Cargando…

SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction

Ischemic heart disease (IHD) is one of the main focuses in today’s healthcare due to its implications and complications, and it is predicted to be increasing in prevalence due to the ageing population. Although the conventional pharmacological and interventional methods for the treatment of IHD pres...

Descripción completa

Detalles Bibliográficos
Autores principales: Bajdak-Rusinek, Karolina, Fus-Kujawa, Agnieszka, Buszman, Piotr, Żyła-Uklejewicz, Dorota, Jelonek, Katarzyna, Musiał-Kulik, Monika, Fernandez, Carlos, Michalak, Magdalena, George, Kurian, Kasperczyk, Janusz, Buszman, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953248/
https://www.ncbi.nlm.nih.gov/pubmed/36830880
http://dx.doi.org/10.3390/biomedicines11020343
_version_ 1784893831120945152
author Bajdak-Rusinek, Karolina
Fus-Kujawa, Agnieszka
Buszman, Piotr
Żyła-Uklejewicz, Dorota
Jelonek, Katarzyna
Musiał-Kulik, Monika
Fernandez, Carlos
Michalak, Magdalena
George, Kurian
Kasperczyk, Janusz
Buszman, Paweł
author_facet Bajdak-Rusinek, Karolina
Fus-Kujawa, Agnieszka
Buszman, Piotr
Żyła-Uklejewicz, Dorota
Jelonek, Katarzyna
Musiał-Kulik, Monika
Fernandez, Carlos
Michalak, Magdalena
George, Kurian
Kasperczyk, Janusz
Buszman, Paweł
author_sort Bajdak-Rusinek, Karolina
collection PubMed
description Ischemic heart disease (IHD) is one of the main focuses in today’s healthcare due to its implications and complications, and it is predicted to be increasing in prevalence due to the ageing population. Although the conventional pharmacological and interventional methods for the treatment of IHD presents with success in the clinical setting, the long-term complications of cardiac insufficiency are on a continual incline as a result of post-infarction remodeling of the cardiac tissue. The migration and involvement of stem cells to the cardiac muscle, followed by differentiation into cardiac myocytes, has been proven to be the natural process, though at a slow rate. SDF-1α is a novel candidate to mobilize stem cells homing to the ischemic heart. Endogenous SDF-1α levels are elevated after myocardial infarction, but their presence gradually decreases after approximately seven days. Additional administration of SDF-1α-releasing microspheres could be a tool for the extension of the time the stem cells are in the cardiac tissue after myocardial infarction. This, in turn, could constitute a novel therapy for more efficient regeneration of the heart muscle after injury. Through this practical study, it has been shown that the controlled release of SDF-1α from biodegradable microspheres into the pericardial sac fourteen days after myocardial infarction increases the concentration of exogenous SDF-1α, which persists in the tissue much longer than the level of endogenous SDF-1α. In addition, administration of SDF-1α-releasing microspheres increased the expression of the factors potentially involved in the involvement and retention of myocardial stem cells, which constitutes vascular endothelial growth factor A (VEGFA), stem cell factor (SCF), and vascular cell adhesion molecules (VCAMs) at the site of damaged tissue. This exhibits the possibility of combating the basic limitations of cell therapy, including ineffective stem cell implantation and the ability to induce the migration of endogenous stem cells to the ischemic cardiac tissue and promote heart repair.
format Online
Article
Text
id pubmed-9953248
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99532482023-02-25 SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction Bajdak-Rusinek, Karolina Fus-Kujawa, Agnieszka Buszman, Piotr Żyła-Uklejewicz, Dorota Jelonek, Katarzyna Musiał-Kulik, Monika Fernandez, Carlos Michalak, Magdalena George, Kurian Kasperczyk, Janusz Buszman, Paweł Biomedicines Article Ischemic heart disease (IHD) is one of the main focuses in today’s healthcare due to its implications and complications, and it is predicted to be increasing in prevalence due to the ageing population. Although the conventional pharmacological and interventional methods for the treatment of IHD presents with success in the clinical setting, the long-term complications of cardiac insufficiency are on a continual incline as a result of post-infarction remodeling of the cardiac tissue. The migration and involvement of stem cells to the cardiac muscle, followed by differentiation into cardiac myocytes, has been proven to be the natural process, though at a slow rate. SDF-1α is a novel candidate to mobilize stem cells homing to the ischemic heart. Endogenous SDF-1α levels are elevated after myocardial infarction, but their presence gradually decreases after approximately seven days. Additional administration of SDF-1α-releasing microspheres could be a tool for the extension of the time the stem cells are in the cardiac tissue after myocardial infarction. This, in turn, could constitute a novel therapy for more efficient regeneration of the heart muscle after injury. Through this practical study, it has been shown that the controlled release of SDF-1α from biodegradable microspheres into the pericardial sac fourteen days after myocardial infarction increases the concentration of exogenous SDF-1α, which persists in the tissue much longer than the level of endogenous SDF-1α. In addition, administration of SDF-1α-releasing microspheres increased the expression of the factors potentially involved in the involvement and retention of myocardial stem cells, which constitutes vascular endothelial growth factor A (VEGFA), stem cell factor (SCF), and vascular cell adhesion molecules (VCAMs) at the site of damaged tissue. This exhibits the possibility of combating the basic limitations of cell therapy, including ineffective stem cell implantation and the ability to induce the migration of endogenous stem cells to the ischemic cardiac tissue and promote heart repair. MDPI 2023-01-25 /pmc/articles/PMC9953248/ /pubmed/36830880 http://dx.doi.org/10.3390/biomedicines11020343 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bajdak-Rusinek, Karolina
Fus-Kujawa, Agnieszka
Buszman, Piotr
Żyła-Uklejewicz, Dorota
Jelonek, Katarzyna
Musiał-Kulik, Monika
Fernandez, Carlos
Michalak, Magdalena
George, Kurian
Kasperczyk, Janusz
Buszman, Paweł
SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction
title SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction
title_full SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction
title_fullStr SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction
title_full_unstemmed SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction
title_short SDF-1α-Releasing Microspheres Effectively Extend Stem Cell Homing after Myocardial Infarction
title_sort sdf-1α-releasing microspheres effectively extend stem cell homing after myocardial infarction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953248/
https://www.ncbi.nlm.nih.gov/pubmed/36830880
http://dx.doi.org/10.3390/biomedicines11020343
work_keys_str_mv AT bajdakrusinekkarolina sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT fuskujawaagnieszka sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT buszmanpiotr sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT zyłauklejewiczdorota sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT jelonekkatarzyna sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT musiałkulikmonika sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT fernandezcarlos sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT michalakmagdalena sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT georgekurian sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT kasperczykjanusz sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction
AT buszmanpaweł sdf1areleasingmicrosphereseffectivelyextendstemcellhomingaftermyocardialinfarction