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Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets

During aging, changes in gene expression are associated with a decline in physical and cognitive abilities. Here, we investigate the connection between changes in mRNA and protein expression in the brain by comparing the transcriptome and proteome of the mouse cortex during aging. Our transcriptomic...

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Autores principales: Khatir, Inès, Brunet, Marie A., Meller, Anna, Amiot, Florent, Patel, Tushar, Lapointe, Xavier, Avila Lopez, Jessica, Guilloy, Noé, Castonguay, Anne, Husain, Mohammed Amir, Germain, Joannie St., Boisvert, François-Michel, Plourde, Mélanie, Roucou, Xavier, Laurent, Benoit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954025/
https://www.ncbi.nlm.nih.gov/pubmed/36831282
http://dx.doi.org/10.3390/cells12040615
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author Khatir, Inès
Brunet, Marie A.
Meller, Anna
Amiot, Florent
Patel, Tushar
Lapointe, Xavier
Avila Lopez, Jessica
Guilloy, Noé
Castonguay, Anne
Husain, Mohammed Amir
Germain, Joannie St.
Boisvert, François-Michel
Plourde, Mélanie
Roucou, Xavier
Laurent, Benoit
author_facet Khatir, Inès
Brunet, Marie A.
Meller, Anna
Amiot, Florent
Patel, Tushar
Lapointe, Xavier
Avila Lopez, Jessica
Guilloy, Noé
Castonguay, Anne
Husain, Mohammed Amir
Germain, Joannie St.
Boisvert, François-Michel
Plourde, Mélanie
Roucou, Xavier
Laurent, Benoit
author_sort Khatir, Inès
collection PubMed
description During aging, changes in gene expression are associated with a decline in physical and cognitive abilities. Here, we investigate the connection between changes in mRNA and protein expression in the brain by comparing the transcriptome and proteome of the mouse cortex during aging. Our transcriptomic analysis revealed that aging mainly triggers gene activation in the cortex. We showed that an increase in mRNA expression correlates with protein expression, specifically in the anterior cingulate cortex, where we also observed an increase in cortical thickness during aging. Genes exhibiting an aging-dependent increase of mRNA and protein levels are involved in sensory perception and immune functions. Our proteomic analysis also identified changes in protein abundance in the aging cortex and highlighted a subset of proteins that were differentially enriched but exhibited stable mRNA levels during aging, implying the contribution of aging-related post- transcriptional and post-translational mechanisms. These specific genes were associated with general biological processes such as translation, ribosome assembly and protein degradation, and also important brain functions related to neuroplasticity. By decoupling mRNA and protein expression, we have thus characterized distinct subsets of genes that differentially adjust to cellular aging in the cerebral cortex.
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spelling pubmed-99540252023-02-25 Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets Khatir, Inès Brunet, Marie A. Meller, Anna Amiot, Florent Patel, Tushar Lapointe, Xavier Avila Lopez, Jessica Guilloy, Noé Castonguay, Anne Husain, Mohammed Amir Germain, Joannie St. Boisvert, François-Michel Plourde, Mélanie Roucou, Xavier Laurent, Benoit Cells Article During aging, changes in gene expression are associated with a decline in physical and cognitive abilities. Here, we investigate the connection between changes in mRNA and protein expression in the brain by comparing the transcriptome and proteome of the mouse cortex during aging. Our transcriptomic analysis revealed that aging mainly triggers gene activation in the cortex. We showed that an increase in mRNA expression correlates with protein expression, specifically in the anterior cingulate cortex, where we also observed an increase in cortical thickness during aging. Genes exhibiting an aging-dependent increase of mRNA and protein levels are involved in sensory perception and immune functions. Our proteomic analysis also identified changes in protein abundance in the aging cortex and highlighted a subset of proteins that were differentially enriched but exhibited stable mRNA levels during aging, implying the contribution of aging-related post- transcriptional and post-translational mechanisms. These specific genes were associated with general biological processes such as translation, ribosome assembly and protein degradation, and also important brain functions related to neuroplasticity. By decoupling mRNA and protein expression, we have thus characterized distinct subsets of genes that differentially adjust to cellular aging in the cerebral cortex. MDPI 2023-02-14 /pmc/articles/PMC9954025/ /pubmed/36831282 http://dx.doi.org/10.3390/cells12040615 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khatir, Inès
Brunet, Marie A.
Meller, Anna
Amiot, Florent
Patel, Tushar
Lapointe, Xavier
Avila Lopez, Jessica
Guilloy, Noé
Castonguay, Anne
Husain, Mohammed Amir
Germain, Joannie St.
Boisvert, François-Michel
Plourde, Mélanie
Roucou, Xavier
Laurent, Benoit
Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets
title Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets
title_full Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets
title_fullStr Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets
title_full_unstemmed Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets
title_short Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets
title_sort decoupling of mrna and protein expression in aging brains reveals the age-dependent adaptation of specific gene subsets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954025/
https://www.ncbi.nlm.nih.gov/pubmed/36831282
http://dx.doi.org/10.3390/cells12040615
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