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Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation

Quorum-sensing peptides (QSPs) are bacterial peptides traditionally considered only as inter-bacterial communication molecules. Recently, their involvement in microbiome–host interactions influencing host diseases such as cancer and sarcopenia were explored. However, it is still unknown to what exte...

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Autores principales: De Spiegeleer, Anton, Descamps, Amélie, Govindarajan, Srinath, Coudenys, Julie, Van der borght, Kevin, Hirmz, Hannah, Van Den Noortgate, Nele, Elewaut, Dirk, De Spiegeleer, Bart, Wynendaele, Evelien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953703/
https://www.ncbi.nlm.nih.gov/pubmed/36830664
http://dx.doi.org/10.3390/biom13020296
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author De Spiegeleer, Anton
Descamps, Amélie
Govindarajan, Srinath
Coudenys, Julie
Van der borght, Kevin
Hirmz, Hannah
Van Den Noortgate, Nele
Elewaut, Dirk
De Spiegeleer, Bart
Wynendaele, Evelien
author_facet De Spiegeleer, Anton
Descamps, Amélie
Govindarajan, Srinath
Coudenys, Julie
Van der borght, Kevin
Hirmz, Hannah
Van Den Noortgate, Nele
Elewaut, Dirk
De Spiegeleer, Bart
Wynendaele, Evelien
author_sort De Spiegeleer, Anton
collection PubMed
description Quorum-sensing peptides (QSPs) are bacterial peptides traditionally considered only as inter-bacterial communication molecules. Recently, their involvement in microbiome–host interactions influencing host diseases such as cancer and sarcopenia were explored. However, it is still unknown to what extent these peptides have the potential to modulate the immune system. In this proof-of-concept study, we screened 89 QSPs for their potential to induce IL-6 and TNFα in murine splenocytes and J774 macrophages. Confirmatory experiments on the positive screening-hits were conducted using murine splenocytes and human PBMCs of different ages. Finally, to investigate the biological relevance of immunomodulatory QSPs, we analysed plasma in a human cohort for the presence of the immunomodulatory QSP Q010. To do this, we used a newly developed UHPLC-MS/MS method. Our findings indicated that specific QSPs activate immune cells in vitro, with Q007, Q010, Q017 and Q212 being the top four screening hits. Q007 and Q010 were affirmed in subsequent confirmatory experiments using murine splenocytes and human PBMCs. Finally, Q010 was detected in human plasma, demonstrating for the first time the presence of an immunomodulatory QSP in human circulation. In conclusion, our data are the first evidence indicating the potential of biologically relevant quorum-sensing peptides to modulate the immune system.
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spelling pubmed-99537032023-02-25 Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation De Spiegeleer, Anton Descamps, Amélie Govindarajan, Srinath Coudenys, Julie Van der borght, Kevin Hirmz, Hannah Van Den Noortgate, Nele Elewaut, Dirk De Spiegeleer, Bart Wynendaele, Evelien Biomolecules Article Quorum-sensing peptides (QSPs) are bacterial peptides traditionally considered only as inter-bacterial communication molecules. Recently, their involvement in microbiome–host interactions influencing host diseases such as cancer and sarcopenia were explored. However, it is still unknown to what extent these peptides have the potential to modulate the immune system. In this proof-of-concept study, we screened 89 QSPs for their potential to induce IL-6 and TNFα in murine splenocytes and J774 macrophages. Confirmatory experiments on the positive screening-hits were conducted using murine splenocytes and human PBMCs of different ages. Finally, to investigate the biological relevance of immunomodulatory QSPs, we analysed plasma in a human cohort for the presence of the immunomodulatory QSP Q010. To do this, we used a newly developed UHPLC-MS/MS method. Our findings indicated that specific QSPs activate immune cells in vitro, with Q007, Q010, Q017 and Q212 being the top four screening hits. Q007 and Q010 were affirmed in subsequent confirmatory experiments using murine splenocytes and human PBMCs. Finally, Q010 was detected in human plasma, demonstrating for the first time the presence of an immunomodulatory QSP in human circulation. In conclusion, our data are the first evidence indicating the potential of biologically relevant quorum-sensing peptides to modulate the immune system. MDPI 2023-02-04 /pmc/articles/PMC9953703/ /pubmed/36830664 http://dx.doi.org/10.3390/biom13020296 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Spiegeleer, Anton
Descamps, Amélie
Govindarajan, Srinath
Coudenys, Julie
Van der borght, Kevin
Hirmz, Hannah
Van Den Noortgate, Nele
Elewaut, Dirk
De Spiegeleer, Bart
Wynendaele, Evelien
Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation
title Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation
title_full Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation
title_fullStr Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation
title_full_unstemmed Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation
title_short Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation
title_sort bacterial quorum-sensing peptides as immune modulators present in systemic circulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953703/
https://www.ncbi.nlm.nih.gov/pubmed/36830664
http://dx.doi.org/10.3390/biom13020296
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