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Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy

MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact de...

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Autores principales: Yuehui, Zhang, Hosoki, Maki, Oshima, Masamitsu, Tajima, Toyoko, Miyagi, Mayu, Raman, Swarnalakshmi, Raju, Resmi, Matsuka, Yoshizo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953729/
https://www.ncbi.nlm.nih.gov/pubmed/36830814
http://dx.doi.org/10.3390/biomedicines11020277
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author Yuehui, Zhang
Hosoki, Maki
Oshima, Masamitsu
Tajima, Toyoko
Miyagi, Mayu
Raman, Swarnalakshmi
Raju, Resmi
Matsuka, Yoshizo
author_facet Yuehui, Zhang
Hosoki, Maki
Oshima, Masamitsu
Tajima, Toyoko
Miyagi, Mayu
Raman, Swarnalakshmi
Raju, Resmi
Matsuka, Yoshizo
author_sort Yuehui, Zhang
collection PubMed
description MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (n = 3) were enrolled along with additional female participants with no medical history of metal allergy (n = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies.
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spelling pubmed-99537292023-02-25 Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy Yuehui, Zhang Hosoki, Maki Oshima, Masamitsu Tajima, Toyoko Miyagi, Mayu Raman, Swarnalakshmi Raju, Resmi Matsuka, Yoshizo Biomedicines Article MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (n = 3) were enrolled along with additional female participants with no medical history of metal allergy (n = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies. MDPI 2023-01-19 /pmc/articles/PMC9953729/ /pubmed/36830814 http://dx.doi.org/10.3390/biomedicines11020277 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuehui, Zhang
Hosoki, Maki
Oshima, Masamitsu
Tajima, Toyoko
Miyagi, Mayu
Raman, Swarnalakshmi
Raju, Resmi
Matsuka, Yoshizo
Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
title Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
title_full Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
title_fullStr Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
title_full_unstemmed Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
title_short Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
title_sort identification of microrna signatures in peripheral blood of young women as potential biomarkers for metal allergy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953729/
https://www.ncbi.nlm.nih.gov/pubmed/36830814
http://dx.doi.org/10.3390/biomedicines11020277
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