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A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging

We reported on an efficient RNA imaging strategy based on a CRISPR-Cas and Tat peptide with a fluorescent RNA aptamer (TRAP-tag). Using modified CRISPR-Cas RNA hairpin binding proteins fused with a Tat peptide array that recruits modified RNA aptamers, this simple and sensitive strategy is capable o...

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Detalles Bibliográficos
Autores principales: Tang, Heng, Peng, Junran, Jiang, Xin, Peng, Shuang, Wang, Fang, Weng, Xiaocheng, Zhou, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954185/
https://www.ncbi.nlm.nih.gov/pubmed/36832059
http://dx.doi.org/10.3390/bios13020293
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author Tang, Heng
Peng, Junran
Jiang, Xin
Peng, Shuang
Wang, Fang
Weng, Xiaocheng
Zhou, Xiang
author_facet Tang, Heng
Peng, Junran
Jiang, Xin
Peng, Shuang
Wang, Fang
Weng, Xiaocheng
Zhou, Xiang
author_sort Tang, Heng
collection PubMed
description We reported on an efficient RNA imaging strategy based on a CRISPR-Cas and Tat peptide with a fluorescent RNA aptamer (TRAP-tag). Using modified CRISPR-Cas RNA hairpin binding proteins fused with a Tat peptide array that recruits modified RNA aptamers, this simple and sensitive strategy is capable of visualizing endogenous RNA in cells with high precision and efficiency. In addition, the modular design of the CRISPR-TRAP-tag facilitates the substitution of sgRNAs, RNA hairpin binding proteins, and aptamers in order to optimize imaging quality and live cell affinity. With CRISPR-TRAP-tag, exogenous GCN4, endogenous mRNA MUC4, and lncRNA SatIII were distinctly visualized in single live cells.
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spelling pubmed-99541852023-02-25 A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging Tang, Heng Peng, Junran Jiang, Xin Peng, Shuang Wang, Fang Weng, Xiaocheng Zhou, Xiang Biosensors (Basel) Article We reported on an efficient RNA imaging strategy based on a CRISPR-Cas and Tat peptide with a fluorescent RNA aptamer (TRAP-tag). Using modified CRISPR-Cas RNA hairpin binding proteins fused with a Tat peptide array that recruits modified RNA aptamers, this simple and sensitive strategy is capable of visualizing endogenous RNA in cells with high precision and efficiency. In addition, the modular design of the CRISPR-TRAP-tag facilitates the substitution of sgRNAs, RNA hairpin binding proteins, and aptamers in order to optimize imaging quality and live cell affinity. With CRISPR-TRAP-tag, exogenous GCN4, endogenous mRNA MUC4, and lncRNA SatIII were distinctly visualized in single live cells. MDPI 2023-02-18 /pmc/articles/PMC9954185/ /pubmed/36832059 http://dx.doi.org/10.3390/bios13020293 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Heng
Peng, Junran
Jiang, Xin
Peng, Shuang
Wang, Fang
Weng, Xiaocheng
Zhou, Xiang
A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
title A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
title_full A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
title_fullStr A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
title_full_unstemmed A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
title_short A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
title_sort crispr-cas and tat peptide with fluorescent rna aptamer system for signal amplification in rna imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954185/
https://www.ncbi.nlm.nih.gov/pubmed/36832059
http://dx.doi.org/10.3390/bios13020293
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