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Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors

During the process of adapting to metal contamination, plants produce secondary metabolites that have the potential to modulate multidrug-resistant (MDR) phenotypes; this is achieved by inhibiting the activity of efflux pumps to reduce the minimum inhibitory concentrations (MICs) of antimicrobial su...

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Autores principales: Nguyen, Thi Huyen Thu, Nguyen, Ngoc Anh Thơ, Nguyen, Hai Dang, Nguyen, Thi Thu Hien, Le, Mai Huong, Pham, Minh Quan, Do, Huu Nghi, Hoang, Kim Chi, Michalet, Serge, Dijoux-Franca, Marie-Geneviève, Pham, Hoang Nam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952282/
https://www.ncbi.nlm.nih.gov/pubmed/36830331
http://dx.doi.org/10.3390/antibiotics12020421
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author Nguyen, Thi Huyen Thu
Nguyen, Ngoc Anh Thơ
Nguyen, Hai Dang
Nguyen, Thi Thu Hien
Le, Mai Huong
Pham, Minh Quan
Do, Huu Nghi
Hoang, Kim Chi
Michalet, Serge
Dijoux-Franca, Marie-Geneviève
Pham, Hoang Nam
author_facet Nguyen, Thi Huyen Thu
Nguyen, Ngoc Anh Thơ
Nguyen, Hai Dang
Nguyen, Thi Thu Hien
Le, Mai Huong
Pham, Minh Quan
Do, Huu Nghi
Hoang, Kim Chi
Michalet, Serge
Dijoux-Franca, Marie-Geneviève
Pham, Hoang Nam
author_sort Nguyen, Thi Huyen Thu
collection PubMed
description During the process of adapting to metal contamination, plants produce secondary metabolites that have the potential to modulate multidrug-resistant (MDR) phenotypes; this is achieved by inhibiting the activity of efflux pumps to reduce the minimum inhibitory concentrations (MICs) of antimicrobial substrates. Our study evaluated the effect of secondary metabolites of belowground parts of Pteris vittata L. and Fallopia japonica, two metal-tolerant plants from northern Vietnam, on six antibiotic-resistant Stenotrophomonas maltophilia strains possessing efflux pump resistance mechanisms that were isolated from soil and clinical samples. The chemical composition of aqueous and dichloromethane (DCM) fractions extracted from P. vittata and F. japonica was determined using UHPLC-DAD-ESI/QTOF analysis. The antibacterial and efflux pump inhibitory activities of the four fractions were evaluated for the six strains (K279a, 0366, BurA1, BurE1, PierC1, and 502) using a microdilution assay at fraction concentrations of 62.5, 125, and 250 μg/mL. The DCM fraction of F. japonica exhibited remarkable antibacterial activity against strain 0366, with a MIC of 31.25 μg/mL. Furthermore, this fraction also significantly decreased gentamicin MIC: four-fold and eight-fold reductions for BurA1 and BurE1 strains, respectively (when tested at 250 μg/mL), and two-fold and eight-fold reductions for K279a and BurE1 strains, respectively (when tested at 125 μg/mL). Pure emodin, the main component identified in the DCM fraction of F. japonica, and sennidine A&B only reduced by half the MIC of gentamicin (when tested at 30 μg/mL). Our results suggest that the DCM fraction components of F. japonica underground parts may be potential candidates for new bacterial efflux pump inhibitors (EPIs).
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spelling pubmed-99522822023-02-25 Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors Nguyen, Thi Huyen Thu Nguyen, Ngoc Anh Thơ Nguyen, Hai Dang Nguyen, Thi Thu Hien Le, Mai Huong Pham, Minh Quan Do, Huu Nghi Hoang, Kim Chi Michalet, Serge Dijoux-Franca, Marie-Geneviève Pham, Hoang Nam Antibiotics (Basel) Article During the process of adapting to metal contamination, plants produce secondary metabolites that have the potential to modulate multidrug-resistant (MDR) phenotypes; this is achieved by inhibiting the activity of efflux pumps to reduce the minimum inhibitory concentrations (MICs) of antimicrobial substrates. Our study evaluated the effect of secondary metabolites of belowground parts of Pteris vittata L. and Fallopia japonica, two metal-tolerant plants from northern Vietnam, on six antibiotic-resistant Stenotrophomonas maltophilia strains possessing efflux pump resistance mechanisms that were isolated from soil and clinical samples. The chemical composition of aqueous and dichloromethane (DCM) fractions extracted from P. vittata and F. japonica was determined using UHPLC-DAD-ESI/QTOF analysis. The antibacterial and efflux pump inhibitory activities of the four fractions were evaluated for the six strains (K279a, 0366, BurA1, BurE1, PierC1, and 502) using a microdilution assay at fraction concentrations of 62.5, 125, and 250 μg/mL. The DCM fraction of F. japonica exhibited remarkable antibacterial activity against strain 0366, with a MIC of 31.25 μg/mL. Furthermore, this fraction also significantly decreased gentamicin MIC: four-fold and eight-fold reductions for BurA1 and BurE1 strains, respectively (when tested at 250 μg/mL), and two-fold and eight-fold reductions for K279a and BurE1 strains, respectively (when tested at 125 μg/mL). Pure emodin, the main component identified in the DCM fraction of F. japonica, and sennidine A&B only reduced by half the MIC of gentamicin (when tested at 30 μg/mL). Our results suggest that the DCM fraction components of F. japonica underground parts may be potential candidates for new bacterial efflux pump inhibitors (EPIs). MDPI 2023-02-20 /pmc/articles/PMC9952282/ /pubmed/36830331 http://dx.doi.org/10.3390/antibiotics12020421 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nguyen, Thi Huyen Thu
Nguyen, Ngoc Anh Thơ
Nguyen, Hai Dang
Nguyen, Thi Thu Hien
Le, Mai Huong
Pham, Minh Quan
Do, Huu Nghi
Hoang, Kim Chi
Michalet, Serge
Dijoux-Franca, Marie-Geneviève
Pham, Hoang Nam
Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors
title Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors
title_full Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors
title_fullStr Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors
title_full_unstemmed Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors
title_short Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant Stenotrophomonas Maltophilia: Potential of Herbal-Derived Efflux Pump Inhibitors
title_sort plant secondary metabolites on efflux-mediated antibiotic resistant stenotrophomonas maltophilia: potential of herbal-derived efflux pump inhibitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952282/
https://www.ncbi.nlm.nih.gov/pubmed/36830331
http://dx.doi.org/10.3390/antibiotics12020421
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