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Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation
This study presents a multilayer in vitro human skin platform to quantitatively relate predicted spatial time–temperature history with measured tissue injury response. This information is needed to elucidate high-temperature, short-duration burn injury kinetics and enables determination of relevant...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952576/ https://www.ncbi.nlm.nih.gov/pubmed/36829759 http://dx.doi.org/10.3390/bioengineering10020265 |
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author | Brocklehurst, Sean Ghousifam, Neda Zuniga, Kameel Stolley, Danielle Rylander, Marissa Nichole |
author_facet | Brocklehurst, Sean Ghousifam, Neda Zuniga, Kameel Stolley, Danielle Rylander, Marissa Nichole |
author_sort | Brocklehurst, Sean |
collection | PubMed |
description | This study presents a multilayer in vitro human skin platform to quantitatively relate predicted spatial time–temperature history with measured tissue injury response. This information is needed to elucidate high-temperature, short-duration burn injury kinetics and enables determination of relevant input parameters for computational models to facilitate treatment planning. Multilayer in vitro skin platforms were constructed using human dermal keratinocytes and fibroblasts embedded in collagen I hydrogels. After three seconds of contact with a 50–100 °C burn tip, ablation, cell death, apoptosis, and HSP70 expression were spatially measured using immunofluorescence confocal microscopy. Finite element modeling was performed using the measured thermal characteristics of skin platforms to determine the temperature distribution within platforms over time. The process coefficients for the Arrhenius thermal injury model describing tissue ablation and cell death were determined such that the predictions calculated from the time–temperature histories fit the experimental burn results. The activation energy for thermal collagen ablation and cell death was found to be significantly lower for short-duration, high-temperature burns than those found for long-duration, low-temperature burns. Analysis of results suggests that different injury mechanisms dominate at higher temperatures, necessitating burn research in the temperature ranges of interest and demonstrating the practicality of the proposed skin platform for this purpose. |
format | Online Article Text |
id | pubmed-9952576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99525762023-02-25 Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation Brocklehurst, Sean Ghousifam, Neda Zuniga, Kameel Stolley, Danielle Rylander, Marissa Nichole Bioengineering (Basel) Article This study presents a multilayer in vitro human skin platform to quantitatively relate predicted spatial time–temperature history with measured tissue injury response. This information is needed to elucidate high-temperature, short-duration burn injury kinetics and enables determination of relevant input parameters for computational models to facilitate treatment planning. Multilayer in vitro skin platforms were constructed using human dermal keratinocytes and fibroblasts embedded in collagen I hydrogels. After three seconds of contact with a 50–100 °C burn tip, ablation, cell death, apoptosis, and HSP70 expression were spatially measured using immunofluorescence confocal microscopy. Finite element modeling was performed using the measured thermal characteristics of skin platforms to determine the temperature distribution within platforms over time. The process coefficients for the Arrhenius thermal injury model describing tissue ablation and cell death were determined such that the predictions calculated from the time–temperature histories fit the experimental burn results. The activation energy for thermal collagen ablation and cell death was found to be significantly lower for short-duration, high-temperature burns than those found for long-duration, low-temperature burns. Analysis of results suggests that different injury mechanisms dominate at higher temperatures, necessitating burn research in the temperature ranges of interest and demonstrating the practicality of the proposed skin platform for this purpose. MDPI 2023-02-17 /pmc/articles/PMC9952576/ /pubmed/36829759 http://dx.doi.org/10.3390/bioengineering10020265 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brocklehurst, Sean Ghousifam, Neda Zuniga, Kameel Stolley, Danielle Rylander, Marissa Nichole Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation |
title | Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation |
title_full | Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation |
title_fullStr | Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation |
title_full_unstemmed | Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation |
title_short | Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury Investigation |
title_sort | multilayer in vitro human skin tissue platforms for quantitative burn injury investigation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952576/ https://www.ncbi.nlm.nih.gov/pubmed/36829759 http://dx.doi.org/10.3390/bioengineering10020265 |
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