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Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice

With a gradually increasing elderly population, the treatment of geriatric patients represents a major challenge for trauma and reconstructive surgery. Although, it is well established that aging affects bone metabolism, it is still controversial if aging impairs bone healing. Accordingly, we invest...

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Autores principales: Menger, Maximilian M., Manuschewski, Ruben, Ehnert, Sabrina, Rollmann, Mika F., Maisenbacher, Tanja C., Tobias, Anne L., Menger, Michael D., Laschke, Matthias W., Histing, Tina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952563/
https://www.ncbi.nlm.nih.gov/pubmed/36829769
http://dx.doi.org/10.3390/bioengineering10020275
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author Menger, Maximilian M.
Manuschewski, Ruben
Ehnert, Sabrina
Rollmann, Mika F.
Maisenbacher, Tanja C.
Tobias, Anne L.
Menger, Michael D.
Laschke, Matthias W.
Histing, Tina
author_facet Menger, Maximilian M.
Manuschewski, Ruben
Ehnert, Sabrina
Rollmann, Mika F.
Maisenbacher, Tanja C.
Tobias, Anne L.
Menger, Michael D.
Laschke, Matthias W.
Histing, Tina
author_sort Menger, Maximilian M.
collection PubMed
description With a gradually increasing elderly population, the treatment of geriatric patients represents a major challenge for trauma and reconstructive surgery. Although, it is well established that aging affects bone metabolism, it is still controversial if aging impairs bone healing. Accordingly, we investigated fracture healing in young adult (3–4 months) and aged (16–18 months) CD-1 mice using a stable closed femoral fracture model. Bone healing was analyzed by radiographic, biomechanical and histological analysis at 1, 2, 3, 4 and 5 weeks after fracture. Our results demonstrated an increased callus diameter to femoral diameter ratio in aged animals at later time points of fracture healing when compared to young adult mice. Moreover, our biomechanical analysis revealed a significantly decreased bending stiffness at 3 and 4 weeks after fracture in aged animals. In contrast, at 5 weeks after fracture, the analysis showed no significant difference in bending stiffness between the two study groups. Additional histological analysis showed a delayed endochondral ossification in aged animals as well as a higher amounts of fibrous tissue at early healing time points. These findings indicate a delayed process of callus remodeling in aged CD-1 mice, resulting in a delayed fracture healing when compared to young adult animals. However, the overall healing capacity of the fractured femora was not affected by aging.
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spelling pubmed-99525632023-02-25 Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice Menger, Maximilian M. Manuschewski, Ruben Ehnert, Sabrina Rollmann, Mika F. Maisenbacher, Tanja C. Tobias, Anne L. Menger, Michael D. Laschke, Matthias W. Histing, Tina Bioengineering (Basel) Article With a gradually increasing elderly population, the treatment of geriatric patients represents a major challenge for trauma and reconstructive surgery. Although, it is well established that aging affects bone metabolism, it is still controversial if aging impairs bone healing. Accordingly, we investigated fracture healing in young adult (3–4 months) and aged (16–18 months) CD-1 mice using a stable closed femoral fracture model. Bone healing was analyzed by radiographic, biomechanical and histological analysis at 1, 2, 3, 4 and 5 weeks after fracture. Our results demonstrated an increased callus diameter to femoral diameter ratio in aged animals at later time points of fracture healing when compared to young adult mice. Moreover, our biomechanical analysis revealed a significantly decreased bending stiffness at 3 and 4 weeks after fracture in aged animals. In contrast, at 5 weeks after fracture, the analysis showed no significant difference in bending stiffness between the two study groups. Additional histological analysis showed a delayed endochondral ossification in aged animals as well as a higher amounts of fibrous tissue at early healing time points. These findings indicate a delayed process of callus remodeling in aged CD-1 mice, resulting in a delayed fracture healing when compared to young adult animals. However, the overall healing capacity of the fractured femora was not affected by aging. MDPI 2023-02-20 /pmc/articles/PMC9952563/ /pubmed/36829769 http://dx.doi.org/10.3390/bioengineering10020275 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Menger, Maximilian M.
Manuschewski, Ruben
Ehnert, Sabrina
Rollmann, Mika F.
Maisenbacher, Tanja C.
Tobias, Anne L.
Menger, Michael D.
Laschke, Matthias W.
Histing, Tina
Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice
title Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice
title_full Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice
title_fullStr Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice
title_full_unstemmed Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice
title_short Radiographic, Biomechanical and Histological Characterization of Femoral Fracture Healing in Aged CD-1 Mice
title_sort radiographic, biomechanical and histological characterization of femoral fracture healing in aged cd-1 mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952563/
https://www.ncbi.nlm.nih.gov/pubmed/36829769
http://dx.doi.org/10.3390/bioengineering10020275
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