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Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact de...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953729/ https://www.ncbi.nlm.nih.gov/pubmed/36830814 http://dx.doi.org/10.3390/biomedicines11020277 |
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author | Yuehui, Zhang Hosoki, Maki Oshima, Masamitsu Tajima, Toyoko Miyagi, Mayu Raman, Swarnalakshmi Raju, Resmi Matsuka, Yoshizo |
author_facet | Yuehui, Zhang Hosoki, Maki Oshima, Masamitsu Tajima, Toyoko Miyagi, Mayu Raman, Swarnalakshmi Raju, Resmi Matsuka, Yoshizo |
author_sort | Yuehui, Zhang |
collection | PubMed |
description | MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (n = 3) were enrolled along with additional female participants with no medical history of metal allergy (n = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies. |
format | Online Article Text |
id | pubmed-9953729 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-99537292023-02-25 Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy Yuehui, Zhang Hosoki, Maki Oshima, Masamitsu Tajima, Toyoko Miyagi, Mayu Raman, Swarnalakshmi Raju, Resmi Matsuka, Yoshizo Biomedicines Article MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (n = 3) were enrolled along with additional female participants with no medical history of metal allergy (n = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies. MDPI 2023-01-19 /pmc/articles/PMC9953729/ /pubmed/36830814 http://dx.doi.org/10.3390/biomedicines11020277 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yuehui, Zhang Hosoki, Maki Oshima, Masamitsu Tajima, Toyoko Miyagi, Mayu Raman, Swarnalakshmi Raju, Resmi Matsuka, Yoshizo Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
title | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
title_full | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
title_fullStr | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
title_full_unstemmed | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
title_short | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
title_sort | identification of microrna signatures in peripheral blood of young women as potential biomarkers for metal allergy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953729/ https://www.ncbi.nlm.nih.gov/pubmed/36830814 http://dx.doi.org/10.3390/biomedicines11020277 |
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