Surface Plasmon Resonance Microscopy Based on Total Internal Reflection

Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a...

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Detalles Bibliográficos
Autores principales: Zhang, Teliang, Wang, Xueliang, Zeng, Youjun, Huang, Songfeng, Dai, Xiaoqi, Kong, Weifu, Liu, Qian, Chen, Jiajie, Qu, Junle, Shao, Yonghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9953993/
https://www.ncbi.nlm.nih.gov/pubmed/36832026
http://dx.doi.org/10.3390/bios13020261
Descripción
Sumario:Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.

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