Real-Time Ellipsometric Surface Plasmon Resonance Sensor Using Polarization Camera May Provide the Ultimate Detection Limit

Ellipsometric Surface Plasmon Resonance (SPR) sensors are known for their relatively simple optical configuration compared to interferometric and optical heterodyne phase interrogation techniques. However, most of the previously explored ellipsometric SPR sensors based on intensity measurements are limited by their real-time applications because phase or polarization shifts are conducted serially. Here we present an ellipsometric SPR sensor based on a Kretschmann–Raether (KR) diverging beam configuration and a pixelated microgrid polarization camera. The proposed methodology has the advantage of real-time and higher precision sensing applications. The short-term stability of the measurement using the ellipsometric parameters [Formula: see text] and [Formula: see text] is found to be superior over direct SPR or intensity measurements, particularly with fluctuating sources such as laser diodes. Refractive index and dynamic change measurements in real-time are presented together with Bovine Serum Albumin (BSA)–anti-BSA antibody binding to demonstrate the potential of the developed sensor for biological sensing applications with a resolution of sub-nM and down to pM with additional optimization. The analysis shows that this approach may provide the ultimate detection limit for SPR sensors.