Intra-Population Alteration on Voltinism of Asian Corn Borer in Response to Climate Warming

SIMPLE SUMMARY: The Asian corn borer (ACB), Ostrinia furnacalis, is a devastating corn pest widely distributed in China. Depending on the climate, ACB may have one or up to seven generations each year, from north to south, respectively. Previous investigations suggested co-existence of the uni- and multivoltine patterns in northeast regions (45°38′ N). Efforts have been conducted to isolate uni-voltinism (with obligate diapause), multi-voltinism (with facultative diapause), and a non-diapausing strain using recurrent selections under various simulated temperature and photoperiod environments. The univoltine (Lu) strain has evolved a stable univoltinism under a diapause suppressing condition (16 h daylength at 28 °C). The multivoltine strain (Lm) was shown to have a typical facultative diapause induced by a range of short-day lengths (11–13.5 h). The majority (94.4%) of the developed Ln strain still maintained the non-diapausing nature under a diapause enhancing condition, i.e., a short (13 h) daylength at a low temperature (22 °C). The study suggests that ACB has evolutionary intra-population variation in voltinism. Under the climate change scenario warmer spring and summer will affect the proportion of sympatric voltine biotype populations that evolve toward being multivoltine. ABSTRACT: The Asian corn borer (ACB) Ostrinia furnacalis (Guenée) can occur in one to seven generations annually from cool (48°00′ N) to warm (18°10′ N) region of corn cultivation in China. Although ACB is commonly known as a facultative larval diapause insect, the co-existence of various voltinism suggests that intra-population variation may have evolved for the nature of diapause, i.e., voltinism plasticity. Here, we conducted recurrent selection efforts to establish three strains of, respectively, univoltine (with obligate diapause), multivoltine (with facultative diapause), and non-diapausing ACB under various temperature and photoperiod environments. The univoltine (Lu) strain has evolved a stable univoltinism under a diapause suppressing condition (16 h daylength at 28 °C), with the diapause incidence constantly over 80% after three generations of selection. The multivoltine strain (Lm) under the high temperature (28 °C) was shown to have a typical facultative diapause induced by a range of short-day lengths (11–13.5 h). Diapause incidence was constantly <2.6% under the long day length (16 h) when the temperature was from 18 to 28 °C, i.e., low temperature could not enhance the diapause response in the Lm strain. However, the development was prolonged from 14.2 ± 0.3 d to 46.0 ± 0.8 d when the temperature was reduced from 28 °C to 18 °C. The majority (94.4%) of the developed Ln strain still maintained the non-diapausing nature under a diapause enhancing condition, i.e., a short (13 h) daylength at a low temperature (22 °C). Lm and Ln were able to complete their second generation in Heihe (50°14′ N) if the first-generation moth oviposits before 18 June. The study suggests that ACB has evolutionary intra-population variation in voltinism. Under the climate change scenario warmer spring and summer might affect the proportion of sympatric voltine biotype populations that evolve toward being multivoltine.