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Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate

SIMPLE SUMMARY: Hyperuricemia is a major risk factor for gout. Inhibition of liver xanthine oxidase has been shown to reduce the uric acid level in blood. However, side effects were reported for the xanthine oxidase inhibitors clinically used in the treatment of hyperuricemia. This study aimed to ev...

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Autores principales: Huang, Kuo-Ching, Chang, Yu-Ting, Pranata, Rosita, Cheng, Yung-Hsuan, Chen, Yu-Chi, Kuo, Ping-Chung, Huang, Yi-Hsuan, Tzen, Jason T. C., Chen, Rong-Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953564/
https://www.ncbi.nlm.nih.gov/pubmed/36829604
http://dx.doi.org/10.3390/biology12020329
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author Huang, Kuo-Ching
Chang, Yu-Ting
Pranata, Rosita
Cheng, Yung-Hsuan
Chen, Yu-Chi
Kuo, Ping-Chung
Huang, Yi-Hsuan
Tzen, Jason T. C.
Chen, Rong-Jane
author_facet Huang, Kuo-Ching
Chang, Yu-Ting
Pranata, Rosita
Cheng, Yung-Hsuan
Chen, Yu-Chi
Kuo, Ping-Chung
Huang, Yi-Hsuan
Tzen, Jason T. C.
Chen, Rong-Jane
author_sort Huang, Kuo-Ching
collection PubMed
description SIMPLE SUMMARY: Hyperuricemia is a major risk factor for gout. Inhibition of liver xanthine oxidase has been shown to reduce the uric acid level in blood. However, side effects were reported for the xanthine oxidase inhibitors clinically used in the treatment of hyperuricemia. This study aimed to evaluate strictinin, a natural polyphenol from Pu’er tea, for its therapeutic effects on hyperuricemia. Alleviation of hyperuricemia by strictinin supplementation was observed in AML12 mouse hepatocytes treated with xanthine as well as in an animal model using mice treated with potassium oxonate. ABSTRACT: Hyperuricemia, an abnormally high level of blood uric acid, is a major risk factor for gout. Although xanthine oxidase inhibitors were clinically used to lower blood uric acid level, the concerned side effects restricted their utilization. In this study, strictinin, an abundant polyphenol in Pu’er tea, was evaluated for its preventive effects on hyperuricemia. The results showed that the xanthine oxidase activity, uric acid production, and inflammation in AML12 mouse hepatocytes treated with xanthine were significantly reduced by the supplementation of strictinin. Detailed analyses revealed that strictinin inhibited xanthine-induced NLRP3 inflammasome activation. Consistently, the elevated blood uric acid level and the enhanced xanthine oxidase activity in mice treated with potassium oxonate were effectively diminished by strictinin supplementation. Moreover, for the first time, strictinin was found to promote healthy gut microbiota. Overall, strictinin possesses a great potential to be utilized as a functional ingredient for the prevention of hyperuricemia.
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spelling pubmed-99535642023-02-25 Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate Huang, Kuo-Ching Chang, Yu-Ting Pranata, Rosita Cheng, Yung-Hsuan Chen, Yu-Chi Kuo, Ping-Chung Huang, Yi-Hsuan Tzen, Jason T. C. Chen, Rong-Jane Biology (Basel) Article SIMPLE SUMMARY: Hyperuricemia is a major risk factor for gout. Inhibition of liver xanthine oxidase has been shown to reduce the uric acid level in blood. However, side effects were reported for the xanthine oxidase inhibitors clinically used in the treatment of hyperuricemia. This study aimed to evaluate strictinin, a natural polyphenol from Pu’er tea, for its therapeutic effects on hyperuricemia. Alleviation of hyperuricemia by strictinin supplementation was observed in AML12 mouse hepatocytes treated with xanthine as well as in an animal model using mice treated with potassium oxonate. ABSTRACT: Hyperuricemia, an abnormally high level of blood uric acid, is a major risk factor for gout. Although xanthine oxidase inhibitors were clinically used to lower blood uric acid level, the concerned side effects restricted their utilization. In this study, strictinin, an abundant polyphenol in Pu’er tea, was evaluated for its preventive effects on hyperuricemia. The results showed that the xanthine oxidase activity, uric acid production, and inflammation in AML12 mouse hepatocytes treated with xanthine were significantly reduced by the supplementation of strictinin. Detailed analyses revealed that strictinin inhibited xanthine-induced NLRP3 inflammasome activation. Consistently, the elevated blood uric acid level and the enhanced xanthine oxidase activity in mice treated with potassium oxonate were effectively diminished by strictinin supplementation. Moreover, for the first time, strictinin was found to promote healthy gut microbiota. Overall, strictinin possesses a great potential to be utilized as a functional ingredient for the prevention of hyperuricemia. MDPI 2023-02-17 /pmc/articles/PMC9953564/ /pubmed/36829604 http://dx.doi.org/10.3390/biology12020329 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Kuo-Ching
Chang, Yu-Ting
Pranata, Rosita
Cheng, Yung-Hsuan
Chen, Yu-Chi
Kuo, Ping-Chung
Huang, Yi-Hsuan
Tzen, Jason T. C.
Chen, Rong-Jane
Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate
title Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate
title_full Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate
title_fullStr Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate
title_full_unstemmed Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate
title_short Alleviation of Hyperuricemia by Strictinin in AML12 Mouse Hepatocytes Treated with Xanthine and in Mice Treated with Potassium Oxonate
title_sort alleviation of hyperuricemia by strictinin in aml12 mouse hepatocytes treated with xanthine and in mice treated with potassium oxonate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953564/
https://www.ncbi.nlm.nih.gov/pubmed/36829604
http://dx.doi.org/10.3390/biology12020329
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