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Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat

Wearable biosensors have the potential for developing individualized health evaluation and detection systems owing to their ability to provide continuous real-time physiological data. Among various wearable biosensors, localized surface plasmon resonance (LSPR)-based wearable sensors can be versatil...

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Autores principales: Nan, Minghui, Darmawan, Bobby Aditya, Go, Gwangjun, Zheng, Shirong, Lee, Junhyeok, Kim, Seokjae, Lee, Taeksu, Choi, Eunpyo, Park, Jong-Oh, Bang, Doyeon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953546/
https://www.ncbi.nlm.nih.gov/pubmed/36831950
http://dx.doi.org/10.3390/bios13020184
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author Nan, Minghui
Darmawan, Bobby Aditya
Go, Gwangjun
Zheng, Shirong
Lee, Junhyeok
Kim, Seokjae
Lee, Taeksu
Choi, Eunpyo
Park, Jong-Oh
Bang, Doyeon
author_facet Nan, Minghui
Darmawan, Bobby Aditya
Go, Gwangjun
Zheng, Shirong
Lee, Junhyeok
Kim, Seokjae
Lee, Taeksu
Choi, Eunpyo
Park, Jong-Oh
Bang, Doyeon
author_sort Nan, Minghui
collection PubMed
description Wearable biosensors have the potential for developing individualized health evaluation and detection systems owing to their ability to provide continuous real-time physiological data. Among various wearable biosensors, localized surface plasmon resonance (LSPR)-based wearable sensors can be versatile in various practical applications owing to their sensitive interactions with specific analytes. Understanding and analyzing endocrine responses to stress is particularly crucial for evaluating human performance, diagnosing stress-related diseases, and monitoring mental health, as stress takes a serious toll on physiological health and psychological well-being. Cortisol is an essential biomarker of stress because of the close relationship between cortisol concentration in the human body and stress level. In this study, a flexible LSPR biosensor was manufactured to detect cortisol levels in the human body by depositing gold nanoparticle (AuNP) layers on a 3-aminopropyltriethoxysilane (APTES)-functionalized poly (dimethylsiloxane) (PDMS) substrate. Subsequently, an aptamer was immobilized on the surface of the LSPR substrate, enabling highly sensitive and selective cortisol capture owing to its specific cortisol recognition. The biosensor exhibited excellent detection ability in cortisol solutions of various concentrations ranging from 0.1 to 1000 nM with a detection limit of 0.1 nM. The flexible LSPR biosensor also demonstrated good stability under various mechanical deformations. Furthermore, the cortisol levels of the flexible LSPR biosensor were also measured in the human epidermis before and after exercise as well as in the morning and afternoon. Our biosensors, which combine easily manufactured flexible sensors with sensitive cortisol-detecting molecules to measure human stress levels, could be versatile candidates for human-friendly products.
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spelling pubmed-99535462023-02-25 Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat Nan, Minghui Darmawan, Bobby Aditya Go, Gwangjun Zheng, Shirong Lee, Junhyeok Kim, Seokjae Lee, Taeksu Choi, Eunpyo Park, Jong-Oh Bang, Doyeon Biosensors (Basel) Article Wearable biosensors have the potential for developing individualized health evaluation and detection systems owing to their ability to provide continuous real-time physiological data. Among various wearable biosensors, localized surface plasmon resonance (LSPR)-based wearable sensors can be versatile in various practical applications owing to their sensitive interactions with specific analytes. Understanding and analyzing endocrine responses to stress is particularly crucial for evaluating human performance, diagnosing stress-related diseases, and monitoring mental health, as stress takes a serious toll on physiological health and psychological well-being. Cortisol is an essential biomarker of stress because of the close relationship between cortisol concentration in the human body and stress level. In this study, a flexible LSPR biosensor was manufactured to detect cortisol levels in the human body by depositing gold nanoparticle (AuNP) layers on a 3-aminopropyltriethoxysilane (APTES)-functionalized poly (dimethylsiloxane) (PDMS) substrate. Subsequently, an aptamer was immobilized on the surface of the LSPR substrate, enabling highly sensitive and selective cortisol capture owing to its specific cortisol recognition. The biosensor exhibited excellent detection ability in cortisol solutions of various concentrations ranging from 0.1 to 1000 nM with a detection limit of 0.1 nM. The flexible LSPR biosensor also demonstrated good stability under various mechanical deformations. Furthermore, the cortisol levels of the flexible LSPR biosensor were also measured in the human epidermis before and after exercise as well as in the morning and afternoon. Our biosensors, which combine easily manufactured flexible sensors with sensitive cortisol-detecting molecules to measure human stress levels, could be versatile candidates for human-friendly products. MDPI 2023-01-24 /pmc/articles/PMC9953546/ /pubmed/36831950 http://dx.doi.org/10.3390/bios13020184 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nan, Minghui
Darmawan, Bobby Aditya
Go, Gwangjun
Zheng, Shirong
Lee, Junhyeok
Kim, Seokjae
Lee, Taeksu
Choi, Eunpyo
Park, Jong-Oh
Bang, Doyeon
Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat
title Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat
title_full Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat
title_fullStr Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat
title_full_unstemmed Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat
title_short Wearable Localized Surface Plasmon Resonance-Based Biosensor with Highly Sensitive and Direct Detection of Cortisol in Human Sweat
title_sort wearable localized surface plasmon resonance-based biosensor with highly sensitive and direct detection of cortisol in human sweat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953546/
https://www.ncbi.nlm.nih.gov/pubmed/36831950
http://dx.doi.org/10.3390/bios13020184
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