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A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems

Cardiomyocyte alignment in myocardium tissue plays a significant role in the physiological, electrical, and mechanical functions of the myocardium. It remains, however, difficult to align cardiac cells in a 3D in vitro heart model. This paper proposes a simple method to align cells using microfabric...

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Autores principales: Navaee, Fatemeh, Khornian, Niloofar, Longet, David, Heub, Sarah, Boder-Pasche, Stephanie, Weder, Gilles, Kleger, Alexander, Renaud, Philippe, Braschler, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954012/
https://www.ncbi.nlm.nih.gov/pubmed/36831243
http://dx.doi.org/10.3390/cells12040576
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author Navaee, Fatemeh
Khornian, Niloofar
Longet, David
Heub, Sarah
Boder-Pasche, Stephanie
Weder, Gilles
Kleger, Alexander
Renaud, Philippe
Braschler, Thomas
author_facet Navaee, Fatemeh
Khornian, Niloofar
Longet, David
Heub, Sarah
Boder-Pasche, Stephanie
Weder, Gilles
Kleger, Alexander
Renaud, Philippe
Braschler, Thomas
author_sort Navaee, Fatemeh
collection PubMed
description Cardiomyocyte alignment in myocardium tissue plays a significant role in the physiological, electrical, and mechanical functions of the myocardium. It remains, however, difficult to align cardiac cells in a 3D in vitro heart model. This paper proposes a simple method to align cells using microfabricated Polydimethylsiloxane (PDMS) grooves with large dimensions (of up to 350 µm in width), similar to the dimensions of trabeculae carneae, the smallest functional unit of the myocardium. Two cell groups were used in this work; first, H9c2 cells in combination with Nor10 cells for proof of concept, and second, neonatal cardiac cells to investigate the functionality of the 3D model. This model compared the patterned and nonpatterned 3D constructs, as well as the 2D cell cultures, with and without patterns. In addition to alignment, we assessed the functionality of our proposed 3D model by comparing beating rates between aligned and non-aligned structures. In order to assess the practicality of the model, the 3D aligned structures should be demonstrated to be detachable and alignable. This evaluation is crucial to the use of this 3D functional model in future studies related to drug screening, building blocks for tissue engineering, and as a heart-on-chip by integrating microfluidics.
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spelling pubmed-99540122023-02-25 A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems Navaee, Fatemeh Khornian, Niloofar Longet, David Heub, Sarah Boder-Pasche, Stephanie Weder, Gilles Kleger, Alexander Renaud, Philippe Braschler, Thomas Cells Article Cardiomyocyte alignment in myocardium tissue plays a significant role in the physiological, electrical, and mechanical functions of the myocardium. It remains, however, difficult to align cardiac cells in a 3D in vitro heart model. This paper proposes a simple method to align cells using microfabricated Polydimethylsiloxane (PDMS) grooves with large dimensions (of up to 350 µm in width), similar to the dimensions of trabeculae carneae, the smallest functional unit of the myocardium. Two cell groups were used in this work; first, H9c2 cells in combination with Nor10 cells for proof of concept, and second, neonatal cardiac cells to investigate the functionality of the 3D model. This model compared the patterned and nonpatterned 3D constructs, as well as the 2D cell cultures, with and without patterns. In addition to alignment, we assessed the functionality of our proposed 3D model by comparing beating rates between aligned and non-aligned structures. In order to assess the practicality of the model, the 3D aligned structures should be demonstrated to be detachable and alignable. This evaluation is crucial to the use of this 3D functional model in future studies related to drug screening, building blocks for tissue engineering, and as a heart-on-chip by integrating microfluidics. MDPI 2023-02-10 /pmc/articles/PMC9954012/ /pubmed/36831243 http://dx.doi.org/10.3390/cells12040576 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Navaee, Fatemeh
Khornian, Niloofar
Longet, David
Heub, Sarah
Boder-Pasche, Stephanie
Weder, Gilles
Kleger, Alexander
Renaud, Philippe
Braschler, Thomas
A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems
title A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems
title_full A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems
title_fullStr A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems
title_full_unstemmed A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems
title_short A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems
title_sort three-dimensional engineered cardiac in vitro model: controlled alignment of cardiomyocytes in 3d microphysiological systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954012/
https://www.ncbi.nlm.nih.gov/pubmed/36831243
http://dx.doi.org/10.3390/cells12040576
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