258
http://dx.doi.org/10.3390/antibiotics12020347
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author Queiroga, Maria Cristina
Laranjo, Marta
Andrade, Nara
Marques, Mariana
Costa, Ana Rodrigues
Antunes, Célia Maria
author_facet Queiroga, Maria Cristina
Laranjo, Marta
Andrade, Nara
Marques, Mariana
Costa, Ana Rodrigues
Antunes, Célia Maria
author_sort Queiroga, Maria Cristina
collection PubMed
description Antimicrobial resistance is a serious problem for the control of infections and infectious diseases. Propolis is a substance produced by honeybees with antimicrobial and antibiofilm properties. To consider propolis as an alternative to the use of antimicrobials for infection control, we assessed its antimicrobial and antibiofilm activities. To assess propolis for topical medical use, toxicological studies were also performed. A Portuguese 70% propolis ethanolic extract was chemically evaluated and studied for antimicrobial activity on staphylococcal field isolates (n = 137) and antibiofilm action (n = 45). Cell toxicological assessment was performed using keratinocytes and fibroblasts. Pinobanksin, chrysin, acacetin, apigenin, pinocembrin, and kaempferol-dimethyl-ether were detected. All 137 isolates were susceptible to 6.68 mg/mL or lower propolis concentration (80% isolates were susceptible to <1 mg/mL). The mean percentage of biofilm inhibition was 71%, and biofilm disruption was 88.5%. Propolis (<1 mg/mL) was well-tolerated by fibroblasts and moderately tolerated by keratinocytes. The combined antimicrobial and antibiofilm effect of propolis, together with its low toxicity to connective tissue and epithelial cells, suggests a good applicability for topical antibacterial treatment. Therefore, propolis seems to be a good alternative to antimicrobials for the treatment of infections with Staphylococcus spp. that deserves to be evaluated in vivo.
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spelling pubmed-99520622023-02-25 Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis Queiroga, Maria Cristina Laranjo, Marta Andrade, Nara Marques, Mariana Costa, Ana Rodrigues Antunes, Célia Maria Antibiotics (Basel) Article Antimicrobial resistance is a serious problem for the control of infections and infectious diseases. Propolis is a substance produced by honeybees with antimicrobial and antibiofilm properties. To consider propolis as an alternative to the use of antimicrobials for infection control, we assessed its antimicrobial and antibiofilm activities. To assess propolis for topical medical use, toxicological studies were also performed. A Portuguese 70% propolis ethanolic extract was chemically evaluated and studied for antimicrobial activity on staphylococcal field isolates (n = 137) and antibiofilm action (n = 45). Cell toxicological assessment was performed using keratinocytes and fibroblasts. Pinobanksin, chrysin, acacetin, apigenin, pinocembrin, and kaempferol-dimethyl-ether were detected. All 137 isolates were susceptible to 6.68 mg/mL or lower propolis concentration (80% isolates were susceptible to <1 mg/mL). The mean percentage of biofilm inhibition was 71%, and biofilm disruption was 88.5%. Propolis (<1 mg/mL) was well-tolerated by fibroblasts and moderately tolerated by keratinocytes. The combined antimicrobial and antibiofilm effect of propolis, together with its low toxicity to connective tissue and epithelial cells, suggests a good applicability for topical antibacterial treatment. Therefore, propolis seems to be a good alternative to antimicrobials for the treatment of infections with Staphylococcus spp. that deserves to be evaluated in vivo. MDPI 2023-02-08 /pmc/articles/PMC9952062/ /pubmed/36830258 http://dx.doi.org/10.3390/antibiotics12020347 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Queiroga, Maria Cristina
Laranjo, Marta
Andrade, Nara
Marques, Mariana
Costa, Ana Rodrigues
Antunes, Célia Maria
Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis
title Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis
title_full Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis
title_fullStr Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis
title_full_unstemmed Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis
title_short Antimicrobial, Antibiofilm and Toxicological Assessment of Propolis
title_sort antimicrobial, antibiofilm and toxicological assessment of propolis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952062/
https://www.ncbi.nlm.nih.gov/pubmed/36830258
http://dx.doi.org/10.3390/antibiotics12020347
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