Cargando…

Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia

Transcutaneous auricular vagus nerve stimulation was recently reported to have a therapeutic potential for functional dyspepsia (FD). This study aimed to explore the integrative effects and mechanisms of auricular vagus nerve stimulation (aVNS) in a rodent model of FD. Methods: We evaluated the effe...

Descripción completa

Detalles Bibliográficos
Autores principales: Hou, Liwei, Rong, Peijing, Yang, Yang, Fang, Jiliang, Wang, Junying, Wang, Yu, Zhang, Jinling, Zhang, Shuai, Zhang, Zixuan, Chen, Jiande D. Z., Wei, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954117/
https://www.ncbi.nlm.nih.gov/pubmed/36831796
http://dx.doi.org/10.3390/brainsci13020253
_version_ 1784894047528157184
author Hou, Liwei
Rong, Peijing
Yang, Yang
Fang, Jiliang
Wang, Junying
Wang, Yu
Zhang, Jinling
Zhang, Shuai
Zhang, Zixuan
Chen, Jiande D. Z.
Wei, Wei
author_facet Hou, Liwei
Rong, Peijing
Yang, Yang
Fang, Jiliang
Wang, Junying
Wang, Yu
Zhang, Jinling
Zhang, Shuai
Zhang, Zixuan
Chen, Jiande D. Z.
Wei, Wei
author_sort Hou, Liwei
collection PubMed
description Transcutaneous auricular vagus nerve stimulation was recently reported to have a therapeutic potential for functional dyspepsia (FD). This study aimed to explore the integrative effects and mechanisms of auricular vagus nerve stimulation (aVNS) in a rodent model of FD. Methods: We evaluated the effects of aVNS on visceral hypersensitivity, gastric motility and open field test (OFT) activity in iodoacetamide (IA)-treated rats. The autonomic function was assessed; blood samples and tissues were collected and analyzed by an enzyme-linked immunosorbent assay and western blot. Vagotomy was performed to investigate the role of vagal efferent nerve. Results: aVNS reduced the electromyography response to gastric distension, improved gastric emptying and increased the horizontal and vertical motion scores of the OFT in IA-treated rats. The sympathovagal ratio was increased in IA-treated rats but normalized with aVNS. The serum cytokines TNF-α, IL-6, IL-1β and NF-κBp65 were increased in IA-treated rats and decreased with aVNS. The hypothalamus–pituitary–adrenal axis was hyperactive in IA-treated rats but inhibited by aVNS. The expression of duodenal desmoglein 2 and occludin were all decreased in IA-treated rats and increased with aVNS but not sham-aVNS. Vagotomy abolished the ameliorating effects of aVNS on gastric emptying, horizontal motions, serum TNF-α and duodenal NF-κBp65. Conclusion: aVNS improves gastric motility and gastric hypersensitivity probably by anti-inflammatory mechanisms via the vago-vagal pathways. A better understanding on the mechanisms of action involved with aVNS would lead to the optimization of the taVNS methodology and promote taVNS as a non-pharmacological alternative therapy for FD.
format Online
Article
Text
id pubmed-9954117
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99541172023-02-25 Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia Hou, Liwei Rong, Peijing Yang, Yang Fang, Jiliang Wang, Junying Wang, Yu Zhang, Jinling Zhang, Shuai Zhang, Zixuan Chen, Jiande D. Z. Wei, Wei Brain Sci Article Transcutaneous auricular vagus nerve stimulation was recently reported to have a therapeutic potential for functional dyspepsia (FD). This study aimed to explore the integrative effects and mechanisms of auricular vagus nerve stimulation (aVNS) in a rodent model of FD. Methods: We evaluated the effects of aVNS on visceral hypersensitivity, gastric motility and open field test (OFT) activity in iodoacetamide (IA)-treated rats. The autonomic function was assessed; blood samples and tissues were collected and analyzed by an enzyme-linked immunosorbent assay and western blot. Vagotomy was performed to investigate the role of vagal efferent nerve. Results: aVNS reduced the electromyography response to gastric distension, improved gastric emptying and increased the horizontal and vertical motion scores of the OFT in IA-treated rats. The sympathovagal ratio was increased in IA-treated rats but normalized with aVNS. The serum cytokines TNF-α, IL-6, IL-1β and NF-κBp65 were increased in IA-treated rats and decreased with aVNS. The hypothalamus–pituitary–adrenal axis was hyperactive in IA-treated rats but inhibited by aVNS. The expression of duodenal desmoglein 2 and occludin were all decreased in IA-treated rats and increased with aVNS but not sham-aVNS. Vagotomy abolished the ameliorating effects of aVNS on gastric emptying, horizontal motions, serum TNF-α and duodenal NF-κBp65. Conclusion: aVNS improves gastric motility and gastric hypersensitivity probably by anti-inflammatory mechanisms via the vago-vagal pathways. A better understanding on the mechanisms of action involved with aVNS would lead to the optimization of the taVNS methodology and promote taVNS as a non-pharmacological alternative therapy for FD. MDPI 2023-02-01 /pmc/articles/PMC9954117/ /pubmed/36831796 http://dx.doi.org/10.3390/brainsci13020253 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hou, Liwei
Rong, Peijing
Yang, Yang
Fang, Jiliang
Wang, Junying
Wang, Yu
Zhang, Jinling
Zhang, Shuai
Zhang, Zixuan
Chen, Jiande D. Z.
Wei, Wei
Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia
title Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia
title_full Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia
title_fullStr Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia
title_full_unstemmed Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia
title_short Auricular Vagus Nerve Stimulation Improves Visceral Hypersensitivity and Gastric Motility and Depression-like Behaviors via Vago-Vagal Pathway in a Rat Model of Functional Dyspepsia
title_sort auricular vagus nerve stimulation improves visceral hypersensitivity and gastric motility and depression-like behaviors via vago-vagal pathway in a rat model of functional dyspepsia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954117/
https://www.ncbi.nlm.nih.gov/pubmed/36831796
http://dx.doi.org/10.3390/brainsci13020253
work_keys_str_mv AT houliwei auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT rongpeijing auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT yangyang auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT fangjiliang auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT wangjunying auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT wangyu auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT zhangjinling auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT zhangshuai auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT zhangzixuan auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT chenjiandedz auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia
AT weiwei auricularvagusnervestimulationimprovesvisceralhypersensitivityandgastricmotilityanddepressionlikebehaviorsviavagovagalpathwayinaratmodeloffunctionaldyspepsia