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Two-Photon Excited Fluorescence of NADH-Alcohol Dehydrogenase Complex in a Mixture with Bacterial Enzymes

Thorough study of composition and fluorescence properties of a commercial reagent of active equine NAD-dependent alcohol dehydrogenase expressed and purified from E. coli has been carried out. Several experimental methods: spectral- and time-resolved two-photon excited fluorescence, sodium dodecyl s...

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Detalles Bibliográficos
Autores principales: Gorbunova, Ioanna A., Sasin, Maxim E., Yachkov, Dmitry V., Volkov, Denis A., Vedyaykin, Alexei D., Nikiforov, Andrey A., Vasyutinskii, Oleg S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953378/
https://www.ncbi.nlm.nih.gov/pubmed/36830625
http://dx.doi.org/10.3390/biom13020256