Hydrogen Peroxide and GA(3) Levels Regulate the High Night Temperature Response in Pistils of Wheat (Triticum aestivum L.)

High night temperature (HNT) impairs crop productivity through the reproductive failure of gametes (pollen and pistil). Though female gametophyte (pistil) is an equal partner in the seed-set, the knowledge of the antioxidant system(s) and hormonal control of HNT tolerance or susceptibility of pistils is limited and lacking. The objectives of this study were to determine the antioxidant mechanism for homeostatic control of free radicals, and the involvement of abscisic acid (ABA) and gibberellic acid (GA(3)) in HNT stress protection in the wheat pistils of contrasting wheat genotypes. We hypothesized that HNT tolerance is attributed to the homeostatic control of reactive oxygen species (ROS) and hormonal readjustment in pistils of the tolerant genotype. The ears of two contrasting wheat genotypes—HD 2329 (susceptible) and Raj 3765 (tolerant) were subjected to two HNTs (+5 °C and +8 °C) over ambient, in the absence and presence of dimethylthiourea (DMTU), a chemical trap of hydrogen peroxide (H(2)O(2)). Results showed that HNTs significantly increased ROS in pistils of susceptible genotype HD 2329 to a relatively greater extent compared to tolerant genotype Raj 3765. The response was similar in the presence or absence of DMTU, but the H(2)O(2) values were lower in the presence of DMTU. The ROS levels were balanced by increased activity of peroxidase under HNT to a greater extent in the tolerant genotype. Cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) activity was inversely related to H(2)O(2) production within a critical range in Raj 3765, indicating its modulation by H(2)O(2) levels as no change was observed at the transcriptional level. The hormonal status showed increased ABA and decreased GA(3) contents with increasing temperature. Our study elucidates the role of H(2)O(2) and GA(3) in stress tolerance of pistils of tolerant genotype where GAPC acts as a ROS sensor due to H(2)O(2)-mediated decrease in its activity.