Cargando…
Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome
Introduction: Polycystic ovarian syndrome (PCOS) is a common endocrine disorder amongst reproductive-age women, and 61% to 76% of women with PCOS are obese. Obese women with PCOS are usually burdened with infertility problems due to implantation failure. Thus, progesterone treatment is usually used...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953163/ https://www.ncbi.nlm.nih.gov/pubmed/36830813 http://dx.doi.org/10.3390/biomedicines11020276 |
_version_ | 1784893810017304576 |
---|---|
author | Sutaji, Zulazmi Abu, Muhammad Azrai Sayutti, Nurainie Elias, Marjanu Hikmah Ahmad, Mohd Faizal Nur Azurah, Abdul Ghani Chew, Kah Teik Abdul Karim, Abdul Kadir Aziz, Nor Haslinda Abd Mokhtar, Mohd Helmy Zin, Reena Rahayu Md Hussein, Zeti Azura Mohamed |
author_facet | Sutaji, Zulazmi Abu, Muhammad Azrai Sayutti, Nurainie Elias, Marjanu Hikmah Ahmad, Mohd Faizal Nur Azurah, Abdul Ghani Chew, Kah Teik Abdul Karim, Abdul Kadir Aziz, Nor Haslinda Abd Mokhtar, Mohd Helmy Zin, Reena Rahayu Md Hussein, Zeti Azura Mohamed |
author_sort | Sutaji, Zulazmi |
collection | PubMed |
description | Introduction: Polycystic ovarian syndrome (PCOS) is a common endocrine disorder amongst reproductive-age women, and 61% to 76% of women with PCOS are obese. Obese women with PCOS are usually burdened with infertility problems due to implantation failure. Thus, progesterone treatment is usually used to improve implantation rates. Although Hb-EGF expression is actively involved in endometrial receptivity and implantation, the data on heparin-binding epidermal growth factor (Hb-EGF) expression following progesterone therapy in obese women with PCOS are still lacking. Objective: To investigate the changes in serum follicle-stimulating hormone (FSH), luteinising hormone (LH), dehydroepiandrosterone sulphate (DHEA), progesterone and oestradiol levels and Hb-EGF expression in obese women with PCOS during the implantation window following progesterone therapy. Method: A total of 40 participants aged 18–40 years old were recruited following the provision of written consent. The participants were divided into the obese PCOS, normal-weight PCOS, obese fertile and normal-weight fertile groups. First blood collection was done before ovulation. Then, daily oral micronised progesterone (Utrogestan 200 mg) was given to the PCOS group for 10 days. The treatment was followed by a second blood collection and endometrial tissue sampling by using a Pipelle de Cornier catheter. In the fertile group, ovulation was confirmed by using ultrasound, and a second blood sample was collected on days 7 to 9 postovulation. The serum levels of FSH, LH, DHEA, progesterone and oestradiol were measured in all participants. Wilcoxon signed-rank test was used to compare FSH, LH, DHEA, progesterone and oestradiol levels during pre- and postovulation. Mann–Whitney test was performed to compare FSH, LH, DHEA, progesterone and oestradiol levels between two groups: (1) the PCOS group and the fertile group, (2) the obese PCOS group and the non-obese PCOS group and (3) the obese group and the non-obese fertile group. Result: Serum FSH levels were lower in obese women in their follicular phase than in women with normal weight regardless of their PCOS status, whereas serum LH/FSH ratios and DHEA levels were higher in women with PCOS than in women without PCOS. However, endometrial Hb-EGF expression was lower in the obese PCOS group than in the normal-weight PCOS group. Conclusions: Different patterns of hormonal levels and Hb-EGF expression levels were seen between the studied groups. However, further in vitro and in vivo studies are needed to investigate the mechanism underlying the changes in FSH, LH/FSH ratio, DHEA and Hb-EGF expression in PCOS after progesterone treatment. |
format | Online Article Text |
id | pubmed-9953163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99531632023-02-25 Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome Sutaji, Zulazmi Abu, Muhammad Azrai Sayutti, Nurainie Elias, Marjanu Hikmah Ahmad, Mohd Faizal Nur Azurah, Abdul Ghani Chew, Kah Teik Abdul Karim, Abdul Kadir Aziz, Nor Haslinda Abd Mokhtar, Mohd Helmy Zin, Reena Rahayu Md Hussein, Zeti Azura Mohamed Biomedicines Article Introduction: Polycystic ovarian syndrome (PCOS) is a common endocrine disorder amongst reproductive-age women, and 61% to 76% of women with PCOS are obese. Obese women with PCOS are usually burdened with infertility problems due to implantation failure. Thus, progesterone treatment is usually used to improve implantation rates. Although Hb-EGF expression is actively involved in endometrial receptivity and implantation, the data on heparin-binding epidermal growth factor (Hb-EGF) expression following progesterone therapy in obese women with PCOS are still lacking. Objective: To investigate the changes in serum follicle-stimulating hormone (FSH), luteinising hormone (LH), dehydroepiandrosterone sulphate (DHEA), progesterone and oestradiol levels and Hb-EGF expression in obese women with PCOS during the implantation window following progesterone therapy. Method: A total of 40 participants aged 18–40 years old were recruited following the provision of written consent. The participants were divided into the obese PCOS, normal-weight PCOS, obese fertile and normal-weight fertile groups. First blood collection was done before ovulation. Then, daily oral micronised progesterone (Utrogestan 200 mg) was given to the PCOS group for 10 days. The treatment was followed by a second blood collection and endometrial tissue sampling by using a Pipelle de Cornier catheter. In the fertile group, ovulation was confirmed by using ultrasound, and a second blood sample was collected on days 7 to 9 postovulation. The serum levels of FSH, LH, DHEA, progesterone and oestradiol were measured in all participants. Wilcoxon signed-rank test was used to compare FSH, LH, DHEA, progesterone and oestradiol levels during pre- and postovulation. Mann–Whitney test was performed to compare FSH, LH, DHEA, progesterone and oestradiol levels between two groups: (1) the PCOS group and the fertile group, (2) the obese PCOS group and the non-obese PCOS group and (3) the obese group and the non-obese fertile group. Result: Serum FSH levels were lower in obese women in their follicular phase than in women with normal weight regardless of their PCOS status, whereas serum LH/FSH ratios and DHEA levels were higher in women with PCOS than in women without PCOS. However, endometrial Hb-EGF expression was lower in the obese PCOS group than in the normal-weight PCOS group. Conclusions: Different patterns of hormonal levels and Hb-EGF expression levels were seen between the studied groups. However, further in vitro and in vivo studies are needed to investigate the mechanism underlying the changes in FSH, LH/FSH ratio, DHEA and Hb-EGF expression in PCOS after progesterone treatment. MDPI 2023-01-19 /pmc/articles/PMC9953163/ /pubmed/36830813 http://dx.doi.org/10.3390/biomedicines11020276 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sutaji, Zulazmi Abu, Muhammad Azrai Sayutti, Nurainie Elias, Marjanu Hikmah Ahmad, Mohd Faizal Nur Azurah, Abdul Ghani Chew, Kah Teik Abdul Karim, Abdul Kadir Aziz, Nor Haslinda Abd Mokhtar, Mohd Helmy Zin, Reena Rahayu Md Hussein, Zeti Azura Mohamed Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome |
title | Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome |
title_full | Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome |
title_fullStr | Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome |
title_full_unstemmed | Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome |
title_short | Endometrial Heparin-Binding Epidermal Growth Factor Gene Expression and Hormone Level Changes in Implantation Window of Obese Women with Polycystic Ovarian Syndrome |
title_sort | endometrial heparin-binding epidermal growth factor gene expression and hormone level changes in implantation window of obese women with polycystic ovarian syndrome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953163/ https://www.ncbi.nlm.nih.gov/pubmed/36830813 http://dx.doi.org/10.3390/biomedicines11020276 |
work_keys_str_mv | AT sutajizulazmi endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT abumuhammadazrai endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT sayuttinurainie endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT eliasmarjanuhikmah endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT ahmadmohdfaizal endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT nurazurahabdulghani endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT chewkahteik endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT abdulkarimabdulkadir endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT aziznorhaslindaabd endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT mokhtarmohdhelmy endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT zinreenarahayumd endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome AT husseinzetiazuramohamed endometrialheparinbindingepidermalgrowthfactorgeneexpressionandhormonelevelchangesinimplantationwindowofobesewomenwithpolycysticovariansyndrome |