Cargando…

Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection

This study reports the synthesis of a nanocomposite consisting of spongin and its applicability in the development of an aptasensing platform with high performance. The spongin was carefully extracted from a marine sponge and decorated with copper tungsten oxide hydroxide. The resulting spongin-copp...

Descripción completa

Detalles Bibliográficos
Autores principales: Shahdost-Fard, Faezeh, Faridfar, Shahin, Keihan, Amir Homayoun, Aghaei, Mohammad, Petrenko, Iaroslav, Ahmadi, Farhad, Ehrlich, Hermann, Rahimi-Nasrabadi, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954421/
https://www.ncbi.nlm.nih.gov/pubmed/36832038
http://dx.doi.org/10.3390/bios13020271
_version_ 1784894113270726656
author Shahdost-Fard, Faezeh
Faridfar, Shahin
Keihan, Amir Homayoun
Aghaei, Mohammad
Petrenko, Iaroslav
Ahmadi, Farhad
Ehrlich, Hermann
Rahimi-Nasrabadi, Mehdi
author_facet Shahdost-Fard, Faezeh
Faridfar, Shahin
Keihan, Amir Homayoun
Aghaei, Mohammad
Petrenko, Iaroslav
Ahmadi, Farhad
Ehrlich, Hermann
Rahimi-Nasrabadi, Mehdi
author_sort Shahdost-Fard, Faezeh
collection PubMed
description This study reports the synthesis of a nanocomposite consisting of spongin and its applicability in the development of an aptasensing platform with high performance. The spongin was carefully extracted from a marine sponge and decorated with copper tungsten oxide hydroxide. The resulting spongin-copper tungsten oxide hydroxide was functionalized by silver nanoparticles and utilized in electrochemical aptasensor fabrication. The nanocomposite covered on a glassy carbon electrode surface amplified the electron transfer and increased active electrochemical sites. The aptasensor was fabricated by loading of thiolated aptamer on the embedded surface via thiol-AgNPs linkage. The applicability of the aptasensor was tested in detecting the Staphylococcus aureus bacterium as one of the five most common causes of nosocomial infectious diseases. The aptasensor measured S. aureus under a linear concentration range of 10–10(8) colony-forming units per milliliter and a limit of quantification and detection of 12 and 1 colony-forming unit per milliliter, respectively. The highly selective diagnosis of S. aureus in the presence of some common bacterial strains was satisfactorily evaluated. The acceptable results of the human serum analysis as the real sample may be promising in the bacteria tracking in clinical samples underlying the green chemistry principle.
format Online
Article
Text
id pubmed-9954421
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-99544212023-02-25 Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection Shahdost-Fard, Faezeh Faridfar, Shahin Keihan, Amir Homayoun Aghaei, Mohammad Petrenko, Iaroslav Ahmadi, Farhad Ehrlich, Hermann Rahimi-Nasrabadi, Mehdi Biosensors (Basel) Article This study reports the synthesis of a nanocomposite consisting of spongin and its applicability in the development of an aptasensing platform with high performance. The spongin was carefully extracted from a marine sponge and decorated with copper tungsten oxide hydroxide. The resulting spongin-copper tungsten oxide hydroxide was functionalized by silver nanoparticles and utilized in electrochemical aptasensor fabrication. The nanocomposite covered on a glassy carbon electrode surface amplified the electron transfer and increased active electrochemical sites. The aptasensor was fabricated by loading of thiolated aptamer on the embedded surface via thiol-AgNPs linkage. The applicability of the aptasensor was tested in detecting the Staphylococcus aureus bacterium as one of the five most common causes of nosocomial infectious diseases. The aptasensor measured S. aureus under a linear concentration range of 10–10(8) colony-forming units per milliliter and a limit of quantification and detection of 12 and 1 colony-forming unit per milliliter, respectively. The highly selective diagnosis of S. aureus in the presence of some common bacterial strains was satisfactorily evaluated. The acceptable results of the human serum analysis as the real sample may be promising in the bacteria tracking in clinical samples underlying the green chemistry principle. MDPI 2023-02-14 /pmc/articles/PMC9954421/ /pubmed/36832038 http://dx.doi.org/10.3390/bios13020271 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shahdost-Fard, Faezeh
Faridfar, Shahin
Keihan, Amir Homayoun
Aghaei, Mohammad
Petrenko, Iaroslav
Ahmadi, Farhad
Ehrlich, Hermann
Rahimi-Nasrabadi, Mehdi
Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection
title Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection
title_full Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection
title_fullStr Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection
title_full_unstemmed Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection
title_short Applicability of a Green Nanocomposite Consisted of Spongin Decorated Cu(2)WO(4)(OH)(2) and AgNPs as a High-Performance Aptasensing Platform in Staphylococcus aureus Detection
title_sort applicability of a green nanocomposite consisted of spongin decorated cu(2)wo(4)(oh)(2) and agnps as a high-performance aptasensing platform in staphylococcus aureus detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9954421/
https://www.ncbi.nlm.nih.gov/pubmed/36832038
http://dx.doi.org/10.3390/bios13020271
work_keys_str_mv AT shahdostfardfaezeh applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection
AT faridfarshahin applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection
AT keihanamirhomayoun applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection
AT aghaeimohammad applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection
AT petrenkoiaroslav applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection
AT ahmadifarhad applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection
AT ehrlichhermann applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection
AT rahiminasrabadimehdi applicabilityofagreennanocompositeconsistedofspongindecoratedcu2wo4oh2andagnpsasahighperformanceaptasensingplatforminstaphylococcusaureusdetection