Cargando…
Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism
Ginsenosides, active substances in Panax ginseng C. A. Meyer (ginseng), extend lifespan in multiple species, ameliorate age-associated damage, and limit functional decline in multiple tissues. However, their active components and their molecular mechanisms are largely unknown. Here, ginsenoside Rg1...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952469/ https://www.ncbi.nlm.nih.gov/pubmed/36829855 http://dx.doi.org/10.3390/antiox12020296 |
_version_ | 1784893639229440000 |
---|---|
author | Wang, Siming Qiao, Juhui Jiang, Chunyan Pan, Daian Yu, Shiting Chen, Jingjing Liu, Shichao Zhang, Peiguang Zhao, Daqing Liu, Meichen |
author_facet | Wang, Siming Qiao, Juhui Jiang, Chunyan Pan, Daian Yu, Shiting Chen, Jingjing Liu, Shichao Zhang, Peiguang Zhao, Daqing Liu, Meichen |
author_sort | Wang, Siming |
collection | PubMed |
description | Ginsenosides, active substances in Panax ginseng C. A. Meyer (ginseng), extend lifespan in multiple species, ameliorate age-associated damage, and limit functional decline in multiple tissues. However, their active components and their molecular mechanisms are largely unknown. Here, ginsenoside Rg1 (Rg1) promoted longevity in Saccharomyces cerevisiae. Treatment with Rg1 decreased aging-mediated surface wrinkling, enhanced stress resistance, decreased reactive oxygen species’ production and apoptosis, improved antioxidant enzyme activity, and decreased the aging rate. Proteomic analysis indicated that Rg1 delays S. cerevisiae senescence by regulating metabolic homeostasis. Protein–protein interaction networks based on differential protein expression indicated that CDC19, a homologue of pyruvate kinase, and SDH2, the succinate dehydrogenase iron–sulfur protein subunit, might be the effector proteins involved in the regulation by Rg1. Further experiments confirmed that Rg1 improved specific parameters of mitochondrial bioenergetics and core enzymes in the glycolytic pathway. Mutant strains were constructed that demonstrated the relationships between metabolic homeostasis and the predicted target proteins of Rg1. Rg1 could be used in new treatments for slowing the aging process. Our results also provide a useful dataset for further investigations of the mechanisms of ginseng in aging. |
format | Online Article Text |
id | pubmed-9952469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99524692023-02-25 Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism Wang, Siming Qiao, Juhui Jiang, Chunyan Pan, Daian Yu, Shiting Chen, Jingjing Liu, Shichao Zhang, Peiguang Zhao, Daqing Liu, Meichen Antioxidants (Basel) Article Ginsenosides, active substances in Panax ginseng C. A. Meyer (ginseng), extend lifespan in multiple species, ameliorate age-associated damage, and limit functional decline in multiple tissues. However, their active components and their molecular mechanisms are largely unknown. Here, ginsenoside Rg1 (Rg1) promoted longevity in Saccharomyces cerevisiae. Treatment with Rg1 decreased aging-mediated surface wrinkling, enhanced stress resistance, decreased reactive oxygen species’ production and apoptosis, improved antioxidant enzyme activity, and decreased the aging rate. Proteomic analysis indicated that Rg1 delays S. cerevisiae senescence by regulating metabolic homeostasis. Protein–protein interaction networks based on differential protein expression indicated that CDC19, a homologue of pyruvate kinase, and SDH2, the succinate dehydrogenase iron–sulfur protein subunit, might be the effector proteins involved in the regulation by Rg1. Further experiments confirmed that Rg1 improved specific parameters of mitochondrial bioenergetics and core enzymes in the glycolytic pathway. Mutant strains were constructed that demonstrated the relationships between metabolic homeostasis and the predicted target proteins of Rg1. Rg1 could be used in new treatments for slowing the aging process. Our results also provide a useful dataset for further investigations of the mechanisms of ginseng in aging. MDPI 2023-01-28 /pmc/articles/PMC9952469/ /pubmed/36829855 http://dx.doi.org/10.3390/antiox12020296 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Siming Qiao, Juhui Jiang, Chunyan Pan, Daian Yu, Shiting Chen, Jingjing Liu, Shichao Zhang, Peiguang Zhao, Daqing Liu, Meichen Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism |
title | Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism |
title_full | Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism |
title_fullStr | Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism |
title_full_unstemmed | Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism |
title_short | Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism |
title_sort | ginsenoside rg1 delays chronological aging in a yeast model via cdc19- and sdh2-mediated cellular metabolism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952469/ https://www.ncbi.nlm.nih.gov/pubmed/36829855 http://dx.doi.org/10.3390/antiox12020296 |
work_keys_str_mv | AT wangsiming ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT qiaojuhui ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT jiangchunyan ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT pandaian ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT yushiting ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT chenjingjing ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT liushichao ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT zhangpeiguang ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT zhaodaqing ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism AT liumeichen ginsenosiderg1delayschronologicalaginginayeastmodelviacdc19andsdh2mediatedcellularmetabolism |