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Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound

The aim of the study was to investigate the dynamics of the state of allo- and autografts of skin on a wound using optical modalities: diffuse reflectance spectroscopy (DRS), optical coherence tomography (OCT), and laser Doppler flowmetry (LDF). A deep thermal burn was simulated in 24 rats covering...

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Autores principales: Turchin, Ilya, Beschastnov, Vladimir, Peretyagin, Petr, Perekatova, Valeriya, Kostyuk, Alexey, Orlova, Anna, Koloshein, Nikita, Khilov, Aleksandr, Sergeeva, Ekaterina, Kirillin, Mikhail, Ryabkov, Maksim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953239/
https://www.ncbi.nlm.nih.gov/pubmed/36830888
http://dx.doi.org/10.3390/biomedicines11020351
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author Turchin, Ilya
Beschastnov, Vladimir
Peretyagin, Petr
Perekatova, Valeriya
Kostyuk, Alexey
Orlova, Anna
Koloshein, Nikita
Khilov, Aleksandr
Sergeeva, Ekaterina
Kirillin, Mikhail
Ryabkov, Maksim
author_facet Turchin, Ilya
Beschastnov, Vladimir
Peretyagin, Petr
Perekatova, Valeriya
Kostyuk, Alexey
Orlova, Anna
Koloshein, Nikita
Khilov, Aleksandr
Sergeeva, Ekaterina
Kirillin, Mikhail
Ryabkov, Maksim
author_sort Turchin, Ilya
collection PubMed
description The aim of the study was to investigate the dynamics of the state of allo- and autografts of skin on a wound using optical modalities: diffuse reflectance spectroscopy (DRS), optical coherence tomography (OCT), and laser Doppler flowmetry (LDF). A deep thermal burn was simulated in 24 rats covering 20% of the body surface. On day 3 after the injury, a fascial necrectomy of two 500 mm(2) areas on the left and right sides of the midline of the animal body were excised. Allografts and autografts were placed in the centers of these areas. Optical measurements of grafts were performed on the 0, 3rd, 6th, 10th, and 13th days after transplantation. The allografts demonstrated a pronounced decrease in oxygenation, blood content, and perfusion compared to autografts on the 6th day; in the following days of observation, these values returned to the average values of autografts. Water content gradually decreased from the beginning to the end of observation. In conclusion, optical diagnostics revealed changes in the morphological microstructure, the rate of restoration of blood circulation, and oxygen exchange in the early stages, specific for the allo- and autograft.
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spelling pubmed-99532392023-02-25 Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound Turchin, Ilya Beschastnov, Vladimir Peretyagin, Petr Perekatova, Valeriya Kostyuk, Alexey Orlova, Anna Koloshein, Nikita Khilov, Aleksandr Sergeeva, Ekaterina Kirillin, Mikhail Ryabkov, Maksim Biomedicines Article The aim of the study was to investigate the dynamics of the state of allo- and autografts of skin on a wound using optical modalities: diffuse reflectance spectroscopy (DRS), optical coherence tomography (OCT), and laser Doppler flowmetry (LDF). A deep thermal burn was simulated in 24 rats covering 20% of the body surface. On day 3 after the injury, a fascial necrectomy of two 500 mm(2) areas on the left and right sides of the midline of the animal body were excised. Allografts and autografts were placed in the centers of these areas. Optical measurements of grafts were performed on the 0, 3rd, 6th, 10th, and 13th days after transplantation. The allografts demonstrated a pronounced decrease in oxygenation, blood content, and perfusion compared to autografts on the 6th day; in the following days of observation, these values returned to the average values of autografts. Water content gradually decreased from the beginning to the end of observation. In conclusion, optical diagnostics revealed changes in the morphological microstructure, the rate of restoration of blood circulation, and oxygen exchange in the early stages, specific for the allo- and autograft. MDPI 2023-01-26 /pmc/articles/PMC9953239/ /pubmed/36830888 http://dx.doi.org/10.3390/biomedicines11020351 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turchin, Ilya
Beschastnov, Vladimir
Peretyagin, Petr
Perekatova, Valeriya
Kostyuk, Alexey
Orlova, Anna
Koloshein, Nikita
Khilov, Aleksandr
Sergeeva, Ekaterina
Kirillin, Mikhail
Ryabkov, Maksim
Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound
title Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound
title_full Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound
title_fullStr Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound
title_full_unstemmed Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound
title_short Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound
title_sort multimodal optical monitoring of auto- and allografts of skin on a burn wound
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953239/
https://www.ncbi.nlm.nih.gov/pubmed/36830888
http://dx.doi.org/10.3390/biomedicines11020351
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