Cargando…

Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder

Elevated levels of histamine cause over-secretion of gastric hydrochloric acid (HCl), leading to gastrointestinal (GI) disorders and anxiety. Ranitidine is an antihistamine drug widely used in the management of GI disorders, as it works by blocking the histamine−2 receptors in parietal cells, thereb...

Descripción completa

Detalles Bibliográficos
Autores principales: Selvaraj, Divya Bharathi, Vergil Andrews, Jemi Feiona, Anusuyadevi, Muthuswamy, Kandasamy, Mahesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953842/
https://www.ncbi.nlm.nih.gov/pubmed/36831809
http://dx.doi.org/10.3390/brainsci13020266
_version_ 1784893978095648768
author Selvaraj, Divya Bharathi
Vergil Andrews, Jemi Feiona
Anusuyadevi, Muthuswamy
Kandasamy, Mahesh
author_facet Selvaraj, Divya Bharathi
Vergil Andrews, Jemi Feiona
Anusuyadevi, Muthuswamy
Kandasamy, Mahesh
author_sort Selvaraj, Divya Bharathi
collection PubMed
description Elevated levels of histamine cause over-secretion of gastric hydrochloric acid (HCl), leading to gastrointestinal (GI) disorders and anxiety. Ranitidine is an antihistamine drug widely used in the management of GI disorders, as it works by blocking the histamine−2 receptors in parietal cells, thereby reducing the production of HCl in the stomach. While some reports indicate the neuroprotective effects of ranitidine, its role against GI disorder-related anxiety remains unclear. Therefore, we investigated the effect of ranitidine against anxiety-related behaviors in association with changes in neuronal density in the hippocampal cornu ammonis (CA)–3 region of cysteamine hydrochloride-induced mouse model of GI disorder. Results obtained from the open field test (OFT), light and dark box test (LDBT), and elevated plus maze (EPM) test revealed that ranitidine treatment reduces anxiety-like behaviors in experimental animals. Nissl staining and immunohistochemical assessment of ionized calcium-binding adapter molecule (Iba)-1 positive microglia in cryosectioned brains indicated enhanced density of pyramidal neurons and reduced activation of microglia in the hippocampal CA–3 region of brains of ranitidine-treated experimental mice. Therefore, this study suggests that ranitidine mediates anxiolytic effects, which can be translated to establish a pharmacological regime to ameliorate anxiety-related symptoms in humans.
format Online
Article
Text
id pubmed-9953842
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99538422023-02-25 Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder Selvaraj, Divya Bharathi Vergil Andrews, Jemi Feiona Anusuyadevi, Muthuswamy Kandasamy, Mahesh Brain Sci Article Elevated levels of histamine cause over-secretion of gastric hydrochloric acid (HCl), leading to gastrointestinal (GI) disorders and anxiety. Ranitidine is an antihistamine drug widely used in the management of GI disorders, as it works by blocking the histamine−2 receptors in parietal cells, thereby reducing the production of HCl in the stomach. While some reports indicate the neuroprotective effects of ranitidine, its role against GI disorder-related anxiety remains unclear. Therefore, we investigated the effect of ranitidine against anxiety-related behaviors in association with changes in neuronal density in the hippocampal cornu ammonis (CA)–3 region of cysteamine hydrochloride-induced mouse model of GI disorder. Results obtained from the open field test (OFT), light and dark box test (LDBT), and elevated plus maze (EPM) test revealed that ranitidine treatment reduces anxiety-like behaviors in experimental animals. Nissl staining and immunohistochemical assessment of ionized calcium-binding adapter molecule (Iba)-1 positive microglia in cryosectioned brains indicated enhanced density of pyramidal neurons and reduced activation of microglia in the hippocampal CA–3 region of brains of ranitidine-treated experimental mice. Therefore, this study suggests that ranitidine mediates anxiolytic effects, which can be translated to establish a pharmacological regime to ameliorate anxiety-related symptoms in humans. MDPI 2023-02-04 /pmc/articles/PMC9953842/ /pubmed/36831809 http://dx.doi.org/10.3390/brainsci13020266 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Selvaraj, Divya Bharathi
Vergil Andrews, Jemi Feiona
Anusuyadevi, Muthuswamy
Kandasamy, Mahesh
Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder
title Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder
title_full Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder
title_fullStr Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder
title_full_unstemmed Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder
title_short Ranitidine Alleviates Anxiety-like Behaviors and Improves the Density of Pyramidal Neurons upon Deactivation of Microglia in the CA3 Region of the Hippocampus in a Cysteamine HCl-Induced Mouse Model of Gastrointestinal Disorder
title_sort ranitidine alleviates anxiety-like behaviors and improves the density of pyramidal neurons upon deactivation of microglia in the ca3 region of the hippocampus in a cysteamine hcl-induced mouse model of gastrointestinal disorder
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953842/
https://www.ncbi.nlm.nih.gov/pubmed/36831809
http://dx.doi.org/10.3390/brainsci13020266
work_keys_str_mv AT selvarajdivyabharathi ranitidinealleviatesanxietylikebehaviorsandimprovesthedensityofpyramidalneuronsupondeactivationofmicrogliaintheca3regionofthehippocampusinacysteaminehclinducedmousemodelofgastrointestinaldisorder
AT vergilandrewsjemifeiona ranitidinealleviatesanxietylikebehaviorsandimprovesthedensityofpyramidalneuronsupondeactivationofmicrogliaintheca3regionofthehippocampusinacysteaminehclinducedmousemodelofgastrointestinaldisorder
AT anusuyadevimuthuswamy ranitidinealleviatesanxietylikebehaviorsandimprovesthedensityofpyramidalneuronsupondeactivationofmicrogliaintheca3regionofthehippocampusinacysteaminehclinducedmousemodelofgastrointestinaldisorder
AT kandasamymahesh ranitidinealleviatesanxietylikebehaviorsandimprovesthedensityofpyramidalneuronsupondeactivationofmicrogliaintheca3regionofthehippocampusinacysteaminehclinducedmousemodelofgastrointestinaldisorder