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In Vivo Protein–Protein Binding Competition Assay Based on Split-GFP Reassembly: Proof of Concept
The split-green fluorescent protein (GFP) reassembly assay is a well-established approach to study protein–protein interactions (PPIs). In this assay, when two interacting proteins X and Y, respectively fused to residues 1–157 and to residues 158–237 of GFP, are co-expressed in E. coli, the two GFP...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9952896/ https://www.ncbi.nlm.nih.gov/pubmed/36830723 http://dx.doi.org/10.3390/biom13020354 |