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MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine

Marine invertebrates are multicellular organisms consisting of a wide range of marine environmental species. Unlike vertebrates, including humans, one of the challenges in identifying and tracking invertebrate stem cells is the lack of a specific marker. Labeling stem cells with magnetic particles p...

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Autores principales: Baghban, Neda, Khoradmehr, Arezoo, Afshar, Alireza, Jafari, Nazanin, Zendehboudi, Tuba, Rasekh, Poorya, Abolfathi, Leila Gholamian, Barmak, Alireza, Mohebbi, Gholamhossein, Akmaral, Baspakova, Askerovich, Kaliyev Asset, Maratovich, Mussin Nadiar, Azari, Hossein, Assadi, Majid, Nabipour, Iraj, Tamadon, Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953982/
https://www.ncbi.nlm.nih.gov/pubmed/36832034
http://dx.doi.org/10.3390/bios13020268
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author Baghban, Neda
Khoradmehr, Arezoo
Afshar, Alireza
Jafari, Nazanin
Zendehboudi, Tuba
Rasekh, Poorya
Abolfathi, Leila Gholamian
Barmak, Alireza
Mohebbi, Gholamhossein
Akmaral, Baspakova
Askerovich, Kaliyev Asset
Maratovich, Mussin Nadiar
Azari, Hossein
Assadi, Majid
Nabipour, Iraj
Tamadon, Amin
author_facet Baghban, Neda
Khoradmehr, Arezoo
Afshar, Alireza
Jafari, Nazanin
Zendehboudi, Tuba
Rasekh, Poorya
Abolfathi, Leila Gholamian
Barmak, Alireza
Mohebbi, Gholamhossein
Akmaral, Baspakova
Askerovich, Kaliyev Asset
Maratovich, Mussin Nadiar
Azari, Hossein
Assadi, Majid
Nabipour, Iraj
Tamadon, Amin
author_sort Baghban, Neda
collection PubMed
description Marine invertebrates are multicellular organisms consisting of a wide range of marine environmental species. Unlike vertebrates, including humans, one of the challenges in identifying and tracking invertebrate stem cells is the lack of a specific marker. Labeling stem cells with magnetic particles provides a non-invasive, in vivo tracking method using MRI. This study suggests antibody-conjugated iron nanoparticles (NPs), which are detectable with MRI for in vivo tracking, to detect stem cell proliferation using the Oct4 receptor as a marker of stem cells. In the first phase, iron NPs were fabricated, and their successful synthesis was confirmed using FTIR spectroscopy. Next, the Alexa Fluor anti-Oct4 antibody was conjugated with as-synthesized NPs. Their affinity to the cell surface marker in fresh and saltwater conditions was confirmed using two types of cells, murine mesenchymal stromal/stem cell culture and sea anemone stem cells. For this purpose, 106 cells of each type were exposed to NP-conjugated antibodies and their affinity to antibodies was confirmed by an epi-fluorescent microscope. The presence of iron-NPs imaged with the light microscope was confirmed by iron staining using Prussian blue stain. Next, anti-Oct4 antibodies conjugated with iron NPs were injected into a brittle star, and proliferating cells were tracked by MRI. To sum up, anti-Oct4 antibodies conjugated with iron NPs not only have the potential for identifying proliferating stem cells in different cell culture conditions of sea anemone and mouse cell cultures but also has the potential to be used for in vivo MRI tracking of marine proliferating cells.
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spelling pubmed-99539822023-02-25 MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine Baghban, Neda Khoradmehr, Arezoo Afshar, Alireza Jafari, Nazanin Zendehboudi, Tuba Rasekh, Poorya Abolfathi, Leila Gholamian Barmak, Alireza Mohebbi, Gholamhossein Akmaral, Baspakova Askerovich, Kaliyev Asset Maratovich, Mussin Nadiar Azari, Hossein Assadi, Majid Nabipour, Iraj Tamadon, Amin Biosensors (Basel) Article Marine invertebrates are multicellular organisms consisting of a wide range of marine environmental species. Unlike vertebrates, including humans, one of the challenges in identifying and tracking invertebrate stem cells is the lack of a specific marker. Labeling stem cells with magnetic particles provides a non-invasive, in vivo tracking method using MRI. This study suggests antibody-conjugated iron nanoparticles (NPs), which are detectable with MRI for in vivo tracking, to detect stem cell proliferation using the Oct4 receptor as a marker of stem cells. In the first phase, iron NPs were fabricated, and their successful synthesis was confirmed using FTIR spectroscopy. Next, the Alexa Fluor anti-Oct4 antibody was conjugated with as-synthesized NPs. Their affinity to the cell surface marker in fresh and saltwater conditions was confirmed using two types of cells, murine mesenchymal stromal/stem cell culture and sea anemone stem cells. For this purpose, 106 cells of each type were exposed to NP-conjugated antibodies and their affinity to antibodies was confirmed by an epi-fluorescent microscope. The presence of iron-NPs imaged with the light microscope was confirmed by iron staining using Prussian blue stain. Next, anti-Oct4 antibodies conjugated with iron NPs were injected into a brittle star, and proliferating cells were tracked by MRI. To sum up, anti-Oct4 antibodies conjugated with iron NPs not only have the potential for identifying proliferating stem cells in different cell culture conditions of sea anemone and mouse cell cultures but also has the potential to be used for in vivo MRI tracking of marine proliferating cells. MDPI 2023-02-13 /pmc/articles/PMC9953982/ /pubmed/36832034 http://dx.doi.org/10.3390/bios13020268 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baghban, Neda
Khoradmehr, Arezoo
Afshar, Alireza
Jafari, Nazanin
Zendehboudi, Tuba
Rasekh, Poorya
Abolfathi, Leila Gholamian
Barmak, Alireza
Mohebbi, Gholamhossein
Akmaral, Baspakova
Askerovich, Kaliyev Asset
Maratovich, Mussin Nadiar
Azari, Hossein
Assadi, Majid
Nabipour, Iraj
Tamadon, Amin
MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine
title MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine
title_full MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine
title_fullStr MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine
title_full_unstemmed MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine
title_short MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine
title_sort mri tracking of marine proliferating cells in vivo using anti-oct4 antibody-conjugated iron nanoparticles for precision in regenerative medicine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953982/
https://www.ncbi.nlm.nih.gov/pubmed/36832034
http://dx.doi.org/10.3390/bios13020268
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