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Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism

Ginsenosides, active substances in Panax ginseng C. A. Meyer (ginseng), extend lifespan in multiple species, ameliorate age-associated damage, and limit functional decline in multiple tissues. However, their active components and their molecular mechanisms are largely unknown. Here, ginsenoside Rg1...

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Autores principales: Wang, Siming, Qiao, Juhui, Jiang, Chunyan, Pan, Daian, Yu, Shiting, Chen, Jingjing, Liu, Shichao, Zhang, Peiguang, Zhao, Daqing, Liu, Meichen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952469/
https://www.ncbi.nlm.nih.gov/pubmed/36829855
http://dx.doi.org/10.3390/antiox12020296
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author Wang, Siming
Qiao, Juhui
Jiang, Chunyan
Pan, Daian
Yu, Shiting
Chen, Jingjing
Liu, Shichao
Zhang, Peiguang
Zhao, Daqing
Liu, Meichen
author_facet Wang, Siming
Qiao, Juhui
Jiang, Chunyan
Pan, Daian
Yu, Shiting
Chen, Jingjing
Liu, Shichao
Zhang, Peiguang
Zhao, Daqing
Liu, Meichen
author_sort Wang, Siming
collection PubMed
description Ginsenosides, active substances in Panax ginseng C. A. Meyer (ginseng), extend lifespan in multiple species, ameliorate age-associated damage, and limit functional decline in multiple tissues. However, their active components and their molecular mechanisms are largely unknown. Here, ginsenoside Rg1 (Rg1) promoted longevity in Saccharomyces cerevisiae. Treatment with Rg1 decreased aging-mediated surface wrinkling, enhanced stress resistance, decreased reactive oxygen species’ production and apoptosis, improved antioxidant enzyme activity, and decreased the aging rate. Proteomic analysis indicated that Rg1 delays S. cerevisiae senescence by regulating metabolic homeostasis. Protein–protein interaction networks based on differential protein expression indicated that CDC19, a homologue of pyruvate kinase, and SDH2, the succinate dehydrogenase iron–sulfur protein subunit, might be the effector proteins involved in the regulation by Rg1. Further experiments confirmed that Rg1 improved specific parameters of mitochondrial bioenergetics and core enzymes in the glycolytic pathway. Mutant strains were constructed that demonstrated the relationships between metabolic homeostasis and the predicted target proteins of Rg1. Rg1 could be used in new treatments for slowing the aging process. Our results also provide a useful dataset for further investigations of the mechanisms of ginseng in aging.
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spelling pubmed-99524692023-02-25 Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism Wang, Siming Qiao, Juhui Jiang, Chunyan Pan, Daian Yu, Shiting Chen, Jingjing Liu, Shichao Zhang, Peiguang Zhao, Daqing Liu, Meichen Antioxidants (Basel) Article Ginsenosides, active substances in Panax ginseng C. A. Meyer (ginseng), extend lifespan in multiple species, ameliorate age-associated damage, and limit functional decline in multiple tissues. However, their active components and their molecular mechanisms are largely unknown. Here, ginsenoside Rg1 (Rg1) promoted longevity in Saccharomyces cerevisiae. Treatment with Rg1 decreased aging-mediated surface wrinkling, enhanced stress resistance, decreased reactive oxygen species’ production and apoptosis, improved antioxidant enzyme activity, and decreased the aging rate. Proteomic analysis indicated that Rg1 delays S. cerevisiae senescence by regulating metabolic homeostasis. Protein–protein interaction networks based on differential protein expression indicated that CDC19, a homologue of pyruvate kinase, and SDH2, the succinate dehydrogenase iron–sulfur protein subunit, might be the effector proteins involved in the regulation by Rg1. Further experiments confirmed that Rg1 improved specific parameters of mitochondrial bioenergetics and core enzymes in the glycolytic pathway. Mutant strains were constructed that demonstrated the relationships between metabolic homeostasis and the predicted target proteins of Rg1. Rg1 could be used in new treatments for slowing the aging process. Our results also provide a useful dataset for further investigations of the mechanisms of ginseng in aging. MDPI 2023-01-28 /pmc/articles/PMC9952469/ /pubmed/36829855 http://dx.doi.org/10.3390/antiox12020296 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Siming
Qiao, Juhui
Jiang, Chunyan
Pan, Daian
Yu, Shiting
Chen, Jingjing
Liu, Shichao
Zhang, Peiguang
Zhao, Daqing
Liu, Meichen
Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism
title Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism
title_full Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism
title_fullStr Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism
title_full_unstemmed Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism
title_short Ginsenoside Rg1 Delays Chronological Aging in a Yeast Model via CDC19- and SDH2-Mediated Cellular Metabolism
title_sort ginsenoside rg1 delays chronological aging in a yeast model via cdc19- and sdh2-mediated cellular metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952469/
https://www.ncbi.nlm.nih.gov/pubmed/36829855
http://dx.doi.org/10.3390/antiox12020296
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