Establishment of an Integrated CRISPR/Cas9 Plasmid System for Simple and Efficient Genome Editing in Medaka In Vitro and In Vivo

SIMPLE SUMMARY: The application of CRISPR/Cas9 genome editing in fish is limited to date. An easy-to-use, economical and effective genome editing system is greatly needed. In this study, we established an integrated pCas9-U6sgRNA plasmid system, the sgRNA of which was driven by U6 promoters from dif...

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Detalles Bibliográficos
Autores principales: Zhang, Zeming, Wang, Jie, Li, Jianeng, Liu, Xiang, Liu, Lei, Zhao, Changle, Tao, Wenjing, Wang, Deshou, Wei, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953409/
https://www.ncbi.nlm.nih.gov/pubmed/36829610
http://dx.doi.org/10.3390/biology12020336

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953409/
https://www.ncbi.nlm.nih.gov/pubmed/36829610
http://dx.doi.org/10.3390/biology12020336

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