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Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)

Chloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did...

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Autores principales: Jardim-Messeder, Douglas, Caverzan, Andreia, Balbinott, Natalia, Menguer, Paloma K., Paiva, Ana L. S., Lemos, Moaciria, Cunha, Juliana R., Gaeta, Marcos L., Costa, Miguel, Zamocky, Marcel, Saibo, Nelson J. M., Silveira, Joaquim A. G., Margis, Rogério, Margis-Pinheiro, Márcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952370/
https://www.ncbi.nlm.nih.gov/pubmed/36829946
http://dx.doi.org/10.3390/antiox12020387
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author Jardim-Messeder, Douglas
Caverzan, Andreia
Balbinott, Natalia
Menguer, Paloma K.
Paiva, Ana L. S.
Lemos, Moaciria
Cunha, Juliana R.
Gaeta, Marcos L.
Costa, Miguel
Zamocky, Marcel
Saibo, Nelson J. M.
Silveira, Joaquim A. G.
Margis, Rogério
Margis-Pinheiro, Márcia
author_facet Jardim-Messeder, Douglas
Caverzan, Andreia
Balbinott, Natalia
Menguer, Paloma K.
Paiva, Ana L. S.
Lemos, Moaciria
Cunha, Juliana R.
Gaeta, Marcos L.
Costa, Miguel
Zamocky, Marcel
Saibo, Nelson J. M.
Silveira, Joaquim A. G.
Margis, Rogério
Margis-Pinheiro, Márcia
author_sort Jardim-Messeder, Douglas
collection PubMed
description Chloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did not impact plant growth, redox state, or photosynthesis parameters. Nevertheless, when subjected to drought stress, silenced plants (APX7i) show a higher capacity to maintain stomata aperture and photosynthesis performance, resulting in a higher tolerance when compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 did not lead to changes in the global expression of genes related to reactive oxygen species metabolism. In addition, the drought-mediated induction of several genes related to the proteasome pathway and the down-regulation of genes related to nitrogen and carotenoid metabolism was impaired in APX7i plants. During drought stress, APX7i showed an up-regulation of genes encoding flavonoid and tyrosine metabolism enzymes and a down-regulation of genes related to phytohormones signal transduction and nicotinate and nicotinamide metabolism. Our results demonstrate that OsAPX7 might be involved in signaling transduction pathways related to drought stress response, contributing to the understanding of the physiological role of chloroplast APX isoforms in rice.
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spelling pubmed-99523702023-02-25 Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa) Jardim-Messeder, Douglas Caverzan, Andreia Balbinott, Natalia Menguer, Paloma K. Paiva, Ana L. S. Lemos, Moaciria Cunha, Juliana R. Gaeta, Marcos L. Costa, Miguel Zamocky, Marcel Saibo, Nelson J. M. Silveira, Joaquim A. G. Margis, Rogério Margis-Pinheiro, Márcia Antioxidants (Basel) Article Chloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did not impact plant growth, redox state, or photosynthesis parameters. Nevertheless, when subjected to drought stress, silenced plants (APX7i) show a higher capacity to maintain stomata aperture and photosynthesis performance, resulting in a higher tolerance when compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 did not lead to changes in the global expression of genes related to reactive oxygen species metabolism. In addition, the drought-mediated induction of several genes related to the proteasome pathway and the down-regulation of genes related to nitrogen and carotenoid metabolism was impaired in APX7i plants. During drought stress, APX7i showed an up-regulation of genes encoding flavonoid and tyrosine metabolism enzymes and a down-regulation of genes related to phytohormones signal transduction and nicotinate and nicotinamide metabolism. Our results demonstrate that OsAPX7 might be involved in signaling transduction pathways related to drought stress response, contributing to the understanding of the physiological role of chloroplast APX isoforms in rice. MDPI 2023-02-05 /pmc/articles/PMC9952370/ /pubmed/36829946 http://dx.doi.org/10.3390/antiox12020387 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jardim-Messeder, Douglas
Caverzan, Andreia
Balbinott, Natalia
Menguer, Paloma K.
Paiva, Ana L. S.
Lemos, Moaciria
Cunha, Juliana R.
Gaeta, Marcos L.
Costa, Miguel
Zamocky, Marcel
Saibo, Nelson J. M.
Silveira, Joaquim A. G.
Margis, Rogério
Margis-Pinheiro, Márcia
Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_full Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_fullStr Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_full_unstemmed Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_short Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_sort stromal ascorbate peroxidase (osapx7) modulates drought stress tolerance in rice (oryza sativa)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952370/
https://www.ncbi.nlm.nih.gov/pubmed/36829946
http://dx.doi.org/10.3390/antiox12020387
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