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Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling

Human Fascia Lata (FL) is a connective tissue with a multilayered organization also known as aponeurotic fascia. FL biomechanics is influenced by its composite structure formed by fibrous layers (usually two) separated by loose connective tissue. In each layer, most of the collagen fibers run parall...

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Autores principales: Bonaldi, Lorenza, Berardo, Alice, Pirri, Carmelo, Stecco, Carla, Carniel, Emanuele Luigi, Fontanella, Chiara Giulia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952725/
https://www.ncbi.nlm.nih.gov/pubmed/36829719
http://dx.doi.org/10.3390/bioengineering10020226
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author Bonaldi, Lorenza
Berardo, Alice
Pirri, Carmelo
Stecco, Carla
Carniel, Emanuele Luigi
Fontanella, Chiara Giulia
author_facet Bonaldi, Lorenza
Berardo, Alice
Pirri, Carmelo
Stecco, Carla
Carniel, Emanuele Luigi
Fontanella, Chiara Giulia
author_sort Bonaldi, Lorenza
collection PubMed
description Human Fascia Lata (FL) is a connective tissue with a multilayered organization also known as aponeurotic fascia. FL biomechanics is influenced by its composite structure formed by fibrous layers (usually two) separated by loose connective tissue. In each layer, most of the collagen fibers run parallel in a distinct direction (with an interlayer angle that usually ranges from 75–80°), mirroring the fascia’s ability to adapt and withstand specific tensile loads. Although FL is a key structure in several musculoskeletal dysfunctions and in tissue engineering, literature still lacks the evidence that proves tissue anisotropy according to predominant collagen fiber directions. For this purpose, this work aims to analyze the biomechanical properties of ex-vivo FL (collected from fresh-frozen human donors) by performing uniaxial tensile tests in order to highlight any differences with respect to loading directions. The experimental outcomes showed a strong anisotropic behavior in accordance with principal collagen fibers directions, which characterize the composite structure. These findings have been implemented to propose a first constitutive model able to mimic the intra- and interlayer interactions. Both approaches could potentially support surgeons in daily practices (such as graft preparation and placement), engineers during in silico simulation, and physiotherapists during musculoskeletal rehabilitation, to customize a medical intervention based on each specific patient and clinical condition.
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spelling pubmed-99527252023-02-25 Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling Bonaldi, Lorenza Berardo, Alice Pirri, Carmelo Stecco, Carla Carniel, Emanuele Luigi Fontanella, Chiara Giulia Bioengineering (Basel) Article Human Fascia Lata (FL) is a connective tissue with a multilayered organization also known as aponeurotic fascia. FL biomechanics is influenced by its composite structure formed by fibrous layers (usually two) separated by loose connective tissue. In each layer, most of the collagen fibers run parallel in a distinct direction (with an interlayer angle that usually ranges from 75–80°), mirroring the fascia’s ability to adapt and withstand specific tensile loads. Although FL is a key structure in several musculoskeletal dysfunctions and in tissue engineering, literature still lacks the evidence that proves tissue anisotropy according to predominant collagen fiber directions. For this purpose, this work aims to analyze the biomechanical properties of ex-vivo FL (collected from fresh-frozen human donors) by performing uniaxial tensile tests in order to highlight any differences with respect to loading directions. The experimental outcomes showed a strong anisotropic behavior in accordance with principal collagen fibers directions, which characterize the composite structure. These findings have been implemented to propose a first constitutive model able to mimic the intra- and interlayer interactions. Both approaches could potentially support surgeons in daily practices (such as graft preparation and placement), engineers during in silico simulation, and physiotherapists during musculoskeletal rehabilitation, to customize a medical intervention based on each specific patient and clinical condition. MDPI 2023-02-07 /pmc/articles/PMC9952725/ /pubmed/36829719 http://dx.doi.org/10.3390/bioengineering10020226 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bonaldi, Lorenza
Berardo, Alice
Pirri, Carmelo
Stecco, Carla
Carniel, Emanuele Luigi
Fontanella, Chiara Giulia
Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling
title Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling
title_full Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling
title_fullStr Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling
title_full_unstemmed Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling
title_short Mechanical Characterization of Human Fascia Lata: Uniaxial Tensile Tests from Fresh-Frozen Cadaver Samples and Constitutive Modelling
title_sort mechanical characterization of human fascia lata: uniaxial tensile tests from fresh-frozen cadaver samples and constitutive modelling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952725/
https://www.ncbi.nlm.nih.gov/pubmed/36829719
http://dx.doi.org/10.3390/bioengineering10020226
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