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CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective

A ratiometric fluorescence probe based on the conjugation of peroxyfluor-NHS (PF) and carbon dots (CDs) was designed for selective and rapid detection of glucose. When glucose was catalytically oxidized by glucose oxidase (GOx), the product H(2)O(2) would react with colorless and non-fluorescent per...

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Autores principales: Li, Yangjie, Luo, Site, Wang, Xin, He, Yang, Yu, Haihu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953814/
https://www.ncbi.nlm.nih.gov/pubmed/36831988
http://dx.doi.org/10.3390/bios13020222
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author Li, Yangjie
Luo, Site
Wang, Xin
He, Yang
Yu, Haihu
author_facet Li, Yangjie
Luo, Site
Wang, Xin
He, Yang
Yu, Haihu
author_sort Li, Yangjie
collection PubMed
description A ratiometric fluorescence probe based on the conjugation of peroxyfluor-NHS (PF) and carbon dots (CDs) was designed for selective and rapid detection of glucose. When glucose was catalytically oxidized by glucose oxidase (GOx), the product H(2)O(2) would react with colorless and non-fluorescent peroxyfluor moiety to give the colored and fluorescent fluorescein moiety which would absorb the energy of CDs emission at 450 nm due to the Förster Resonance Energy Transfer (FRET) and generate a new emission peak at 517 nm. The reaction between PF and H(2)O(2) was slow with a rate constant of about 2.7 × 10(−4) s(−1) under pseudo-first-order conditions (1 uM PF, 1 mM H(2)O(2)), which was unconducive to rapid detection. Given this, a short time detection method was proposed by studying the kinetics of the reaction between PF and H(2)O(2). In this method, the detection time was fixed at three minutes. The linear detection of glucose could be well realized even if the reaction was partially done. As glucose concentration increased from 0.05 mM to 5 mM, the fluorescence intensity ratio (I(517)/I(450)) after 3 minutes’ reaction of CDs-PF and glucose oxidation products changed linearly from 0.269 to 1.127 with the limit of detection (LOD) of 17.19 μM. In addition, the applicability of the probe in blood glucose detection was verified.
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spelling pubmed-99538142023-02-25 CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective Li, Yangjie Luo, Site Wang, Xin He, Yang Yu, Haihu Biosensors (Basel) Article A ratiometric fluorescence probe based on the conjugation of peroxyfluor-NHS (PF) and carbon dots (CDs) was designed for selective and rapid detection of glucose. When glucose was catalytically oxidized by glucose oxidase (GOx), the product H(2)O(2) would react with colorless and non-fluorescent peroxyfluor moiety to give the colored and fluorescent fluorescein moiety which would absorb the energy of CDs emission at 450 nm due to the Förster Resonance Energy Transfer (FRET) and generate a new emission peak at 517 nm. The reaction between PF and H(2)O(2) was slow with a rate constant of about 2.7 × 10(−4) s(−1) under pseudo-first-order conditions (1 uM PF, 1 mM H(2)O(2)), which was unconducive to rapid detection. Given this, a short time detection method was proposed by studying the kinetics of the reaction between PF and H(2)O(2). In this method, the detection time was fixed at three minutes. The linear detection of glucose could be well realized even if the reaction was partially done. As glucose concentration increased from 0.05 mM to 5 mM, the fluorescence intensity ratio (I(517)/I(450)) after 3 minutes’ reaction of CDs-PF and glucose oxidation products changed linearly from 0.269 to 1.127 with the limit of detection (LOD) of 17.19 μM. In addition, the applicability of the probe in blood glucose detection was verified. MDPI 2023-02-03 /pmc/articles/PMC9953814/ /pubmed/36831988 http://dx.doi.org/10.3390/bios13020222 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yangjie
Luo, Site
Wang, Xin
He, Yang
Yu, Haihu
CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective
title CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective
title_full CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective
title_fullStr CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective
title_full_unstemmed CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective
title_short CDs-Peroxyfluor Conjugation for Ratiometric Fluorescence Detection of Glucose and Shortening Its Detection Time from Reaction Dynamic Perspective
title_sort cds-peroxyfluor conjugation for ratiometric fluorescence detection of glucose and shortening its detection time from reaction dynamic perspective
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953814/
https://www.ncbi.nlm.nih.gov/pubmed/36831988
http://dx.doi.org/10.3390/bios13020222
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