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Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia

(1) Background: Information regarding gene expression profiles and the prognosis of community-acquired pneumonia (CAP) is scarce. We aimed to examine the differences in the gene expression profiles in peripheral blood at hospital admission between patients with CAP who died during hospitalization an...

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Autores principales: Viasus, Diego, Simonetti, Antonella F., Nonell, Lara, Vidal, Oscar, Meije, Yolanda, Ortega, Lucía, Arnal, Magdalena, Bódalo-Torruella, Marta, Sierra, Montserrat, Rombauts, Alexander, Abelenda-Alonso, Gabriela, Blanchart, Gemma, Gudiol, Carlota, Carratalà, Jordi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953679/
https://www.ncbi.nlm.nih.gov/pubmed/36830965
http://dx.doi.org/10.3390/biomedicines11020429
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author Viasus, Diego
Simonetti, Antonella F.
Nonell, Lara
Vidal, Oscar
Meije, Yolanda
Ortega, Lucía
Arnal, Magdalena
Bódalo-Torruella, Marta
Sierra, Montserrat
Rombauts, Alexander
Abelenda-Alonso, Gabriela
Blanchart, Gemma
Gudiol, Carlota
Carratalà, Jordi
author_facet Viasus, Diego
Simonetti, Antonella F.
Nonell, Lara
Vidal, Oscar
Meije, Yolanda
Ortega, Lucía
Arnal, Magdalena
Bódalo-Torruella, Marta
Sierra, Montserrat
Rombauts, Alexander
Abelenda-Alonso, Gabriela
Blanchart, Gemma
Gudiol, Carlota
Carratalà, Jordi
author_sort Viasus, Diego
collection PubMed
description (1) Background: Information regarding gene expression profiles and the prognosis of community-acquired pneumonia (CAP) is scarce. We aimed to examine the differences in the gene expression profiles in peripheral blood at hospital admission between patients with CAP who died during hospitalization and those who survived. (2) Methods: This is a multicenter study of nonimmunosuppressed adult patients who required hospitalization for CAP. Whole blood samples were obtained within 24 h of admission for genome-expression-profile analysis. Gene expression profiling identified both differentially expressed genes and enriched gene sets. (3) Results: A total of 198 samples from adult patients who required hospitalization for CAP were processed, of which 13 were from patients who died. Comparison of gene expression between patients who died and those who survived yielded 49 differentially expressed genes, 36 of which were upregulated and 13 downregulated. Gene set enrichment analysis (GSEA) identified four positively enriched gene sets in survivors, mainly associated with the interferon-alpha response, apoptosis, and sex hormone pathways. Similarly, GSEA identified seven positively enriched gene sets, associated with the oxidative stress, endoplasmic reticulum stress, oxidative phosphorylation, and angiogenesis pathways, in the patients who died. Protein–protein-interaction-network analysis identified FOS, CDC42, SLC26A10, EIF4G2, CCND3, ASXL1, UBE2S, and AURKA as the main gene hubs. (4) Conclusions: We found differences in gene expression profiles at hospital admission between CAP patients who died and those who survived. Our findings may help to identify novel candidate pathways and targets for potential intervention and biomarkers for risk stratification.
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spelling pubmed-99536792023-02-25 Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia Viasus, Diego Simonetti, Antonella F. Nonell, Lara Vidal, Oscar Meije, Yolanda Ortega, Lucía Arnal, Magdalena Bódalo-Torruella, Marta Sierra, Montserrat Rombauts, Alexander Abelenda-Alonso, Gabriela Blanchart, Gemma Gudiol, Carlota Carratalà, Jordi Biomedicines Article (1) Background: Information regarding gene expression profiles and the prognosis of community-acquired pneumonia (CAP) is scarce. We aimed to examine the differences in the gene expression profiles in peripheral blood at hospital admission between patients with CAP who died during hospitalization and those who survived. (2) Methods: This is a multicenter study of nonimmunosuppressed adult patients who required hospitalization for CAP. Whole blood samples were obtained within 24 h of admission for genome-expression-profile analysis. Gene expression profiling identified both differentially expressed genes and enriched gene sets. (3) Results: A total of 198 samples from adult patients who required hospitalization for CAP were processed, of which 13 were from patients who died. Comparison of gene expression between patients who died and those who survived yielded 49 differentially expressed genes, 36 of which were upregulated and 13 downregulated. Gene set enrichment analysis (GSEA) identified four positively enriched gene sets in survivors, mainly associated with the interferon-alpha response, apoptosis, and sex hormone pathways. Similarly, GSEA identified seven positively enriched gene sets, associated with the oxidative stress, endoplasmic reticulum stress, oxidative phosphorylation, and angiogenesis pathways, in the patients who died. Protein–protein-interaction-network analysis identified FOS, CDC42, SLC26A10, EIF4G2, CCND3, ASXL1, UBE2S, and AURKA as the main gene hubs. (4) Conclusions: We found differences in gene expression profiles at hospital admission between CAP patients who died and those who survived. Our findings may help to identify novel candidate pathways and targets for potential intervention and biomarkers for risk stratification. MDPI 2023-02-01 /pmc/articles/PMC9953679/ /pubmed/36830965 http://dx.doi.org/10.3390/biomedicines11020429 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Viasus, Diego
Simonetti, Antonella F.
Nonell, Lara
Vidal, Oscar
Meije, Yolanda
Ortega, Lucía
Arnal, Magdalena
Bódalo-Torruella, Marta
Sierra, Montserrat
Rombauts, Alexander
Abelenda-Alonso, Gabriela
Blanchart, Gemma
Gudiol, Carlota
Carratalà, Jordi
Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia
title Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia
title_full Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia
title_fullStr Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia
title_full_unstemmed Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia
title_short Whole-Blood Gene Expression Profiles Associated with Mortality in Community-Acquired Pneumonia
title_sort whole-blood gene expression profiles associated with mortality in community-acquired pneumonia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953679/
https://www.ncbi.nlm.nih.gov/pubmed/36830965
http://dx.doi.org/10.3390/biomedicines11020429
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